Calcium Signaling and Its Significance in Alleviating Salt Stress in Plants

Srivastava, Ashish Kumar; N., A.; Patade, Vikas Yadav; Suprasanna, Penna

doi:10.1007/978-1-4614-6108-1_9

Cited by 20 publications

(13 citation statements)

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“…By annotation of targets, a few transcripts were found likely to participate in plant abiotic stress responses. For instance, a miR414 target was CBL-interacting serine/threonine-protein kinase 21 ( CIPK21 ), which was related to stress signal perception and transduction [ 32 ]; miR397a targeted laccases ( LACs ), which were involved in lignification and thickening of the cell wall [ 33 ]. In general, these results implied that these putative target genes might be implicated in diverse biological processes under salt stress in radish.…”

Section: Resultsmentioning

confidence: 99%

“…Apart from key TFs, a number of genes which encode important enzymes or functional proteins, such as APX1, CSD1, APSs (APS1 and APS4), LACs (LAC2, LAC11 and LAC17), UBCs (UBC17 and UBC24), Ca 2+ -mediated signal-related proteins (CAM7, CIPK21 and CDPK9), were also considered to play important roles in salt stress response. Among them, CIPK21 , CAM7 and CDPK9 (targeted by miR414, rsa-mir3 and rsa-mir5, respectively) were reported to cooperatively perform their functions to initiate the stimulus-specific downstream signal transduction [ 32 ]. miR397-targeted LACs encoding laccases were related to lignification and thickening of the cell wall in secondary cell growth [ 33 ], and accordingly enhanced the ability to alleviate stress damage.…”

Section: Discussionmentioning

confidence: 99%

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Identification of novel and salt-responsive miRNAs to explore miRNA-mediated regulatory network of salt stress response in radish (Raphanus sativus L.)

et al. 2015

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BackgroundSalt stress is one of the most representative abiotic stresses that severely affect plant growth and development. MicroRNAs (miRNAs) are well known for their significant involvement in plant responses to abiotic stresses. Although miRNAs implicated in salt stress response have been widely reported in numerous plant species, their regulatory roles in the adaptive response to salt stress in radish (Raphanus sativus L.), an important root vegetable crop worldwide, remain largely unknown.ResultsSolexa sequencing of two sRNA libraries from NaCl-free (CK) and NaCl-treated (Na200) radish roots were performed for systematical identification of salt-responsive miRNAs and their expression profiling in radish. Totally, 136 known miRNAs (representing 43 miRNA families) and 68 potential novel miRNAs (belonging to 51 miRNA families) were identified. Of these miRNAs, 49 known and 22 novel miRNAs were differentially expressed under salt stress. Target prediction and annotation indicated that these miRNAs exerted a role by regulating specific stress-responsive genes, such as squamosa promoter binding-like proteins (SPLs), auxin response factors (ARFs), nuclear transcription factor Y (NF-Y) and superoxide dismutase [Cu-Zn] (CSD1). Further functional analysis suggested that these target genes were mainly implicated in signal perception and transduction, regulation of ion homeostasis, basic metabolic processes, secondary stress responses, as well as modulation of attenuated plant growth and development under salt stress. Additionally, the expression patterns of ten miRNAs and five corresponding target genes were validated by reverse-transcription quantitative PCR (RT-qPCR).ConclusionsWith the sRNA sequencing, salt-responsive miRNAs and their target genes in radish were comprehensively identified. The results provide novel insight into complex miRNA-mediated regulatory network of salt stress response in radish, and facilitate further dissection of molecular mechanism underlying plant adaptive response to salt stress in root vegetable crops.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1416-5) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of novel and salt-responsive miRNAs to explore miRNA-mediated regulatory network of salt stress response in radish (Raphanus sativus L.)

et al. 2015

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“…In our proteomic analysis, several key calcium-signaling components e.g., calmodulin (CAMs), calmodulin-like proteins (CMLs) and calcium-dependent protein kinases (CDPKs) were identified with altered abundance (Table 2 ; Table S5 ). It is well established that CAMs, CMLs, and CDPKs act as important Ca 2+ signaling sensors in this signaling process (Srivastava et al, 2013 ). Furthermore, several other protein species related to calcium signaling pathway, including 14-3-3-like proteins, annexin, calreticulin, phospholipase C (PLC) and phospholipase D (PLD), were up-accumulated under salt stress (Table 2 ; Table S5 ).…”

Section: Discussionmentioning

confidence: 99%

Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.)

