Calmodulin‐binding transcription activator (CAMTA) 3 mediates biotic defense responses in <i>Arabidopsis</i>

Galon, Yael; Nave, Roy; Boyce, Joy M.; Nachmias, Dikla; Knight, Marc R.; Fromm, Hillel

doi:10.1016/j.febslet.2008.02.037

Cited by 191 publications

(204 citation statements)

References 20 publications

(25 reference statements)

Supporting

Mentioning

194

Contrasting

Order By: Relevance

“…Thus, CAMTA proteins may be involved in the expression of many abiotic and biotic stress genes. Consistent with this idea is the recent finding of Galon et al (2008) indicating that CAMTA3 is a suppressor of defense responses in Arabidopsis.…”

Section: Camta Proteins As Early Sensors Of Stresssupporting

confidence: 76%

Roles forArabidopsisCAMTA Transcription Factors in Cold-Regulated Gene Expression and Freezing Tolerance

et al. 2009

View full text Add to dashboard Cite

The Arabidopsis thaliana CBF cold response pathway plays a central role in cold acclimation. It is characterized by rapid cold induction of genes encoding the CBF1-3 transcription factors, followed by expression of the CBF gene regulon, which imparts freezing tolerance. Our goal was to further the understanding of the cis-acting elements and trans-acting factors involved in expression of CBF2. We identified seven conserved DNA motifs (CM), CM1 to 7, that are present in the promoters of CBF2 and another rapidly cold-induced gene encoding a transcription factor, ZAT12. The results presented indicate that in the CBF2 promoter, CM4 and CM6 have negative regulatory activity and that CM2 has both negative and positive activity. A Myc binding site in the CBF2 promoter was also found to have positive regulatory effects. Moreover, our results indicate that members of the calmodulin binding transcription activator (CAMTA) family of transcription factors bind to the CM2 motif, that CAMTA3 is a positive regulator of CBF2 expression, and that double camta1 camta3 mutant plants are impaired in freezing tolerance. These results establish a role for CAMTA proteins in cold acclimation and provide a possible point of integrating low-temperature calcium and calmodulin signaling with cold-regulated gene expression.

show abstract

Section: Camta Proteins As Early Sensors Of Stresssupporting

confidence: 76%

Roles forArabidopsisCAMTA Transcription Factors in Cold-Regulated Gene Expression and Freezing Tolerance

et al. 2009

View full text Add to dashboard Cite

show abstract

“…It is not clear what other signals induce the CAM box, although a role for the CAMTA transcription factors that bind this motif has also been shown in response to pathogens and auxin (Galon et al, 2008;Du et al, 2009;Galon et al, 2010b), which also provoke elevations in [Ca 2+ ] c (Felle, 1988;Knight et al, 1996;Grant et al, 2000). The CRT element is also induced in response to drought (Liu et al, 1998), and the ABRE is induced in response to ABA and drought (Hobo et al, 1999), which both induce [Ca 2+ ] c (McAinsh et al, 1990;Knight et al, 1996;Knight et al, 1997).…”

Section: Identification Of Four Promoter Motifs That Are Overrepresenmentioning

confidence: 98%

“…The expression of specific stress-responsive genes has been demonstrated to be calciumregulated, for example, in response to cold (Knight et al, 1996;Knight et al, 1997;Tä htiharju et al, 1997;Galon et al, 2010a). The protein intermediates brokering calcium signals to regulate gene expression have also, in some cases, been identified, for example calmodulins (CaMs) (Takahashi et al, 2011), CaM-like proteins (Chiasson et al, 2005;Magnan et al, 2008;Xu et al, 2011), calcium-dependent protein kinases (CPKs) (Boudsocq et al, 2010;Coca and Segundo, 2010), CIPK/CBLs (Albrecht et al, 2003;Weinl and Kudla, 2009), and even transcription factors, such as CaM-binding transcription activators (CAMTAs) (Galon et al, 2008;Doherty et al, 2009;Du et al, 2009;Galon et al, 2010b).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis Reveals Calcium Regulation of Specific Promoter Motifs inArabidopsis

et al. 2011

Self Cite

View full text Add to dashboard Cite

Increases in intracellular calcium concentration ([Ca 2+ ] c ) mediate plant responses to stress by regulating the expression of genes encoding proteins that confer tolerance. Several plant stress genes have previously been shown to be calciumregulated, and in one case, a specific promoter motif Abscisic Acid Responsive-Element (ABRE) has been found to be regulated by calcium. A comprehensive survey of the Arabidopsis thaliana transcriptome for calcium-regulated promoter motifs was performed by measuring the expression of genes in Arabidopsis seedlings responding to three calcium elevations of different characteristics, using full genome microarray analysis. This work revealed a total of 269 genes upregulated by [Ca 2+ ] c in Arabidopsis. Bioinformatic analysis strongly indicated that at least four promoter motifs were [Ca 2+ ] c -regulated in planta. We confirmed this finding by expressing in plants chimeric gene constructs controlled exclusively by these cis-elements and by testing the necessity and sufficiency of calcium for their expression. Our data reveal that the C-Repeat/Drought-Responsive Element, Site II, and CAM box (along with the previously identified ABRE) promoter motifs are calcium-regulated. The identification of these promoter elements targeted by the second messenger intracellular calcium has implications for plant signaling in response to a variety of stimuli, including cold, drought, and biotic stress.

