Callus Maintenance and Cell Line Selection of <i>Grewia Tenax</i>

Daffalla, Hussien M.; Elsheikh, Azza M.; Ali, Hiba A.; Khalafalla, Mutasim M.

doi:10.1080/10496475.2019.1595256

Cited by 5 publications

(5 citation statements)

References 14 publications

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“…In addition, high NAA concentrations were used successfully in promoting callus growth in Piper longum [29]. In another study, Daffalla et al [30] found that a combination of 10.74 µM NAA with 6.66 µM BAP increased the callus growth of Grewia tenax, and this is in general agreement with the results of this study.…”

Section: Gileadensis Callus Culturesupporting

confidence: 91%

Secondary Metabolites Profiling, Antimicrobial and Cytotoxic Properties of Commiphora gileadensis L. Leaves, Seeds, Callus, and Cell Suspension Extracts

et al. 2023

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Commiphora gileadensis L. is an important endangered medicinal plant that belongs to the family Burseraceae. In this study, C. gileadensis callus culture was established successfully using mature leaves as explants cultured on Murashige and Skoog (MS) media supplemented with 24.50 μM of indole butyric acid (IBA) and 2.22 μM 6-Benzylaminopurine (BAP) (callus induction media). The obtained callus was maintained on MS medium supplemented with 16.11 μM naphthalene acetic acid (NAA) in combination with 6.66 μM BAP, which resulted in a substantial increase in callus fresh and dry weights. The cell suspension culture was established successfully using liquid callus induction media supplemented with 3.0 mg·L−1 proline. Thereafter, the chemical constituents of different C. gileadensis methanolic extracts (callus, cell suspension, leaves, and seeds) were profiled, and their cytotoxic and antimicrobial properties were investigated. The LC-MS GNPS analyses were applied for chemical profiling of the methanolic plant extracts, and several natural products were identified, including flavonols, flavanones, and flavonoids glycosides, with two unusual families that included puromycin, 10-hydroxycamptothecin, and justicidin B. The methanolic extracts have shown selective antimicrobial and cytotoxic properties against different microbes and cancer cell lines. For instance, leaf extract showed the highest zone of inhibition for Staphylococcus aureus, while cell suspension culture was effective against Staphylococcus epidermidis and Staphylococcus aureus. All extracts showed selective activity against A549 cell lines for the cytotoxicity assay, while the leaf extract had a broad cytotoxic effect against all tested cell lines. This study revealed that C. gileadensis callus and cell suspension cultures can be employed to increase the in vitro formation of biologically active compounds that may have cytotoxicity and antibacterial action against different cancer cell lines and bacterial species. Further studies are required to isolate and identify such constituents that corroborate the observed activities.

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Section: Gileadensis Callus Culturesupporting

confidence: 91%

Secondary Metabolites Profiling, Antimicrobial and Cytotoxic Properties of Commiphora gileadensis L. Leaves, Seeds, Callus, and Cell Suspension Extracts

et al. 2023

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“…A growth curve encompasses phases that include lag phase, exponential phase, linear phase, and deceleration phase. The pattern of the growth curve is dependent on plant species (dos Santos and de Souza, 2016;Daffalla et al, 2019). The present study observed three growth stages; lag, exponential, and linear phase.…”

Section: Callus Growth Curvesupporting

confidence: 54%

“…ANOVA analysis indicated that 2,4-D (0.33 µM) recorded highest fresh weight (0.490 ±0.013 g FW) in Code 71 ( biomass. The prolonged linear phase suggests that a decrease in growth rate results from the scarcity of nutrients or drying of solidifying agent or accumulation of toxic substances (Daffalla et al, 2019). The results, therefore, suggest that transfer to new growth media is necessary to increase survival rates (cell viability) and sustain callus growth after the 42 nd day.…”

Section: Biomass Growth Measurementmentioning

confidence: 91%

“…The combination of 2,4-D + KIN (0.44 + 0.32 µM) yielded 0.304±0.012 g FW in Code 93 (Figure 2b).Callus growth curveThe callus growth curve is necessary to identify the suitable point of transfer in coffee tissue culture. The growth curve of calli indicated three distinct phases: lag phase, exponential phase, and linear phase (dos Santos and de Souza., 2016;Daffalla et al, 2019). Lag phase and exponential phase represent cell growth ideal for cell division and cell multiplication of callus.…”

mentioning

confidence: 99%

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Effects of genotype and plant growth regulators on callus induction in leaf cultures of Coffea arabica L. F1 hybrid

Mwaniki¹,

Lubabali²,

Asava³

et al. 2019

Afr. J. Biotechnol.

