Callus induction and plant regeneration via leaf segments of three accessions of African rice (Oryza glaberrimaStued.)

Diawuoh, Rhoda Gyinae; Klu, G.Y.P.; Amoatey, H. M.; Otu, S.; Asare, Kwabena

doi:10.22161/ijeab/2.5.17

Cited by 2 publications

(3 citation statements)

References 7 publications

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“…Highly significant mean squares were noted for parameters which define tissue culture response over rice genotypes. Differential genotype response for callus induction has been reported by Diawuoh et al, (2016).…”

Section: Callus Initiationmentioning

confidence: 88%

“…Different concentrations of 2,4-D havebeen assessed by different workers to find the optimal working concentration that gives best callus induction. A study conducted by Vennapusa et al, (2015) showed that highest callus frequency was obtained on LS media containing 2.5 mg/L 2,4-D. Diawuoh et al, (2016) also worked with dehusked rice and reported that 2,4-D is the best auxin for callus induction.…”

Section: Callus Initiationmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation and Validation of Rice (Oryza sativa L.) Genotypes for Tissue Culture Response from Mature Embryos

Sakina¹,

Rather²,

Mir³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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Section: Callus Initiationmentioning

confidence: 88%

Section: Callus Initiationmentioning

confidence: 99%

Evaluation and Validation of Rice (Oryza sativa L.) Genotypes for Tissue Culture Response from Mature Embryos

Sakina¹,

Rather²,

Mir³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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“…This could be attributable to a lack of concern or interest in genetic manipulation, possibly due to the absence of the appropriate in vitro regeneration protocols for local cultivars. 20 , 21 In fact, difficulties in the transformation and regeneration of African local cultivars, such as O. glaberrima , have been reported in several studies. 20–23 …”

Section: Introductionmentioning

confidence: 99%

Transgenic rice Oryza glaberrima with higher CPD photolyase activity alleviates UVB-caused growth inhibition

2021

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The ultraviolet B (UVB) sensitivity of rice cultivated in Asia and Africa varies greatly, with African rice cultivars ( Oryza glaberrima Steud. and O. barthii A. Chev.) being more sensitive to UVB because of their low cyclobutane pyrimidine dimer (CPD) photolyase activity, which is a CPD repair enzyme, relative to Asian rice cultivars ( O. sativa L.). Hence, the production of UVB-resistant African rice with augmented CPD photolyase activity is of great importance, although difficulty in transforming the African rice cultivars to this end has been reported. Here, we successfully produced overexpressing transgenic African rice with higher CPD photolyase activity by modifying media conditions for callus induction and regeneration using the parental line (PL), UVB-sensitive African rice TOG12380 ( O. glaberrima ). The overexpressing transgenic African rice carried a single copy of the CPD photolyase enzyme, with a 4.4-fold higher level of CPD photolyase transcripts and 2.6-fold higher activity than its PL counterpart. When the plants were grown for 21 days in a growth chamber under visible radiation or with supplementary various UVB radiation, the overexpressing transgenic plants have a significantly increased UVB resistance index compared to PL plants. These results strongly suggest that CPD photolyase remains an essential factor for tolerating UVB radiation stress in African rice. As a result, African rice cultivars with overexpressed CPD photolyase may survive better in tropical areas more prone to UVB radiation stress, including Africa. Collectively, our results provide strong evidence that CPD photolyase is a useful biotechnological tool for reducing UVB-induced growth inhibition in African rice crops of O. glaberrima .

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Callus induction and plant regeneration via leaf segments of three accessions of African rice (Oryza glaberrimaStued.)

Abstract: Abstract-

Cited by 2 publications

References 7 publications

Evaluation and Validation of Rice (Oryza sativa L.) Genotypes for Tissue Culture Response from Mature Embryos

Evaluation and Validation of Rice (Oryza sativa L.) Genotypes for Tissue Culture Response from Mature Embryos

Transgenic rice Oryza glaberrima with higher CPD photolyase activity alleviates UVB-caused growth inhibition

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