Callus induction from leaves of differentpaulownia species and its plantlet regeneration

A Review of <i>Paulownia</i> Biotechnology: A Short Rotation, Fast Growing Multipurpose Bioenergy Tree

“…An efficient plant regeneration system (Figures 1(E) and (F)) is a prerequisite for transgenic plant production and further studies. Micropropagation protocols have been established for a number of Paulownia species ( Table 1) including P. catalpifolia [25,26], P. elongata [27,28], P. fortunei [29][30][31][32][33][34], P. kawakamii [35][36][37][38], P. taiwaniana [39][40][41] and P. tomentosa [29][30][31]37,38,[42][43][44][45]. Most protocols developed to date predominantly use nodal explants.…”

“…Callus induction was optimized in five Paulownia species (P. tomentosa, P. australis, P. fortunei, P. elongata, P. tomentosa × P. fortunei) using leaf explants [31]. MS medium [48] containing varying levels of NAA (0.1 -1.1 mg·L −1 ) and BAP (2 -12 mg·L −1 ) were tested for embryogenic culture induction and plant regeneration.…”

“…The technology assumes significance in terms of its utility for large-scale plant propagation in bioreactors, short-and long-term germplasm conservation, and easy transportation of planting stock material. Other reports on somatic embryogenesis and plant regeneration in P. elongata have been described [4,27,31,61], but have been difficult to replicate using stated procedures. Suspension cultures have great potential for screening and production of secondary metabolites in light of numerous medicinal phytochemicals occurring in Paulownia.…”