Callus and suspension cultures for biomass production ofCynara cardunculus (Compositae)

Figueiredo, A. Cristina; Fevereiro, Pedro; Novais, J. M.; Salomé, M.; Pais, S.

doi:10.1007/bf01024569

Cited by 22 publications

(8 citation statements)

References 15 publications

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“…In all cases the specific growth rate (/~) estimated was 0.15-0.20 d 1. These values are within the range obtained for different species reported by other authors: 0.144 d-1 for Silybum marianum [3], 0.314d 1 for Nicotiana tabacum [21], and 0.122 d i for Cynara cardunculus [4].…”

Section: Methodssupporting

confidence: 75%

Effect of carbon and nitrogen sources on growth and solasodine production in batch suspension cultures of Solanum eleagnifolium Cav.

Nigra

Álvarez

Giulietti

1990

Plant Cell Tiss Organ Cult

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The effect of inoculum size, carbon sources (fructose, glucose, maltose, sucrose), nitrate and ammonia on solasodine production by Solanum eleagnifolium Cav. was studied. The specific growth rate was estimated to be 0.15-0.20 d -l with all sugars tested at a concentration of 90 mM. Sucrose (180 mM) produced the highest biomass value (about 2.8 mgDWml 1) while the lowest one was produced by maltose. Although solasodine productivity values after 11 days of culture were similar for all sugars tested, the maximum values of productivity (0.9 mg g-ld-~) were achieved after 6 days of culture with sucrose (180 mM). Solasodine productivity of cultures conducted with a large inoculum (20% w/v fresh material) was double that with a small inoculum (10% w/v fresh material).

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Section: Methodssupporting

confidence: 75%

Effect of carbon and nitrogen sources on growth and solasodine production in batch suspension cultures of Solanum eleagnifolium Cav.

Nigra

Álvarez

Giulietti

1990

Plant Cell Tiss Organ Cult

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“…Zebu ( Bos indicus) pepsin extract was obtained by soaking the abomasum of adult animals (3 to 7 years old) in 6% NaCl and 0.1% sodium benzoate (Zalazar and Reinheimer 1980). O. gore tree bark was ground, dried at 50°C for 3h and disinfected with ethanol and then sodium hypochlorite (Figueiredo et al 1987) . The disinfected powder (75 g) was mixed with a solution of 3g CaCl 2 and 0.1 g sodium benzoate in 100 ml water and kept at 4°C for 24 h with occasional shaking.…”

Section: Methodsmentioning

confidence: 99%

Edibility of cheese manufactured using Ongokea gore extract

Libouga

2004

Tropical Science

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“…In previous studies, researchers assessed various auxin and cytokinin combinations in different media for callus formation in several plants. Regarding artichoke callus formation, Gamborg B5 medium with BA + 2,4-D (1:10) was found to be best (Figueiredo et al, 1987;Cimino et al, 2006). In another study, it was reported that 2,4-D was effective regarding both cell development and anthocyanin biosynthesis in callus cultures of Hibiscus sabdariffa plants (Mizukami et al, 1988;Siatka, 2019).…”

Section: Introductionmentioning

confidence: 93%

Callogenesis optimization of some globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] cultivars based on in vivo and in vitro leaf explants

Özsan

Onus

2020

Not Bot Horti Agrobo

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Globe artichoke’s [Cynara cardunculus var. scolymus (L.) Fiori] leaves are rich in polyphenols and due to health-promoting properties artichoke growing has been gaining interest. Optimization and development of valuable bioactive components, which are not in the standard amount in raw material can be achieved and increased with the assistance of in vitro techniques such as callus and subsequently cell suspension cultures. Therefore, in the present study in vitro callogenesis optimization of three globe artichoke cultivars was studied by using 29 different media combinations, based on basic Gamborg B5 medium supplemented with various concentrations of 1-Naphthaleneacetic acid (NAA), 6-Benzylaminopurine (BAP), 2,4-Dichlorophenoxyacetic acid (2,4-D), and Kinetin. Comparisons were made on the basis of using in vivo and in vitro leaves as explant material. In the experiment several parameters such as leaf explants development (%), callus formation (%), and callus weight (g) were assessed for each related cultivar. Results revealed that having auxin: cytokinin concentrations together at enough and well-balanced, having equal amounts or 10:1 concentrations of auxin: cytokinin, concentrations in media combinations are indispensable for stimulating the callogenesis in globe artichoke. The findings of the present study clearly revealed that, there were differences among cultivars regarding callus induction by using in vivo and in vitro leaf explants while in vivo leaf explants came into prominence regarding callus formation and weights. It is assumed that the findings of the present study may play a complementary and auxiliary role in several areas such as pharmaceutical engineering of globe artichoke.

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Callus and suspension cultures for biomass production ofCynara cardunculus (Compositae)

Cited by 22 publications

References 15 publications

Effect of carbon and nitrogen sources on growth and solasodine production in batch suspension cultures of Solanum eleagnifolium Cav.

Effect of carbon and nitrogen sources on growth and solasodine production in batch suspension cultures of Solanum eleagnifolium Cav.

Edibility of cheese manufactured using Ongokea gore extract

Callogenesis optimization of some globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] cultivars based on in vivo and in vitro leaf explants

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