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Coffea arabica, F1 hybrid variety, Ruiru 11 is a highly sought after crop in Kenya due to its alleviated resistance to Coffee Berry Disease and Coffee Leaf Rust coupled with high yield capacity and good cup quality. Access to the variety's planting materials is limited due to challenges with difficulty in propagation using conventional methods of seed and vegetative propagation; and somatic embryogenesis is regarded as a suitable alternative propagation method. Therefore, the current study aimed to establish an induction protocol in F1 composite hybrid Ruiru 11. The current study investigated the effects of genotype and plant growth regulators, auxins and cytokinins, on induction of embryogenic callus in two composite genotypes of C. arabica L. F1 hybrid variety Ruiru 11. Leaf explants from the F1 hybrid were cultured on half-strength Murashige and Skoog (MS) media supplemented with varied concentrations and combinations of plant growth regulators. Callus formation was evaluated weekly until the 60 th day. Genotypic effects were assessed based on the difference on callus induction rates, biomass fresh weights and callus formation. The genotypes tested showed highest callus induction 88% (Code 71) and 100% (Code 93) with respect to the formation of embryogenic calli. Highest fresh weight was obtained at 0.973 ± 0.011 g in Code 71 and 0.649 ± 0.03 g in Code 93 in MS media supplemented with 2,4-D + BAP (0.53 + 0.11 µM). The observed results are useful in formulating the best growth regulator concentration suitable for mass in vitro propagation genotypes of Arabica coffee hybrid Ruiru 11 through callus induction in vitro of leaf explants.

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“…The callus growth curve aid in identifying the timeline for the highest callus biomass indicating subculturing period as the behaviour of callus cells differs at each phase of kinetics growth (Daffalla et al, 2019). Z.officinale var.…”

Section: Establishment Of the Callus Growth Curvementioning

confidence: 99%

Picloram enhanced the callus induction, growth kinetics, antioxidant potentials, and secondary metabolites production of Zingiber officinale var. rubrum callus cultures

Gnasekaran

Rahman

Chew

et al. 2023

Preprint

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Plant cells are driven by types and concentrations of plant growth regulators to produce callus mass containing bioactive compounds. This study aimed to induce callus and to observe the histological, phytochemicals, and antioxidant basis of the callus. An efficient callus induction protocol was developed using picloram for Malaysian red ginger, Zingiber officinale var. rubrum. The effect of auxinic picloram herbicide was studied using six different concentrations (0, 0.5, 1, 2, 4, and 8 mg/L) on various explants (leaf sheath, leaf, root) to optimise the callus induction. The induced callus was studied for growth kinetics, anatomical features, antioxidant capacity, and phytochemical content. The highest callogenesis frequency (93.75%) and biomass accumulation (3.68 g) were observed on leaf sheath explant cultured on ½ strength Murashige and Skoog (MS) medium supplemented with 8 mg/L which also requires earlier subculture duration (45 days post-inoculation) in comparison to lower concentrations. Morphological investigation through histological procedure demonstrated friable and non-embryogenic characteristics of the primary and subcultured callus. Cultivated leaf sheath (CLS) methanolic extract showed the highest total phenolic (191.26 mg GAE/g dry extract) and flavonoid (4.54 mg QE/g dry extract) contents contributing to antioxidant activity with an estimated EC50 of 0.208 mg/mL. Although comparatively lower than CLS extract, callus extracts showed higher antioxidant activity and significantly lower EC50 values than in vitro leaf sheath extract. 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, phenol, and phenolic glucoside were only present in callus cultures while methyl esters, fatty acids, and phytosterols could be obtained from leaf sheath and callus extracts. In conclusion, the callus culture of Z. officinale var. rubrum is a potential renewable source of bioactive phytochemical compounds and can be employed for biotechnological practices such as elicitor-induced accumulation of secondary metabolites and genetic modification.

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Callus Maintenance and Cell Line Selection of Grewia Tenax

Cited by 5 publications

References 14 publications

Secondary Metabolites Profiling, Antimicrobial and Cytotoxic Properties of Commiphora gileadensis L. Leaves, Seeds, Callus, and Cell Suspension Extracts

Secondary Metabolites Profiling, Antimicrobial and Cytotoxic Properties of Commiphora gileadensis L. Leaves, Seeds, Callus, and Cell Suspension Extracts

Effects of genotype and plant growth regulators on callus induction in leaf cultures of Coffea arabica L. F1 hybrid

Picloram enhanced the callus induction, growth kinetics, antioxidant potentials, and secondary metabolites production of Zingiber officinale var. rubrum callus cultures

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