Callus induction and whole plant regeneration in elite Indian maize (Zea mays L.) inbreds

Abstract

Callus induction and regeneration ability of five elite maize inbred lines, CM 111, CM 117, CM 124, CM 125 and CM 300 were investigated using 14-day-old immature embryos as explants. Genotype, medium, source of auxin and their concentrations influenced induction of callus. Explants grown on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid at 1 mg l -1 showed the highest frequency of callusing. Among all the media tested, explants grown on N6 medium gave the highest frequency of organogenic callus. Moreover, N6 supplemented with Dicamba promoted higher callus response in terms of both frequency of induction as well as quality, compared to N6 medium with 2,4-D. N6 supplemented with 2 mg l -1 Dicamba induced the highest frequency of organogenic callus. Among the five genotypes tested, CM 124, CM 125, and CM 300 gave the best callus. Explants of both CM 124 and CM 300 incubated on MS medium supplemented with 1 mg l -1 benzyladenine and 0.5 mg l -1 indole acetic acid promoted the highest frequency of shoot induction. Though CM 124 induced higher percentage of shoot formation than CM 300, the mean number of developed shoots per explant was higher for CM 300. The highest frequency of root formation was observed when shoots were grown on MS medium supplemented with 2 mg l -1 naphathalene acetic acid. Percentage of regenerated plants ranged from 54 to 66.

Changes in tryptophan production level in maize callus through modification of (ASA2) gene for enhancing defense mechanism

“…Zygotic embryos variety 166 were dissected from ear and placed with scutellum side up on the callus induction medium containing 2 mg/l 2,4 -D. Immature embryos have been shown to be most efficient in their regeneration procedure. Induction of callus from immature embryos was found to be dependent on the presence of 2,4-D (Rakshit et al, 2010).…”

Induksi dan Regenerasi Kalus Jagung yang Ditransformasi dengan Gen CsNitr1-L melalui Penembakan Partikel

“…Keberhasilan proses transformasi tanaman sangat dipengaruhi oleh kemampuan atau daya regenerasi tanaman yang ditransformasi. Regenerasi tanaman jagung dapat dilakukan baik secara organogenesis (Danson et al, 2006;Rakshit et al, 2010) maupun embriogenesis somatik (Ombori et al, 2008). Jalur embriogenesis somatik memiliki beberapa tahapan proses perkembangan, yaitu pembentukan kalus, pembentukan embrio somatik, tahap pendewasaan, dan perkecambahan, sedangkan jalur organogenesis diawali dengan pembentukan organ secara langsung maupun tidak langsung yang melalui tahapan kalus.…”

“…Organogenesis (Danson et al, 2006;Rakshit et al, 2010) Embriogenesis (Ombori et al, 2008) Osmotikum dengan embrio somatik hijau, jumlah planlet, dan jumlah tanaman transgenik putatif.…”

Callus induction and plant regeneration from immature zygotic embryos of various maize genotypes (Zea mays L.)

“…옥수수의 미성숙 배, 성숙 배, 잎, 약 등 여러 다양한 기관이나 조직으로부터 식물체 재 분화에 대한 연구가 오랫동안 수행되어 왔다. 옥수수 미성 숙 배로부터 식물체 재분화가 Green and Philips (1975)에 의 해 처음 보고된 이후로 다양한 품종의 미성숙 배로부터 식 물체 재분화 연구가 지속적으로 보고되었다 (Armstrong and Green 1985;Duncan et al 1985;Songstad et al 1988;Bohorova et al 1995;Carvalho et al 1997;El-Itriby et al 2003;Wang et al 2007;Binott et al 2008;Ombori et al 2008;Rakshit et al 2010;Anami et al 2010;De-yi et al 2011;González et al 2012;Ali et al 2014). 미성숙 배 외에도 성숙 배 (Wang 1987;Huang and Wei 2004;Al-Abed et al 2006;Abebe et al 2008;Jia et al 2008;Zhao et al 2008), 중배축(mesocotyl) 조직 (Torne et al 1980), 약 (Ting et al 1981), 유묘의 마디부분 (Santos et al 1984;Sidorov et al 2006), 잎 (Conger et al 1987;Ahmadabadi et al 2007), 영 (glume) (Suprasanna et al 1986), 화서(미성숙 tassel과 ears) 절 단 조직 (Pareddy and Petolino 1990;Songstad et al 1992), shoot-tip 분열 조직 (Li et al 2004)에서 캘러스 형성 및 식물체 재분화가 이루어졌다.…”