Effects of cytokinins, carbohydrates and amino acids on induction and maturation of somatic embryos in kodo millet (Paspalum scorbiculatum Linn.)

Ceasar, S. Antony; Ignacimuthu, S.

doi:10.1007/s11240-010-9716-6

Cited by 38 publications

(12 citation statements)

References 48 publications

(39 reference statements)

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“…The successful use of shoot apical meristems explants in this study implies that they are a better choice for plant regeneration in cereals when compared to other explants. This study therefore confirms previous reports by Ramakrishnan et al (2014) and Ceasar and Ignacimuthu (2010). Mature seeds derived explants are better source material for tissue culture research than others because of the availability of seeds, ease of storage of seeds, and homogeneity quality of the explants (Yang et al, 2013).…”

Section: Discussionsupporting

confidence: 91%

Rapid and efficient plant regeneration from shoot apical meristems of finger millet [Eleusine coracana (L.) Gaertn.] via direct organogenesis

Mukami¹,

Ngetich²,

Mweu³

et al. 2018

Afr. J. Biotechnol.

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A simple and efficient plant regeneration system via direct organogenesis was established in finger millet using in vitro derived shoot apical meristems. Six varieties; GBK-043128, GBK-043094, GBK-043050, GBK-043137, GBK-043122 and GBK-043124 were evaluated. MS medium was used for cotyledonary germination. Maximum number of shoots (84.33%) was observed in variety GBK-043128 while GBK-043094 had the least germination efficiency (62. 67%). Shoot apical meristems from threeday old seedlings were evaluated for their potency of shoot induction on varied 6-benzylaminopurine (BAP) concentrations. Highest shoot induction was observed in medium supplemented with 1.75 mg/l BAP in GBK-043050 (3.00) whereas GBK-043094 (1.28) had the least response in medium supplemented with 1.0 mg/l BAP. To induce rooting, in vitro regenerated plants cultured on MS medium was supplemented with different concentrations of indole-3-acetic acid. The highest response in root induction, with a larger number of roots (10.28), was observed in MS medium supplemented with 4.0 µM IAA. Statistical analysis indicated that plant regeneration response varied greatly among the varieties. In vitro germinated plants were successfully transferred to the greenhouse after hardening, with 300 shoots developing into fertile plants, which were indistinguishable with wild type plants. This plant regeneration system has potential for production of transgenic finger millet crops.

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Section: Discussionsupporting

confidence: 91%

Rapid and efficient plant regeneration from shoot apical meristems of finger millet [Eleusine coracana (L.) Gaertn.] via direct organogenesis

Mukami¹,

Ngetich²,

Mweu³

et al. 2018

Afr. J. Biotechnol.

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“…Ashok Kumar et al (2000) also reported somatic embryos development from leaf explants on MS medium containing 2, 4-D in combination with BAP or Kin. Similar findings were also observed in case of kodo millet (Ceasar and Ignacimuthu 2010) and finger millet (Ceasar and Ignacimuthu 2008). Addition of BA in combination with 2,4-D to callus induction medium stimulated somatic embryogenesis and percentage of shoot-forming calli in Kentucky bluegrass (Poa pratensis L.) caryopsis culture (van der Valk et al 1995).…”

Section: Discussionsupporting

confidence: 83%

“…The induction of somatic embryogenesis has been achieved in several plant species by altering concentration and combination of auxins and cytokinins (Adda et al 1994;Ashok Kumar et al 2000;Ceasar and Ignacimuthu 2010), age of the cultures (Andrýskova et al 2009), osmotic stresses (Groll et al 2002;Zavattieri et al 2010), physiochemical factors (Hofmann et al 2004) and nitrogen sources (Sen et al 2002;Awal et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Influence of osmotic stress, physicochemical factors and nitrogen supplements on embryogenesis and plantlet formation in Guizotia abyssinica Cass. (Niger)

Ghane

Nikam

2014

Ind J Plant Physiol.

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An efficient protocol for somatic embryogenesis was developed for the edible oilseed crop Guizotia abyssinica Cass. (cv. IGP 76). Induction and proliferation of callus was achieved from cotyledonary explants on MS medium amended with 1.5 mg l -1 6-benzyladenine (BA) and 0.5 mg l -1 naphthalene acetic acid (NAA). Several auxin/cytokinin combinations, physical conditions, osmotic stresses and supplementary components, viz., amino acids and casein hydrolysate were tested for induction of embryogenic callus. Higher frequency of embryogenic calli (73.7 %) and somatic embryos per culture (22) were observed on MS ? 1.5 mg l -1 BA ? 0.5 mg l -1 NAA ? 0.5 % NaCl. Histological analyses showed typical globular and other stages of embryo development. The MS medium lacking growth regulators was best for the maximum germination of embryos (53.4 %) and formation of plantlets (65.4 %). Regenerated plantlets were acclimatized and transferred to the field with 62 % survival rate.

