Calf Spleen Extractive Injection (CSEI), a small peptides enriched extraction, induces human hepatocellular carcinoma cell apoptosis via ROS/MAPKs dependent mitochondrial pathway

Jia, Dongxu; Lü, Wei; Zhang, Xinrui; Cai, Guangsheng; Teng, Lirong; Wang, Xinyu; Zhang, Minghai; Zeng, Yan; Liang, Chunhua; Wang, Di

doi:10.1016/j.jphs.2016.08.006

Cited by 11 publications

(5 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…e reason may be that lienal polypeptide injection enters the spleen with blood circulation through an intravenous drip and improves immune cell activity through bidirectional multitarget immunomodulation, which enhances cellular nonspecific immune response and prevents serious toxic side effects. Previous research [20] suggests that lienal polypeptide injection has been widely used in postoperative chemotherapy for various cancers such as liver cancer and breast cancer with certain effectiveness, and the results of the present study also demonstrated its ability to mitigate the irritation of chemotherapy drugs on patients' gastrointestinal tract and effectively improve body immunity. Moreover, the observation group showed a significantly higher treatment efficacy and a lower incidence of adverse events than the control group.…”

Section: Discussionsupporting

confidence: 66%

Clinical Efficacy of Laparoscopic Billroth II Subtotal Gastrectomy Plus Lienal Polypeptide Injection for Gastric Cancer

Yan

2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Objective. To evaluate the clinical efficacy of laparoscopic Billroth II subtotal gastrectomy plus lienal polypeptide injection for gastric cancer. Methods. Between May 2018 and January 2021, 110 patients with gastric cancer treated in Jingzhou First People’s Hospital were recruited and assigned via the random number table method to either an observation group or a control group, with 55 patients in each group. All patients received laparoscopic Billroth II subtotal gastrectomy, and the observation group additionally received lienal polypeptide injection. Outcome measures include surgical indexes, clinical efficacy, and adverse events. Results. The patients in the observation group had significantly less intraoperative hemorrhage volume, smaller surgical wounds, shorter time lapse before passing gas and hospital stay, and longer operation time than those in the control group ( P < 0.001 ). The observation group showed significantly higher efficacy than the control group ( P = 0.001 ). The observation group had a significantly lower incidence of toxic side effects and adverse events than the control group ( P < 0.05 ). After treatment, the CD3+ and CD4+ levels were significantly elevated and the CD8+ level was decreased, with higher CD3+ and CD4+ levels and lower CD8+ levels in the observation group than in the control group ( P < 0.05 ). Conclusion. In the treatment of patients with gastric cancer, laparoscopic Billroth II subtotal gastrectomy plus lienal polypeptide injection features promising efficacy, improves the immune function of patients, effectively reduces the occurrence of toxic side effects and adverse reactions, with less trauma and rapid recovery, which shows good potential for use in clinical application.

show abstract

Section: Discussionsupporting

confidence: 66%

Clinical Efficacy of Laparoscopic Billroth II Subtotal Gastrectomy Plus Lienal Polypeptide Injection for Gastric Cancer

Yan

2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…Extensive reports showed that ROS was closely related to JNK, P38 and ERKs pathway [47, 48]. These findings are consistent with our results.…”

Section: Discussionsupporting

confidence: 93%

Astaxanthin mitigates cobalt cytotoxicity in the MG-63 cells by modulating the oxidative stress

Da-he

Tong

Liu

et al. 2017

BMC Pharmacol Toxicol

View full text Add to dashboard Cite

BackgroundWith the re-popularity of metal-on-metal (MoM) bearing in recent years, the cobalt toxicity has been a cause for concern in the total hip replacement surgery by both physicians and patients.MethodsMG-63 cell line was cultured in vitro and incubated with cobalt (II) chloride (CoCl2) and/or with astaxanthin (ASX) for 24 h. MTT assay was conducted to evaluate the cell viability after cobalt exposure and ASX treatment. Fluorescence-activated cell sorting (FACS) analysis was performed to examine the reactive oxygen species (ROS) level. Quantitative real-time polymerase chain reaction (PCR) was adopted to determine the mRNA levels of related targets. And western blot analysis was used to examine the protein expressions. One-way ANOVA with posttest Newman-Keuls multiple comparisons was adopted to analysis all the obtained data.ResultsIn the current study, ASX exhibited significant protective effect against the Co(II)-induced cytotoxicity in MG-63 cell line. We also found that ASX protected the cells against Co-induced apoptosis by regulating the expression of Bcl-2 family proteins. Besides, heme oxygenase 1 (HO-1) could be activated by Co exposure; ASX treatment significantly inhibited HO-1 activation, suppressing the oxidative stress induced by Co exposure. Moreover, c-Jun N-terminal Kinase (JNK) phosphorylation was shown to participate in the signaling pathway of the protective effect of ASX. However, knockdown of JNK expression by siRNA transfection or JNK inhibitor SP600125 treatment did not affect the protective effect of ASX against cobalt cytotoxicity in MG-63 cells.ConclusionsASX mitigated cobalt cytotoxicity in the MG-63 cells by modulating the oxidative stress. And ASX could be a promising therapy against cobalt toxicity in the hip articulation surgery.Electronic supplementary materialThe online version of this article (doi:10.1186/s40360-017-0166-1) contains supplementary material, which is available to authorized users.

show abstract

“…Moreover, JNK acts as a negative regulator of p53 tumor suppressor to suppress p53-dependent senescence in mouse embryonic fibroblasts [91]. An increase of intracellular ROS levels can suppress the growth of cancer cells and induce cellular apoptosis by mediating MAPK signaling components [92].…”

Section: Signaling Pathways Involved In Cellular Senescencementioning

confidence: 99%

A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration

et al. 2019

View full text Add to dashboard Cite

Cellular senescence is a major hurdle for primary cell-based tissue engineering and regenerative medicine. Telomere erosion, oxidative stress, the expression of oncogenes and the loss of tumor suppressor genes all may account for the cellular senescence process with the involvement of various signaling pathways. To establish immortalized cell lines for research and clinical use, strategies have been applied including internal genomic or external matrix microenvironment modification. Considering the potential risks of malignant transformation and tumorigenesis of genetic manipulation, environmental modification methods, especially the decellularized cell-deposited extracellular matrix (dECM)-based preconditioning strategy, appear to be promising for tissue engineering-aimed cell immortalization. Due to few review articles focusing on this topic, this review provides a summary of cell senescence and immortalization and discusses advantages and limitations of tissue engineering and regeneration with the use of immortalized cells as well as a potential rejuvenation strategy through combination with the dECM approach.

show abstract

Calf Spleen Extractive Injection (CSEI), a small peptides enriched extraction, induces human hepatocellular carcinoma cell apoptosis via ROS/MAPKs dependent mitochondrial pathway

Cited by 11 publications

References 43 publications

Clinical Efficacy of Laparoscopic Billroth II Subtotal Gastrectomy Plus Lienal Polypeptide Injection for Gastric Cancer

Clinical Efficacy of Laparoscopic Billroth II Subtotal Gastrectomy Plus Lienal Polypeptide Injection for Gastric Cancer

Astaxanthin mitigates cobalt cytotoxicity in the MG-63 cells by modulating the oxidative stress

A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration

Contact Info

Product

Resources

About