Callus culture development of two varieties of Tagetes erecta and carotenoid production

Benítez-García, Israel; Vanegas‐Espinoza, Pablo Emilio; Meléndez-Martínez, Antonio J.; Heredia, Francisco J.; Paredes‐López, Octavio; Villar‐Martínez, Alma Angélica Del

doi:10.1016/j.ejbt.2014.01.004

Cited by 24 publications

(17 citation statements)

References 32 publications

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“…Examples are scattered in the literature with in vitro cultures producing compounds of interest either using dedifferentiated cells from callus or undifferentiated cells from meristematic cambial cells. From the calli, systems of plant suspension cell cultures can be generated like for example for acteoside production from Scrophularia stiata (Khanpour-Ardestani et al, 2015), rosmarinic acid from Satureja khuzistanica (Sahraroo et al, 2014), or carotenoid from Tagetes erecta (Benítez-García et al, 2014). On the other hand, tissue cultures (i.e.…”

Section: Lautié Et Al Maximizing Explored Plant Chemical Diversitymentioning

confidence: 99%

Unraveling Plant Natural Chemical Diversity for Drug Discovery Purposes

Lautie

Russo

Ducrot³

2020

Front. Pharmacol.

156

120

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The screening and testing of extracts against a variety of pharmacological targets in order to benefit from the immense natural chemical diversity is a concern in many laboratories worldwide. And several successes have been recorded in finding new actives in natural products, some of which have become new drugs or new sources of inspiration for drugs. But in view of the vast amount of research on the subject, it is surprising that not more drug candidates were found. In our view, it is fundamental to reflect upon the approaches of such drug discovery programs and the technical processes that are used, along with their inherent difficulties and biases. Based on an extensive survey of recent publications, we discuss the origin and the variety of natural chemical diversity as well as the strategies to having the potential to embrace this diversity. It seemed to us that some of the difficulties of the area could be related with the technical approaches that are used, so the present review begins with synthetizing some of the more used discovery strategies, exemplifying some key points, in order to address some of their limitations. It appears that one of the challenges of natural product-based drug discovery programs should be an easier access to renewable sources of plant-derived products. Maximizing the use of the data together with the exploration of chemical diversity while working on reasonable supply of natural product-based entities could be a way to answer this challenge. We suggested alternative ways to access and explore part of this chemical diversity with in vitro cultures. We also reinforced how important it was organizing and making available this worldwide knowledge in an "inventory" of natural products and their sources. And finally, we focused on strategies based on synthetic biology and syntheses that allow reaching industrial scale supply. Approaches based on the opportunities lying in untapped natural plant chemical diversity are also considered.

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Section: Lautié Et Al Maximizing Explored Plant Chemical Diversitymentioning

confidence: 99%

Unraveling Plant Natural Chemical Diversity for Drug Discovery Purposes

Lautie

Russo

Ducrot³

2020

Front. Pharmacol.

156

120

View full text Add to dashboard Cite

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“…Moreover, variation in callus induction on different concentrations of growth regulators depends on the morphogenetic response which varies according to spatial and temporal cell distributions, and on physiological and developmental stages. Even there were the nodules in compact callus of Tagetes erecta from 2,4 D effect in medium Benítez-García et al (2014) and Ming et al (2019).…”

Section: Texture Of Callusmentioning

confidence: 95%

Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus

et al. 2020

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Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plants, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aimed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment.

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“…The pigmentation of the callus developed from yellow (YF) and white flower (WF) varieties was different. Callus of YF had lutein and zeaxanthin, whereas in WF callus lutein, zeaxanthin, β-cryptoxanthin and β-carotene are the main pigments [198]. Hairy root clones/ cultures of Tagetes erecta L. exhibited significant variation in growth pattern and total thiophene contents in comparison to wild type [199] which can be used for producing thiophenes at large scale.…”

Section: Role Of Biotechnologymentioning

confidence: 98%

Marigold: From Mandap to Medicine and from Ornamentation to Remediation

Mir¹,

Ahanger²,

Agarwal³

2019

AJPS

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Importance of medicinal plants to health care has been great and herbal preparations are being produced at industrial scale particularly in developing countries. The plant products obtained have a long history of use in therapeutics, aromatherapy and food depending on the chemical constituents and their bioactivity. In the recent past, marigolds have received a great attention in scientific research, because of their multiple use and also the information available about their phytochemistry and bioactivity. Tagetes species commonly known as marigold is native to Mexico, being used for medicinal and ornamental purposes. The plant is useful due to its unique phytoconstituents for a range of diseases and disorders and is reportedly effective against piles, kidney troubles, muscular pain, ulcers and wound healing and the flowers are helpful in fever, stomach and liver complaints and also in eye diseases. In India, marigold is also extensively used on religious and social occasions such as in the beautification of mandaps and pooja places; offerings at temples; marriage decorations and landscape planning due to variable size and colour of its flower. Present review is an effort to bring together the different strategies developed for the growth and cultivation of marigold, its ecophysiological and remediation relevance under a variety of environmental conditions and possible allelopathic potential. It includes reports on pharmacological aspects like antibacterial, antifungal, larvicidal, hepatoprotective, insecticidal, mosquitocidal, nematicidal, wound healing, antioxidant, anticancer and antidiabetic properties/activity of Tagetes.

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Callus culture development of two varieties of Tagetes erecta and carotenoid production

Cited by 24 publications

References 32 publications

Unraveling Plant Natural Chemical Diversity for Drug Discovery Purposes

Unraveling Plant Natural Chemical Diversity for Drug Discovery Purposes

Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus

Marigold: From Mandap to Medicine and from Ornamentation to Remediation

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