Calibration of Trapping Force on Cell-Size Objects From Ultrahigh-Frequency Single-Beam Acoustic Tweezer

Lim, Hae Gyun; Liu, Ying; Lin, Ming-Yi; Yoon, Changhan; Lee, Changyang; Jung, Hayong; Chow, Robert H.; Shung, K. Kirk

doi:10.1109/tuffc.2016.2600748

Cited by 31 publications

(33 citation statements)

References 37 publications

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“…23 The micropipette was upgraded by reducing the size of inner diameter into 0.5 μm. The main advantage of this technique is its capability of measuring the trapping force ranging from pN to nN depending on transducer’s driving conditions.…”

Section: Methodsmentioning

confidence: 99%

“…Stronger trapping forces in a range from pN to nN depending on the driving conditions 17,18,22,23 2. There are no mechanical contact and no labeling, thus less likely to lead to damages on objects on which measurements are to be performed 3.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, a calibration system based on MAT for the trapping force of the SBAT at UHF was recently developed. 23 The 410 MHz SBAT was used to trap and detach RBC from a RBC aggregate. The intercellular adhesion forces between two RBCs were estimated against the pre-calibrated trapping force using a MAT.…”

Section: Introductionmentioning

confidence: 99%

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Quantification of Inter-Erythrocyte Forces with Ultra-High Frequency (410 MHz) Single Beam Acoustic Tweezer

Lim

Shung

2017

Ann Biomed Eng

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Efforts on quantitative measurements of the interactive forces of red blood cells (RBC) have been pursued for many years in hopes of a better understanding of hemodynamics and blood rheology. In this paper, we report an approach based on an ultra-high frequency (410 MHz) single beam acoustic tweezer (SBAT) for quantitative measurements of inter-RBC forces at a single cell level. The trapping forces produced by this ultra-high frequency (UHF) SBAT can be quantitatively estimated with a micropipette. Since the focal beam diameter of the 410 MHz ultrasonic transducer used in this SBAT was only 6.5 micrometer (μm), which was smaller than that of a RBC (~7.5 μm), it was made possible to directly apply the beam to a single RBC and measure inter-RBC forces against the pre-calibrated acoustic trapping forces as another example of potential cellular applications of the SBAT. The magnitude of these forces was found to be 391.0 ± 86.4 pN. Finally, it is worth noting that unlike several other methods, this method does not require the measuring device to be in contact with the cells.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantification of Inter-Erythrocyte Forces with Ultra-High Frequency (410 MHz) Single Beam Acoustic Tweezer

Lim

Shung

2017

Ann Biomed Eng

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“…Examples include cell synchronization [3], the enrichment of prostate cancer cells in blood [4], the manipulation of C. elegans [5], and single-cell patterning [6]. Acoustics can also be used for noncontact microfluidic trapping and particle enrichment [7][8][9], as well as acoustic tweezing [10][11][12][13][14]. Trapping, an important unit operation in sophisticated celland bioparticle-handling systems, can also be obtained using other technologies, such as those in hydrodynamic [15], electro-and dielectrophoretic [16], and opticaltrapping systems [17]; see the review by Nilsson et al [18].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Numerical Modeling of Acoustic Trapping in Glass Capillaries

Ley

Bruus

2017

Phys. Rev. Applied

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Acoustic traps are used to capture and handle suspended microparticles and cells in microfluidic applications. A particular simple and much-used acoustic trap consists of a commercially available, millimeter-sized, liquid-filled straight glass capillary actuated by a piezoelectric transducer. Here, we present a three-dimensional numerical model of the acoustic pressure field in the liquid coupled to the displacement field of the glass wall, taking into account mixed standing and traveling waves as well as absorption. The model explains the dynamical mechanism that leads to the formation of localized acoustic resonance modes in such a straight acoustic waveguide without any geometrical cavities in the axial direction of the capillary. The model further predicts that some of these modes are well suited for acoustic trapping, and it provides estimates for their frequencies and quality factors, the magnitude of the acoustic radiation force on a single test particle as a function of position, and the resulting acoustic retention force of the trap. We show that the model predictions are in agreement with published experimental results, and we discuss how improved and more-stable acoustic-trapping modes might be obtained using the model as a design tool.

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“…A 230‐MHz self‐focused AlScN film transducer can be used to trap 10μm microspheres and epidermoid carcinoma cell (Zhu et al, ). Besides for trapping and manipulating smaller particles, acoustic trapping force calibration of UHF SBAT is another useful approach to evaluate the trapping performance of SBAT (Lim et al, ). However, there is less work reported for fully studying the trapping capability of one transducer for microparticles with different size, and systematically exploring the relationships between ultrasound frequency, beam width, and particle size.…”

Section: Introductionmentioning

confidence: 99%

An adjustable multi‐scale single beam acoustic tweezers based on ultrahigh frequency ultrasonic transducer

Chen

Lam

Chen

et al. 2017

Biotech & Bioengineering

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This paper reports the fabrication, characterization, and microparticle manipulation capability of an adjustable multi-scale single beam acoustic tweezers (SBAT) that is capable of flexibly changing the size of "tweezers" like ordinary metal tweezers with a single-element ultrahigh frequency (UHF) ultrasonic transducer. The measured resonant frequency of the developed transducer at 526 MHz is the highest frequency of piezoelectric single crystal based ultrasonic transducers ever reported. This focused UHF ultrasonic transducer exhibits a wide bandwidth (95.5% at -10 dB) due to high attenuation of high-frequency ultrasound wave, which allows the SBAT effectively excite with a wide range of excitation frequency from 150 to 400 MHz by using the "piezoelectric actuator" model. Through controlling the excitation frequency, the wavelength of ultrasound emitted from the SBAT can be changed to selectively manipulate a single microparticle of different sizes (3-100 μm) by using only one transducer. This concept of flexibly changing "tweezers" size is firstly introduced into the study of SBAT. At the same time, it was found that this incident ultrasound wavelength play an important role in lateral trapping and manipulation for microparticle of different sizes. Biotechnol. Bioeng. 2017;114: 2637-2647. © 2017 Wiley Periodicals, Inc.

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Calibration of Trapping Force on Cell-Size Objects From Ultrahigh-Frequency Single-Beam Acoustic Tweezer

Cited by 31 publications

References 37 publications

Quantification of Inter-Erythrocyte Forces with Ultra-High Frequency (410 MHz) Single Beam Acoustic Tweezer

Quantification of Inter-Erythrocyte Forces with Ultra-High Frequency (410 MHz) Single Beam Acoustic Tweezer

Three-Dimensional Numerical Modeling of Acoustic Trapping in Glass Capillaries

An adjustable multi‐scale single beam acoustic tweezers based on ultrahigh frequency ultrasonic transducer

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