Amid growing concerns surrounding the longevity of individuals,
the acceleration of aging by pollutants has garnered significant attention.
Tris(2-butoxyethyl) phosphate (TBOEP), a widely used organophosphorus
flame retardant (OPFR), has been frequently detected in the environment;
however, its impact on organism life spans remains unclear. This study
investigated the effects of TBOEP on the life span of Caenorhabditis
elegans at environmentally relevant concentrations of 0,
50, 500, and 5000 ng/L. TBOEP exposure reduced motility and shortened
the life span of C. elegans in a dose-dependent manner,
with the most significant effects observed at 5000 ng/L. Transcriptomics
and metabolomics revealed that 5000 ng/L TBOEP significantly disrupted
longevity regulation in C. elegans. This was mainly
attributed to the abnormal regulation of genes encoding the heat shock
proteins, lipase, and stearoyl-CoA desaturase as well as the increased l-arginine content in C. elegans. Concurrently,
exposure to 5000 ng/L TBOEP significantly upregulated the microRNAs
in the lethal-7 family, thereby accelerating C. elegans senescence. Overall, this study provides important
insights into the effects of TBOEP on C. elegans senescence
and could inform further studies assessing the environmental health
risks of OPFRs.