Somatic embryogenesis, organogenesis and callus growth kinetics of flax

Cunha, Ana; Ferreira, Manuel

doi:10.1007/bf02318959

Cited by 28 publications

(14 citation statements)

References 26 publications

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“…Unlike other species, the induction of flax calli seems to be independent from the type of primary explant as well as shoot and root regeneration of L. usitatissimum seems to be possible from any green part of the seedling (Gomes da Cunha & Fereira, 1996). In contrast to this, Dedicova et al (2000) observed that cotyledons cultured in vitro do not form shoot initials under any of the culture media and conditions tested, a result similar to one obtained by Bretagne et al (1994).…”

Section: The Influence Of the Initial Explantsupporting

confidence: 56%

“…Flax is susceptible to many tissue culture techniques, which have been applied for more than 20 years (Pretova, 1974;Rybczynski, 1975;Gamborg and Shyluk, 1976;Murray et al, 1977;Lane, 1979;McHughen and Schwartz, 1984;Pretova and Williams, 1986;Nichterlein and Friedt, 1993;Gomes da Cunha & Fereira, 1996;Bergmann and Friedt, 1997;Dedicova et al, 2000;Tejavathi et al, 2000). Nevertheless, the knowledge about factors controlling the organogenesis and embryogenesis in flax is still insufficient.…”

Section: Tissue Culture Of Flaxmentioning

confidence: 99%

See 1 more Smart Citation

Flax Improvement by Biotechnology Means

Evtimova

Vlahova

Atanassov

2005

Journal of Natural Fibers

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The present paper is a short review of publications revealing development and optimization in tissue culture techniques (regeneration via organogenesis, somatic embryogenesis, anther cultures) and elaboration of efficient protocols for gene transfer as a tool for flax improvement. Different factors affecting the processes of in vitro regeneration and transformation are outlined. Perspectives in the area of biotechnological improvement of flax are discussed.

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Section: The Influence Of the Initial Explantsupporting

confidence: 56%

Section: Tissue Culture Of Flaxmentioning

confidence: 99%

Flax Improvement by Biotechnology Means

Evtimova

Vlahova

Atanassov

2005

Journal of Natural Fibers

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“…It also has been shown for flax that the production of somatic embryos is improved when exposure of the explants to the auxin is reduced (Gomes da Cunha and Fernandes-Ferrereira, 1996). The typical developmental stages were observed in somatic embryos developing from the different explants when cultured in MSI medium supplemented with kinetin and then transferred to MSII medium.…”

Section: Resultsmentioning

confidence: 94%

“…Auxins (Gomes da Cunha and Fernandes-Ferrereira, 1996;Jeya Mary and Jayabalan, 1997;Yu et al, 1997) and cytokinins (Gomes da Cunha and Fernandes-Ferrereira, 1996;Hita et al, 1997;Jeya Mary and Jayabalan, 1997) are necessary for the induction of somatic embryogenesis in diverse species.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration in Medicago arborea L.

Gallego

Hita

Villalobos

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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An efficient plant regeneration system employing cotyledons, hypocotyls, petioles and leaves as explants and characterized by continuous and prolific production of somatic embryos, has been developed with Medicago arborea ssp. arborea. The optimal somatic embryogenic response was obtained using a two-step protocol, where explants were incubated under a 16 h photoperiod for 2 mo. on Murashige and Skoog (MS) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D; 9 mM) and kinetin (9 mM), and followed by transfer to kinetin-free MS medium with 2,4-D (2.25 mM). Removal of the cytokinin and a reduction in the concentration of auxin (2.25 mM) in the second step of culture were critical for enhanced production of somatic embryos. The best explants proved to be cotyledons and petioles (i.e. a mean of 18X0^0X70 somatic embryos at 3 mo. for petiole culture). Somatic embryos were converted into normal plantlets 8X00 X89% when cultured on basal MS medium with 5 mM indolebutyric acid. No somatic embryos were obtained when thidiazuron was used in the culture media. Using petioles as explants and N 6 -benzyladenine (BA), embryogenesis was induced in the second step of culture when BA was removed from the medium and the concentration of 2,4-D was decreased to 2.25 mM.

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“…In the present study, both shoot induction and callus formation were induced from the in vitro stem explants of L. pumila cultured in medium containing zeatin. Cunha and Ferreira (1996) also concluded that several auxins and zeatin promoted the growth of Euphorbia characias calli.…”

Section: Callus Induction From In Vitro Leaf and Stem Explantsmentioning

confidence: 96%

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

Ling

Tan

Hussein

2013

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs.

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Somatic embryogenesis, organogenesis and callus growth kinetics of flax

Cited by 28 publications

References 26 publications

Flax Improvement by Biotechnology Means

Flax Improvement by Biotechnology Means

Somatic embryogenesis and plant regeneration in Medicago arborea L.

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

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