Callus growth kinetics and accumulation of secondary metabolites of Bletilla striata Rchb.f. using a callus suspension culture

Abstract

Bletilla striata is an endangered traditional Chinese medicinal plant with multiple uses and a slow regeneration rate of its germplasm resources. To evaluate the callus growth kinetics and accumulation of secondary metabolites (SMs), a callus suspension culture was proven to be a valuable approach for acquiring high yields of medicinal compounds. An effective callus suspension culture for obtaining B. striata callus growth and its SMs was achieved with the in vitro induction of calluses from B. striata seeds. The callus growth kinetics and accumulation of SMs were analyzed using a mathematical model. The resulting callus growth kinetic model revealed that the growth curves of B. striata suspension-cultured calluses were sigmoidal, indicating changes in the growth of the suspension-cultured calluses. Improved Murashige and Skoog callus growth medium was the most favorable medium for B. striata callus formation, with the highest callus growth occurring during the stationary phase of the cultivation period. Callus growth acceleration started after 7 days and thereafter gradually decreased until day 24 of the cultivation period and reached its highest at day 36 period in both the dry weight and fresh weight analyses. The coelonin concentration peaked during the exponential growth stage and decreased afterward during the stationary stage of the callus suspension culture. The maximum content of coelonin (approximately 0.3323 mg/g callus dry weight) was observed on the 18th day of the cultivation cycle, while dactylorhin A and militarine reached the highest concentrations at day 24, and p-hydroxybenzyl alcohol at day 39. This investigation also laid a foundation for a multimathematical model to better describe the accumulation variation of SMs. The production of SMs showed great specificity during callus growth and development. This research provided a well-organized way to increase the accumulation and production of SMs during the scaled-up biosynthesis of calluses in B. striata callus suspension cultures.

Nanoparticles induce genetic, biochemical, and ultrastructure variations in Salvadora persica callus

“…The synthesis of secondary metabolites in plants is closely related to the growth stage and environmental conditions, and each type of secondary metabolites has different biosynthetic pathways at different stages of growth. In general, the secondary metabolite concentration peaked during the exponential growth stage and decreased afterward during the stationary stage of the callus culture [38]. The NPs can elicit oxidative damage in plants by generating reactive oxygen species (ROS), such as H 2 O 2 and hydroxyl radical, causing damage to lipid membranes, proteins, and nucleic acids, which results in denaturation of cellular components or activation of the defense system in plants [39,40].…”

Metabolic Profiling of Primary Metabolites and Galantamine Biosynthesis in Wounded Lycoris radiata Callus

“…This technology is one of the most important methods for the biosynthesis of various secondary metabolites from the callus. In various medicinal plants, the callus suspension culture system has been recognized throughout the world but the wounded L. radiata callus and analysis of secondary metabolites have not yet been studied [ 14 , 15 , 16 ]. Previously, few studies have been carried out to analyze the secondary metabolite content in the bulb, leaf, and root of L. radiata [ 4 ].…”

Full Transcriptome Analysis of Callus Suspension Culture System of Bletilla striata

“…The culture system was improved based on our former study (Pan et al, 2020). The loose and bright yellow callus obtained after two subculture processes for about 30 days were used as explants for suspension culturing and inoculating into 100 mL culture flask which containing 35 mL liquid medium.…”