Roles for<i>Arabidopsis</i>CAMTA Transcription Factors in Cold-Regulated Gene Expression and Freezing Tolerance

Doherty, Colleen J.; Buskirk, Heather A. Van; Myers, Susan J.; Thomashow, Michael F.

doi:10.1105/tpc.108.063958

Cited by 572 publications

(568 citation statements)

References 41 publications

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“…The expression of specific stress-responsive genes has been demonstrated to be calciumregulated, for example, in response to cold (Knight et al, 1996;Knight et al, 1997;Tä htiharju et al, 1997;Galon et al, 2010a). The protein intermediates brokering calcium signals to regulate gene expression have also, in some cases, been identified, for example calmodulins (CaMs) (Takahashi et al, 2011), CaM-like proteins (Chiasson et al, 2005;Magnan et al, 2008;Xu et al, 2011), calcium-dependent protein kinases (CPKs) (Boudsocq et al, 2010;Coca and Segundo, 2010), CIPK/CBLs (Albrecht et al, 2003;Weinl and Kudla, 2009), and even transcription factors, such as CaM-binding transcription activators (CAMTAs) (Galon et al, 2008;Doherty et al, 2009;Du et al, 2009;Galon et al, 2010b).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis Reveals Calcium Regulation of Specific Promoter Motifs inArabidopsis

et al. 2011

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Increases in intracellular calcium concentration ([Ca 2+ ] c ) mediate plant responses to stress by regulating the expression of genes encoding proteins that confer tolerance. Several plant stress genes have previously been shown to be calciumregulated, and in one case, a specific promoter motif Abscisic Acid Responsive-Element (ABRE) has been found to be regulated by calcium. A comprehensive survey of the Arabidopsis thaliana transcriptome for calcium-regulated promoter motifs was performed by measuring the expression of genes in Arabidopsis seedlings responding to three calcium elevations of different characteristics, using full genome microarray analysis. This work revealed a total of 269 genes upregulated by [Ca 2+ ] c in Arabidopsis. Bioinformatic analysis strongly indicated that at least four promoter motifs were [Ca 2+ ] c -regulated in planta. We confirmed this finding by expressing in plants chimeric gene constructs controlled exclusively by these cis-elements and by testing the necessity and sufficiency of calcium for their expression. Our data reveal that the C-Repeat/Drought-Responsive Element, Site II, and CAM box (along with the previously identified ABRE) promoter motifs are calcium-regulated. The identification of these promoter elements targeted by the second messenger intracellular calcium has implications for plant signaling in response to a variety of stimuli, including cold, drought, and biotic stress.

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Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis Reveals Calcium Regulation of Specific Promoter Motifs inArabidopsis

et al. 2011

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“…In Arabidopsis, CBF1-3 are the primary cold inducible transcription factors [12,13], with AtCBF3 and AtCBF1-2 being first transcriptionally activated by ICE-1 and CAMTA, respectively [44][45][46]. There are five potential elements (myc-recognition sites, CANNTG) present in the promoter (~1kb from the ATG) of AtCBF3 [44,47] to which ICE1 could bind.…”

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confidence: 99%

Functionality of soybean CBF/DREB1 transcription factors

Yamasaki

Randall

2016

Plant Science

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Soybean (Glycine max) is considered to be cold intolerant and is not able to significantly acclimate to cold/freezing stress. In most cold tolerant plants, the Crepeat/DRE Binding Factors (CBF/DREBs) are critical contributors to successful cold-responses; rapidly increasing following cold treatment and regulating the induction of many cold responsive genes. In soybean vegetative tissue, we found strong, transient accumulation of CBF transcripts in response to cold stress; however, the soybean transcripts of typical cold responsive genes (homologues to Arabidopsis genes such as dehydrins, ADH1, RAP2.1, and LEA14) were not significantly altered.Soybean CBFs were found to be functional, as when expressed constitutively in Arabidopsis they increased the levels of AtCOR47 and AtRD29a transcripts and increased freezing tolerance as measured by a decrease in leaf freezing damage and ion leakage. Furthermore the constitutive expression of GmDREB1A;2 and GmDREB1B;1 in Arabidopsis led to stronger up-regulation of downstream genes and more freezing tolerance than GmDREB1A;1, the gene whose transcript is the major contributor to total CBF/DREB1 transcripts in soybean. The inability for the soybean CBFs to significantly up regulate the soybean genes that contribute to cold tolerance is consistent with poor acclimation capability and the cold intolerance of soybean.

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“…Accordingly, mutation of the Ca 2+ /H + antiporter CALCIUM EXCHANGER1 enhances freezing tolerance following cold acclimation and increases CBF gene expression (Catalá et al, 2003). The calmodulin-binding transcriptional activator TF family also is involved in the regulation of CBF genes by binding to the CM2 motif in the AtCBF2 promoter and increasing its expression (Doherty et al, 2009). AtCBF3 expression is induced by a basic helix-loop-helix TF, INDUCER OF CBF EXPRESSION1 (ICE1), and is negatively regulated by MYB15 (Chinnusamy et al, 2003;Agarwal et al, 2006).…”

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confidence: 99%

Opposing control by transcription factors MYB61 and MYB3 Increases Freezing Tolerance by relieving C-repeat Binding Factor suppression

Zhang

et al. 2016

Plant Physiol.

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ORCID IDs: 0000-0003-0210-9408 (Z.Z.); 0000-0002-6839-3272 (X.H.); 0000-0001-5113-7750 (J.W.); 0000-0002-5098-8681 (J.D.).Cold acclimation is an important process by which plants respond to low temperature and enhance their winter hardiness. C-REPEAT BINDING FACTOR1 (CBF1), CBF2, and CBF3 genes were shown previously to participate in cold acclimation in Medicago truncatula. In addition, MtCBF4 is transcriptionally induced by salt, drought, and cold stresses. We show here that MtCBF4, shown previously to enhance drought and salt tolerance, also positively regulates cold acclimation and freezing tolerance. To identify molecular factors acting upstream and downstream of the MtCBF4 transcription factor (TF) in cold responses, we first identified genes that are differentially regulated upon MtCBF4 overexpression using RNAseq Digital Gene Expression Profiling. Among these, we showed that MtCBF4 directly activates the transcription of the COLD ACCLIMATION SPECIFIC15 (MtCAS15) gene. To gain insights into how MtCBF4 is transcriptionally regulated in response to cold, an R2R3-MYB TF, MtMYB3, was identified based on a yeast one-hybrid screen as binding directly to MYB cis-elements in the MtCBF4 promoter, leading to the inhibition of MtCBF4 expression. In addition, another MYB TF, MtMYB61, identified as an interactor of MtMYB3, can relieve the inhibitory effect of MtMYB3 on MtCBF4 transcription. This study, therefore, supports a model describing how MtCBF4 is regulated by antagonistic MtMYB3/MtMYB61 TFs, leading to the up-regulation of downstream targets such as MtCAS15 acting in cold acclimation in M. truncatula.

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Roles forArabidopsisCAMTA Transcription Factors in Cold-Regulated Gene Expression and Freezing Tolerance

Cited by 572 publications

References 41 publications

Transcriptomic Analysis Reveals Calcium Regulation of Specific Promoter Motifs inArabidopsis

Transcriptomic Analysis Reveals Calcium Regulation of Specific Promoter Motifs inArabidopsis

Functionality of soybean CBF/DREB1 transcription factors

Opposing control by transcription factors MYB61 and MYB3 Increases Freezing Tolerance by relieving C-repeat Binding Factor suppression

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