Sun

Wang

et al. 2017

Front. Plant Sci.

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To understand the molecular mechanism underlying salt stress response in radish, iTRAQ-based proteomic analysis was conducted to investigate the differences in protein species abundance under different salt treatments. In total, 851, 706, and 685 differential abundance protein species (DAPS) were identified between CK vs. Na100, CK vs. Na200, and Na100 vs. Na200, respectively. Functional annotation analysis revealed that salt stress elicited complex proteomic alterations in radish roots involved in carbohydrate and energy metabolism, protein metabolism, signal transduction, transcription regulation, stress and defense and transport. Additionally, the expression levels of nine genes encoding DAPS were further verified using RT-qPCR. The integrative analysis of transcriptomic and proteomic data in conjunction with miRNAs was further performed to strengthen the understanding of radish response to salinity. The genes responsible for signal transduction, ROS scavenging and transport activities as well as several key miRNAs including miR171, miR395, and miR398 played crucial roles in salt stress response in radish. Based on these findings, a schematic genetic regulatory network of salt stress response was proposed. This study provided valuable insights into the molecular mechanism underlying salt stress response in radish roots and would facilitate developing effective strategies toward genetically engineered salt-tolerant radish and other root vegetable crops.

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“…In plants, DAG is converted to phosphatidic acid (PA), which is an important signal molecule in plant abiotic and biotic stress (Testerink and Munnik, 2011), InsP3 may be further phosphorylated to form inositol hexaphosphate (InsP6), both InsP3 and InsP6 are thought to release Ca 2+ from intracellular storage (Valentová and Martinec, 2007;Yang et al, 2010). When plants are exposed to external stimuli, they increase the intracellular Ca 2+ concentration, which in turn activates a series of calcium-binding proteins, including Ca 2+ sensors/decoders, protein kinases and transcription factors (Srivastava et al, 2013). Melatonin can change the expression of genes involved in signal transduction.…”

Section: Melatonin As a Signaling Molecule Induces A Second Messenger In Response To Salt Stressmentioning

confidence: 99%

Melatonin Improves Cotton Salt Tolerance by Regulating ROS Scavenging System and Ca2 + Signal Transduction

et al. 2021

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As one of the cash crops, cotton is facing the threat of abiotic stress during its growth and development. It has been reported that melatonin is involved in plant defense against salt stress, but whether melatonin can improve cotton salt tolerance and its molecular mechanism remain unclear. We investigated the role of melatonin in cotton salt tolerance by silencing melatonin synthesis gene and exogenous melatonin application in upland cotton. In this study, applicating of melatonin can improve salt tolerance of cotton seedlings. The content of endogenous melatonin was different in cotton varieties with different salt tolerance. The inhibition of melatonin biosynthesis related genes and endogenous melatonin content in cotton resulted in the decrease of antioxidant enzyme activity, Ca2+ content and salt tolerance of cotton. To explore the protective mechanism of exogenous melatonin against salt stress by RNA-seq analysis. Melatonin played an important role in the resistance of cotton to salt stress, improved the salt tolerance of cotton by regulating antioxidant enzymes, transcription factors, plant hormones, signal molecules and Ca2+ signal transduction. This study proposed a regulatory network for melatonin to regulate cotton’s response to salt stress, which provided a theoretical basis for improving cotton’s salt tolerance.

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Calcium Signaling and Its Significance in Alleviating Salt Stress in Plants

Cited by 20 publications

References 79 publications

Identification of novel and salt-responsive miRNAs to explore miRNA-mediated regulatory network of salt stress response in radish (Raphanus sativus L.)

Identification of novel and salt-responsive miRNAs to explore miRNA-mediated regulatory network of salt stress response in radish (Raphanus sativus L.)

Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.)

Melatonin Improves Cotton Salt Tolerance by Regulating ROS Scavenging System and Ca2 + Signal Transduction

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