Callus and etiolation induction data from explants of Solanecio biafrae (Olive &amp; Hierne) C. Jeffrey cultured in the dark

Bello, Oluwakemi A.; Fajimi, O; Esan, E. B.; Obembe, Olawole O.

doi:10.1016/j.dib.2018.07.029

Cited by 3 publications

(2 citation statements)

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“…Nodal segments were thoroughly cleaned under running tap water. They were surface-disinfected according to Bello 7 and the disinfectants were washed off using sterile distilled water (SDW).…”

Section: Explant Preparationmentioning

confidence: 99%

In vitro Regeneration of Solanecio biafrae Through Direct Shoot Organogenesis

2021

TJNPR

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Solanecio biafrae (Olive & Hierne) C. Jeffrey (Asteraceae) is a tropical, underutilized African indigenous vegetable, highly nutritious and medicinal. It grows as undercover and is mostly collected in tree plantations such as cocoa and kola. Its propagation is seldom carried out by vines, but its climbing habit poses a problem for farmers, which leads to the application of herbicide to control their spread. In order to ascertain its potential for genetic improvement, the protocol for direct shoot organogenesis of S. biafrae was developed. Leaf lamina, petiole, and leaf with petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with varying concentrations of 6-benzylaminopurine (BAP) alone and in combination with Indole butyric acid (IBA). Direct shoot organogenesis was obtained from the cut end of leaf lamina and petiole explants as well from the petiolar region of the leaf with petiole explant. The results obtained from this study show the potential of these explants of S. biafrae to serve as culture material for its micropropagation, genetic improvement via transgenesis. Hence, this regeneration protocol of S. biafrae can be coupled to its genetic transformation to improve species with shorter internode and more leaves to enhance the structure of tropical vegetation.

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Section: Explant Preparationmentioning

confidence: 99%

In vitro Regeneration of Solanecio biafrae Through Direct Shoot Organogenesis

2021

TJNPR

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“…Furthermore, callus formation is important to seal wounds, prevent water loss and provide cellular sources for vasculature differentiation (Ikeuchi et al 2017). Incubating various types of explants on auxin-rich callus-inducing media (CIM) can induce callus formation (Dale and Deambrogio 1979;Ikeuchi et al 2013;Bello et al 2018;Shin and Seo 2018). Callus induction occurs when plant cells dedifferentiate and proliferate.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Genetic analysis of callus formation in a diversity panel of 96 rose genotypes

Nguyen

Winkelmann

Debener

2020

Plant Cell Tiss Organ Cult

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In a diversity panel of 96 rose genotypes, variation in the capacity to form calluses on leaf explants in vitro was investigated, and a genome-wide association study (GWAS) was performed to identify genetic factors associated with callus formation. Calluses were induced from wounded in vitro leaflets on two media differing in their plant growth regulator composition. Significant differences between genotypes were observed in callus size on the first callus-inducing medium (CIM1, containing 10.7 µM naphthylene acetic acid) using a 0-4 scale, as well as on a second callus-inducing medium (CIM2, containing 4.5 µM dichlorophenoxyacetic acid and 2 µM 6-(γ,γ-dimethylallylaminopurine)) with callus size scales of 0.82-4. GWAS utilizing the WagRhSNP 68K SNP array for callus size induced on either CIM1 or CIM2 enabled the identification of 26 and 13 significantly associated SNPs, respectively. Among these SNPs, we found the SNPs Rh12GR_12098_1092Q (uncharacterized gene) and RhMCRND_2903_1233Q in a gene encoding a pentatricopeptide repeat-containing protein were associated with callus size on CIM1, with large effects being observed between alleles. Two SNPs, RhK5_5473_763P (S-formylglutathione hydrolase) and Rh12GR_37799_568Q (polyglutamine binding protein, WW domain binding protein), were associated with callus size on CIM2 with large effect sizes. The markers associated with callus size on CIM1 form a large cluster on chromosome 3 and minor clusters on other chromosomes and provide the first preliminary indications of candidate genes responsible for the observed phenotypic variation. Key message Callus formation in rose is strongly genotype dependent and varies continuously among 96 genotypes. A major QTL associated to callus size was located on chromosome 3.

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Optimization of Explant Surface Sterilization Conditions and Multiple Shoot Induction in Threatened Plant Phanera sirindhorniae

Sirimat¹,

Sakulsathaporn²

2019

JOAAT

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Callus and etiolation induction data from explants of Solanecio biafrae (Olive & Hierne) C. Jeffrey cultured in the dark

Cited by 3 publications

References 1 publication

In vitro Regeneration of Solanecio biafrae Through Direct Shoot Organogenesis

In vitro Regeneration of Solanecio biafrae Through Direct Shoot Organogenesis

Genetic analysis of callus formation in a diversity panel of 96 rose genotypes

Optimization of Explant Surface Sterilization Conditions and Multiple Shoot Induction in Threatened Plant Phanera sirindhorniae

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