Callose accumulation in roots of soybean seedlings under water deficit

Ehrhardt-Brocardo, Natalia Carolina Moraes; Coelho, Cileide Maria Medeiros; Souza, Clóvis Arruda; Mathias, Vanderléia

doi:10.1007/s40626-019-00160-y

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…In our study, we showed that mannitol‐induced osmotic stress causes callose deposition in both shoots and roots of soybean seedlings. It has already been shown that the accumulation of callose in soybean roots under water stress may be indicative of susceptibility to water stress (Ehrhardt‐Brocardo et al, 2019). In Arabidopsis, the importance of callose deposition for the tolerance of seeds and roots to osmotic stress has already been reported (Hunter et al, 2019; Pillai et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Flagellin and mannitol modulate callose biosynthesis and deposition in soybean seedlings

Sangi

Olimpio

Coelho

et al. 2023

Physiologia Plantarum

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Callose is a polymer deposited on the cell wall and is necessary for plant growth and development. Callose is synthesized by genes from the glucan synthase‐like family (GSL) and dynamically responds to various types of stress. Callose can inhibit pathogenic infection, in the case of biotic stresses, and maintain cell turgor and stiffen the plant cell wall in abiotic stresses. Here, we report the identification of 23 GSL genes (GmGSL) in the soybean genome. We performed phylogenetic analyses, gene structure prediction, duplication patterns, and expression profiles on several RNA‐Seq libraries. Our analyses show that WGD/Segmental duplication contributed to expanding this gene family in soybean. Next, we analyzed the callose responses in soybean under abiotic and biotic stresses. The data show that callose is induced by both osmotic stress and flagellin 22 (flg22) and is related to the activity of β‐1,3‐glucanases. By using RT‐qPCR, we evaluated the expression of GSL genes during the treatment of soybean roots with mannitol and flg22. The GmGSL23 gene was upregulated in seedlings treated with osmotic stress or flg22, showing the essential role of this gene in the soybean defense response to pathogenic organisms and osmotic stress. Our results provide an important understanding of the role of callose deposition and regulation of GSL genes in response to osmotic stress and flg22 infection in soybean seedlings.

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Section: Discussionmentioning

confidence: 99%

Flagellin and mannitol modulate callose biosynthesis and deposition in soybean seedlings

Sangi

Olimpio

Coelho

et al. 2023

Physiologia Plantarum

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“…After depositing a metal film, slicing and double staining, transmission electron microscopy was used for observation and photography (JME‐1200Ex; Japan Electronics Co.). Callose accumulation was observed according to the method of Ehrhardt‐Brocardo et al (2019). A piece of epidermis (1 × 0.5 cm) was peeled off for observation at 400× using a fluorescence microscope.…”

Section: Methodsmentioning

confidence: 99%

Changes in biochemistry and cellular ultrastructure support different resistance mechanisms to Phytophthora sojae in nonhost common bean and host soybean

Song

et al. 2022

Plant Pathology

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Phytophthora root and stem rot (PRR) of soybean (Glycine max) caused by Phytophthora sojae is a serious disease that affects soybean production, causing a global economic loss of approximately $1-2 billion every year (Tyler, 2007). P. sojae is a typical soilborne oomycete pathogen with oospores that survive in the soil for several years. When conditions are favourable, oospores germinate to produce sporangia that release the core infection structures, zoospores (Schmitthenner, 1985). P. sojae can infect soybean at any growth stage, resulting in seed, root and stem rot and plant death (Schmitthenner, 1985). The rational use of soybean resistance is the most economical and effective means to control PRR (Zhang et al., 2013).The relationship between host soybean and P. sojae conforms to the gene-for-gene model, in which the proteins of Rps genes in soybean recognize those encoded by the corresponding Avr genes in P. sojae, which determines the resistance of soybean against P. sojae (Schmitthenner, 1985). More than 28 genes involved in resistance against P. sojae have been reported in soybean plants (Zhang et al.,

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Localization of seed-derived and externally supplied nutrients in peanut seedling root

Acharya

Pesacreta

2021

Theor. Exp. Plant Physiol.

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Callose accumulation in roots of soybean seedlings under water deficit

Cited by 4 publications

References 25 publications

Flagellin and mannitol modulate callose biosynthesis and deposition in soybean seedlings

Flagellin and mannitol modulate callose biosynthesis and deposition in soybean seedlings

Changes in biochemistry and cellular ultrastructure support different resistance mechanisms to Phytophthora sojae in nonhost common bean and host soybean

Localization of seed-derived and externally supplied nutrients in peanut seedling root

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