Calli and suspension cultures for biomass production ofEuphorbia characias L. subsp.characias

Strategy for early callus induction and identification of anti-snake venom triterpenoids from plant extracts and suspension culture of Euphorbia hirta L.

“…Likewise, the proliferation rate and callus initiation from pretreated explants showed higher rate than the untreated leaf explants after 3 week interval. This strategy might be useful in downstream applications and in large-scale bioreactors for producing bioactive compounds at a much faster rate (Fernandes-Ferreira et al 1989). There are also some studies, which have shown that exogenous uptake of phytohormones has a positive effect on the development of callus (Štefančič et al 2007).…”

“…In this study, the growth curve of E. hirta cell suspension cultures in MS medium supplemented with 1 mg L −1 and 2 mg L −1 of NAA and BAP has showed that the initial lag phase (4 days) period and entered growth phase (16th day) which continued by exhibiting the optimum concentration at 2 mg L −1 of NAA and BAP. Similarly, a typical growth curve of suspension cultures of E. characias was maintained on MS medium supplemented with 2, 4-D and BAP which revealed the initial lag phase for 7 days and exponential phase for 14 days (Fernandes-Ferreira et al 1989). Among the various combinations of growth regulators tested (2,4-D and Kin; NAA and Kin; BAP and NAA), MS medium supplemented with BAP (8.88 μM) and NAA (5.37 μM) induced maximum callus formation (Saad et al 2018).…”