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“…It has been indicated that the ROS generated by inductive stresses can modify the effectiveness of microspore embryogenesis, influencing both cell viability and cell metabolism ( _ Zur et al 2009( _ Zur et al , 2014 and proline can function as non-enzymatic antioxidant by scavenging the ROS produced by inductive stresses and reestablish redox homeostasis (Szabados and Savouré 2009). The enhancing effect of proline on somatic embryogenesis induction has also been observed in several species (Ceasar and Ignacimuthu 2010;Nhu et al 2012). Patnaik et al (2005) applied polyethylene glycol (PEG-8000), mannitol and air drying treatment for somatic embryogenesis induction in T.aestivem L. Surprisingly, they observed twofold to fivefold increase in the endogenous level of proline content in embryogenic explants compared with non-embryogenic explants (untreated cultures).…”

Section: Discussionmentioning

confidence: 99%

“…Comparing the effects of three different amino acids i.e. proline, serine and glutamine on somatic embryogenesis in P. scorbiculatum L., Ceasar and Ignacimuthu (2010) noted that the MS medium containing 25 mg l -1 proline was superior to that containing serine and glutamine or untreated cultures in the term of somatic embryo formation and maturation. In addition, significant increase in the number of somatic embryos produced from in vitro cultured main root transverse thin cell layers of Panax vietnamensis was observed when 300 mg l -1 proline was exogenously applied to the induction medium (Nhu et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Proline and chitosan enhanced efficiency of microspore embryogenesis induction and plantlet regeneration in Brassica napus L.

Ahmadi

Shariatpanahi

2015

Plant Cell Tiss Organ Cult

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The effects of proline (0, 50, 100, 200 and 500 mg l -1 ) and chitosan (0, 10, 20, 50 and 100 mg l -1 ) treatment at three incubation periods (1, 2 and 5 days) were assessed on the efficiency of microsopore embryogenesis induction and subsequently plantlet regeneration in rapeseed (Brassica napus L.) cv. ''Hyola 401''. About 30-55 % increase in microspore embryogenesis was observed in the cultures exposed to 100 mg l -1 proline for 2 and 5 days. Higher levels were not advantageous so that, microspore embryogenesis significantly reduced when 200 and 500 mg l -1 proline were exogenously applied to the culture medium. High normal regeneration was achieved in the cultures treated with 50 and 100 mg l -1 proline for 2 and 5 days. In addition, callogenesis increased as proline level and duration of its treatment was increased in which the majority of microspore-derived embryos (78 %) underwent callusing in the cultures exposed to 500 mg l -1 proline for 5 days. In contrast to untreated cultures, the efficiency of microspore embryogenesis increased about 1.8-fold in response to 10 mg l -1 chitosan for 2 days. High levels of chitosan were detrimental to microspore embryogenesis so that embryogenesis was completely inhibited in the cultures exposed to 50 and 100 mg l -1 for 5 days. Chitosan treatment was not advantageous to the normal plantlet development at all levels and durations tested. High levels and durations of chitosan treatment resulted in the higher callusing so that the highest callusing (88 and 79 %) were observed in the presence of 100 mg l -1 for 1 day and 20 mg l -1 for 2 days, respectively. Efficiency of microspore embryogenesis induction and plantlet regeneration could be improved by proline and chitosan treatment when appropriate levels and durations of incubation were selected.

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Effects of cytokinins, carbohydrates and amino acids on induction and maturation of somatic embryos in kodo millet (Paspalum scorbiculatum Linn.)

Cited by 38 publications

References 48 publications

Rapid and efficient plant regeneration from shoot apical meristems of finger millet [Eleusine coracana (L.) Gaertn.] via direct organogenesis

Rapid and efficient plant regeneration from shoot apical meristems of finger millet [Eleusine coracana (L.) Gaertn.] via direct organogenesis

Influence of osmotic stress, physicochemical factors and nitrogen supplements on embryogenesis and plantlet formation in Guizotia abyssinica Cass. (Niger)

Proline and chitosan enhanced efficiency of microspore embryogenesis induction and plantlet regeneration in Brassica napus L.

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