Callus induction and plant regeneration from immature zygotic embryos of various maize genotypes (Zea mays L.)

Hong, Joon Ki; Park, Ki Jin; Lee, Gang-Seob; Kim, Dool Yi; Kim, Ju-Kon; Lee, Seung Bum; Suh, Eun Jung; Lee, Yeon-Hee

doi:10.5010/jpb.2017.44.1.049

Cited by 4 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the directions in the development of plant biotechnologies using calli is the identification of the relationship between the content of endogenous (in explant or callus) and the concentration of exogenous (in a culture medium) hormones for regulating morphogenesis pathways in vitro. Such studies are not numerous and they were carried mainly on cereals [12][13][14]. Based on the available literature, studies of endogenous hormones accumulation in order to identify their participation in the callusogenesis and the regulation of morphogenesis in callus cultures in vitro for lavender have not been previously conducted.…”

Section: Introductionmentioning

confidence: 99%

Induction of morphogenesis in the callus culture of Lavandula angustifolia Mill.

et al. 2020

View full text Add to dashboard Cite

The influence of the nutrient medium hormonal composition on the induction of calluso- and morphogenesis in Lavandula angustifolia Mill., as well as the content of some endogenous hormones (indolylacetic acid, cytokinins, abscisic acid) in morphogenic and non-morphogenic calli, were studied. The leaf explants were isolated from obtained in vitro plants of the Stepnaya and Vdala cultivars. In primary calli with a frequency of 5.7-11.6%, buds and shoots were formed on different Murashige and Skoog (MS) culture media supplemented with 6-benzylaminopurine, thidiazuron, kinetin, and gibberellic acid. In the callus of the first passage in both cultivars, the induction of morphogenesis was observed with the greatest frequency (39.5-43.2%) on the MS medium with the addition of 1.0 mg/l 6-benzylaminopurine and 1.0 mg/l kinetin. Morphogenic calli of the Vdala cultivar were characterized by a higher content of cytokinins, but a lower content of indolylacetic and abscisic acids compared with non-morphogenic ones.

show abstract

Section: Introductionmentioning

confidence: 99%

Induction of morphogenesis in the callus culture of Lavandula angustifolia Mill.

et al. 2020

View full text Add to dashboard Cite

show abstract

Cytophysiological Features of the Cereal-Based Experimental System “Embryo In Vivo–Callus In Vitro”

et al. 2021

View full text Add to dashboard Cite

A New Protocol to Mitigate Damage to Germination Caused by Black Layers in Maize (Zea mays L.) Seeds

Hong,

Baek,

Park

et al. 2023

Agriculture

View full text Add to dashboard Cite

Maize seeds harvested in the field have higher vitality than those harvested in greenhouses but have higher contamination rates in terms of fungal or bacterial infection. It is important to disinfect maize seeds before sowing because seeds are a source of infection and damage crop production. In this study, we aimed to provide an efficient seed sterilization method to manage fungal or bacterial infections of field-harvested maize seeds. The optimized sterilization protocol was set up according to the disinfectant liquid immersion time, inverting RPM (rotations per minute), number of seeds, and black layer removal. We put 20 grains of maize seeds in 100% commercial bleach disinfectant containing 4–5% NaClO and performed 20 min of inversion at 45 RPM. After standing without inverting for the next 25 min in the sterile hood, inversion at 45 RPM for another 40 min was performed. By using this protocol, microorganisms occurred at a low rate with an average of 11.7%. Moreover, it was shown that microorganisms occurred at the lowest rate (average of 0.29% of seeds) when the black layer was removed. In addition, this sterilization method did not affect the growth and development of maize plants. These results revealed that black layer removal from maize seeds is a highly efficient, easy, and inexpensive sterilization method and can be used for seeds of various maize lines.

show abstract

Callus induction and plant regeneration from immature zygotic embryos of various maize genotypes (Zea mays L.)

Cited by 4 publications

References 30 publications

Induction of morphogenesis in the callus culture of Lavandula angustifolia Mill.

Induction of morphogenesis in the callus culture of Lavandula angustifolia Mill.

Cytophysiological Features of the Cereal-Based Experimental System “Embryo In Vivo–Callus In Vitro”

A New Protocol to Mitigate Damage to Germination Caused by Black Layers in Maize (Zea mays L.) Seeds

Contact Info

Product

Resources

About