Callus initiation and plant regeneration in ragi (Eleusine coracana Gaertn.)

Mohanty, B. D.; Gupta, S. Dutta; Ghosh, Parthadeb

doi:10.1007/bf00040311

Cited by 30 publications

(9 citation statements)

References 7 publications

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“…This is in conformity with earlier publications on kodo millet Rashid 2001, 2003). The induction of callus in cereals and millets is commonly achieved by 2,4-D (Mohanty et al 1985;Chandra and Kothari 1995;Kothari-Chajer et al 2008). However, in this study, addition of Kn or Zn to medium containing 2,4-D (9.0 lM) increased the percentage of EC induction from shoot apex explants with the optimal response achieved at 9.0 lM 2,4-D and 2.25 lM Kn.…”

Section: Discussionmentioning

confidence: 50%

Effects of cytokinins, carbohydrates and amino acids on induction and maturation of somatic embryos in kodo millet (Paspalum scorbiculatum Linn.)

Ceasar¹,

Ignacimuthu²

2010

Plant Cell Tiss Organ Cult

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The effects of cytokinins, carbohydrates and amino acids were studied on maturation and regeneration of embryogenic callus (EC) in kodo millet (Paspalum scorbiculatum Linn.) using a two-step culture procedure. The highest percentage (87.6%) of EC induction was obtained in the induction medium containing Murashige and Skoog (MS) basal salts supplemented with 9.0 lM 2,4-dichlorophenoxy acetic acid and 2.25 lM kinetin. This EC was subcultured in the maturation medium for somatic embryo (SE) maturation and plantlet formation. Addition of cytokinins, carbohydrates and amino acids in the maturation medium promoted the SE maturation and plantlet formation. The maturation medium containing MS basal salts amended with 4.50 lM thidiazuron, 120 mM maltose and 200 lM L-proline gave the maximum number of SEs (39.4) and plantlets (31.3). Plantlets were successfully grown to maturity after hardening in the soil.

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Section: Discussionmentioning

confidence: 50%

Effects of cytokinins, carbohydrates and amino acids on induction and maturation of somatic embryos in kodo millet (Paspalum scorbiculatum Linn.)

Ceasar¹,

Ignacimuthu²

2010

Plant Cell Tiss Organ Cult

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“…Since 1980, callus cultures capable of somatic embryogenesis have been reported in major cereals and millets such as Triticum aestivum, Zea mays, Secale cereale, Pennisetum americanum, Panicum miliaceum [20]. However, previous reports on regeneration in finger millet (Eleusine coracana GAERTN) has been through organogenesis [12,15] and multiple bud production [22]. Recently, we have published a preliminary report on somatic embryogenesis and plant regeneration from callus cultures derived from shoot apices of E. coracana [3].…”

Section: Introductionmentioning

confidence: 99%

Influence of phytohormones, carbohydrates, aminoacids, growth supplements and antibiotics on somatic embryogenesis and plant differentiation in finger millet

Eapen

George

1990

Plant Cell Tiss Organ Cult

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Cultured caryopses of finger millet (Eleusine coracana GAERTN) produced callus from shoot apices or mesocotyls depending upon the concentration of picloram and combination of cytokinins in MS basal medium. On subsequent subcultures, numerous somatic embryos differentiated from the callus on MS medium supplemented with picloram and kinetin. The embryos germinated into complete plants on medium devoid of phytohormones. When different carbohydrates were tested, basal medium containing glucose and sucrose produced the highest frequency of germinating somatic embryos. Supplementation of MS basal medium with a variety of aminoacids, osmotic agents and growth supplements had an adverse effect on the germination of embryos. Incorporation of different antibiotics such as carbenicillin, cefotaxime and streptomycin sulfate enhanced plant differentiation from somatic embryos. Cytological analysis of regenerated plants showed normal diploid chromosome number in their root tips.

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“…2,4-D was found to be the most effective source of auxin when used at 18.0 μM. The induction of callus in cereals and millets is commonly achieved by 2,4-D (Mohanty et al 1985;Chandra and Kothari 1995). However, in this study, addition of KN or ZN to medium containing 18.0 μM 2,4-D increased the percentage of somatic embryogenic induction from shoot apex explants, with the optimal response achieved on medium supplemented with KN at 2.3 μM.…”

Section: Discussionmentioning

confidence: 99%

“…Addition of plant growth regulators in the medium seems to be essential for efficient regeneration in finger millet. Mohanty et al (1985) used a lower level (1.1 μM) of 2,4-D for regeneration. KN and BAP have also been used for efficient shoot induction and plant regeneration in pearl millet (Mythili et al 2001;Srivastav and Kothari 2002), kodo millet (Nayak and Sen 1989), and foxtail millet (Xu et al 1984).…”

Section: Discussionmentioning

confidence: 99%

Efficient somatic embryogenesis and plant regeneration from shoot apex explants of different Indian genotypes of finger millet (Eleusine coracana (L.) Gaertn.)

Ceasar

Ignacimuthu²

2008

In Vitro Cell.Dev.Biol.-Plant

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An efficient somatic embryogenesis and plant regeneration system was developed from shoot apex explants of finger millet, Eleusine coracana. Eight genotypes, CO 7, CO 9, CO 13, CO 14, GPU 26, GPU 28, GPU 45, and GPU 48, were assessed in this study. The maximum somatic embryogenic induction, at 98.6%, was obtained from explants cultured on Murashige and Skoog medium supplemented with 18.0 μM dichlorophenoxyacetic acid and 2.3 μM kinetin. The highest number of shoot induction (26) was observed after transfer of embryonic callus to regeneration medium supplemented with 4.5 μM thidiazuran and 4.6 μM kinetin. Significant differences were observed between genotypes for somatic embryogenesis and plant regeneration. GPU 45 gave the best response, while CO 7 was the least responsive under the culture conditions tested in this study. Regenerated plants were successfully rooted and grown to maturity after hardening in soil.

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Callus initiation and plant regeneration in ragi (Eleusine coracana Gaertn.)

Cited by 30 publications

References 7 publications

Effects of cytokinins, carbohydrates and amino acids on induction and maturation of somatic embryos in kodo millet (Paspalum scorbiculatum Linn.)

Effects of cytokinins, carbohydrates and amino acids on induction and maturation of somatic embryos in kodo millet (Paspalum scorbiculatum Linn.)

Influence of phytohormones, carbohydrates, aminoacids, growth supplements and antibiotics on somatic embryogenesis and plant differentiation in finger millet

Efficient somatic embryogenesis and plant regeneration from shoot apex explants of different Indian genotypes of finger millet (Eleusine coracana (L.) Gaertn.)

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