show abstract

“…Primers for PR1 (At2g14610; F, 5#-TCGTCTTTGTAGCTCTTGTAGGTG-3#, and R, 5#-TTCATTAGTATGGCT-TCTCGTTCA-3#) and WRKY70 (At3g56400; F, 5#-TAATGGATACTAATAAA-GCAAAAAAGC-3#, and R, 5#-CAGATAGATTCGAACATGAACTGAAG-3#) were described by Ü lker et al (2007). Primers for RLK5 (At4g23140; F, 5#-TAAAGCAAGTAACATTCTCCTAGATGC-3#, and R, 5#-AGCATGAAGT-TTAAGGTCATATTTAGC-3#) were described by Galon et al (2008). Primers for gene At5g10760 were designed as follows: F, 5#-CTTGTTGTGAGAGTAA-GAGGGAGGT-3#, and R, 5#-CCGCAACCAAAGTAAACATCCTCCA-3#.…”

Section: Lipid Peroxidationmentioning

confidence: 99%

Leaf Senescence Signaling: The Ca2+-Conducting Arabidopsis Cyclic Nucleotide Gated Channel2 Acts through Nitric Oxide to Repress Senescence Programming

Smigel

Walker

et al. 2010

Plant Physiology

View full text Add to dashboard Cite

Ca 2+ and nitric oxide (NO) are essential components involved in plant senescence signaling cascades. In other signaling pathways, NO generation can be dependent on cytosolic Ca 2+ . The Arabidopsis (Arabidopsis thaliana) mutant dnd1 lacks a plasma membrane-localized cation channel (CNGC2). We recently demonstrated that this channel affects plant response to pathogens through a signaling cascade involving Ca 2+ modulation of NO generation; the pathogen response phenotype of dnd1 can be complemented by application of a NO donor. At present, the interrelationship between Ca 2+ and NO generation in plant cells during leaf senescence remains unclear. Here, we use dnd1 plants to present genetic evidence consistent with the hypothesis that Ca 2+ uptake and NO production play pivotal roles in plant leaf senescence. Leaf Ca 2+ accumulation is reduced in dnd1 leaves compared to the wild type. Early senescence-associated phenotypes (such as loss of chlorophyll, expression level of senescence-associated genes, H 2 O 2 generation, lipid peroxidation, tissue necrosis, and increased salicylic acid levels) were more prominent in dnd1 leaves compared to the wild type. Application of a Ca 2+ channel blocker hastened senescence of detached wild-type leaves maintained in the dark, increasing the rate of chlorophyll loss, expression of a senescence-associated gene, and lipid peroxidation. Pharmacological manipulation of Ca 2+ signaling provides evidence consistent with genetic studies of the relationship between Ca 2+ signaling and senescence with the dnd1 mutant. Basal levels of NO in dnd1 leaf tissue were lower than that in leaves of wild-type plants. Application of a NO donor effectively rescues many dnd1 senescence-related phenotypes. Our work demonstrates that the CNGC2 channel is involved in Ca 2+ uptake during plant development beyond its role in pathogen defense response signaling. Work presented here suggests that this function of CNGC2 may impact downstream basal NO production in addition to its role (also linked to NO signaling) in pathogen defense responses and that this NO generation acts as a negative regulator during plant leaf senescence signaling.

show abstract

Calmodulin‐binding transcription activator (CAMTA) 3 mediates biotic defense responses in Arabidopsis

Cited by 191 publications

References 20 publications

Roles forArabidopsisCAMTA Transcription Factors in Cold-Regulated Gene Expression and Freezing Tolerance

Roles forArabidopsisCAMTA Transcription Factors in Cold-Regulated Gene Expression and Freezing Tolerance

Transcriptomic Analysis Reveals Calcium Regulation of Specific Promoter Motifs inArabidopsis

Leaf Senescence Signaling: The Ca2+-Conducting Arabidopsis Cyclic Nucleotide Gated Channel2 Acts through Nitric Oxide to Repress Senescence Programming

Contact Info

Product

Resources

About