Calcium regulates stanniocalcin mRNA levels in primary cultured rainbow trout corpuscles of Stannius

Wagner, Graham F.; Jaworski, Ewa

doi:10.1016/0303-7207(94)90023-x

Cited by 39 publications

(23 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The stimulatory effect of 1α,25-dihydroxyvitamin D 3 is consistent with the established hypocalcemic action of stanniocalcin and may be mediated through the elevated levels of plasma Ca 2+ [3, 4, 5]. A similar Ca 2+ -dependent regulation of the stanniocalcin gene has been demonstrated in the fish cells derived from the corpuscles of Stannius [35]. It has been reported that 1α,25-dihydroxyvitamin D 3 binds to the specific nuclear receptor and can directly activate the genes encoding Ca 2+ -mobilizing factors or suppress the genes for hypocalcemic factors [34].…”

Section: Discussionsupporting

confidence: 49%

Distribution of Stanniocalcin 1 in Rat Kidney and Its Regulation by Vitamin D<sub>3</sub>

Ookata

Tojo

Onozato

et al. 2001

Nephron Exp Nephrol

View full text Add to dashboard Cite

Stanniocalcin is a glycoprotein hormone first described in fish as a hypocalcemic factor, and recently its mammalian counterpart has been identified. Localization of stanniocalcin 1 and its regulation of expression were determined in control and 1α,25-dihydroxyvitamin D₃-treated rats. Immunoreactivity for stanniocalcin 1 was detected in the loop of Henle, macula densa cells, distal convoluted tubule (DCT), and cortical collecting duct (CCD), and also faintly in the medullary collecting ducts. Pre-embedding electron-microscopic immunocytochemistry revealed stanniocalcin 1 in the apical membrane of cells of loop of Henle, DCT, and principal cells of CCD. The expression of stanniocalcin 1 was increased by elevated plasma calcium via 1α,25-dihydroxyvitamin D₃ treatment. In conclusion, stanniocalcin 1 was expressed in the apical membrane of distal nephron segments and enhanced by vitamin D₃.

show abstract

Section: Discussionsupporting

confidence: 49%

Distribution of Stanniocalcin 1 in Rat Kidney and Its Regulation by Vitamin D<sub>3</sub>

Ookata

Tojo

Onozato

et al. 2001

Nephron Exp Nephrol

View full text Add to dashboard Cite

show abstract

“…Extracellular calciuminvoked upregulation of STC mRNA and/or protein synthesis have been demonstrated in SUSM-1 (Chang et al 1995) and Paju cells respectively, although the calcium levels required (5·4 mM) were considerably above the physiological range. In fish, both STC secretion and mRNA levels are regulated by changes in extracellular calcium within the physiological range (0·7-1·9 mM); however the overall importance of changes in intracellular calcium has not been investigated (Wagner & Jaworski 1994). Similarly, the importance of intracellular calcium levels to STC gene activity in mammalian nervous tissue also needs to be clarified.…”

Section: Discussionmentioning

confidence: 99%

Effects of dibutyryl cAMP on stanniocalcin and stanniocalcin-related protein mRNA expression in neuroblastoma cells

Wong

Yeung

Mak³

et al. 2002

Journal of Endocrinology

View full text Add to dashboard Cite

Stanniocalcin is a polypeptide hormone that was first reported in fish as a regulator of mineral metabolism. Its recent identification in mammals has opened a new area of investigation in basic and clinical endocrinology. In the present study, regulation of the stanniocalcin (STC) and stanniocalcin related protein (STCrP) genes were investigated in mouse neuroblastoma cells (Neuro-2A) in relation to neuronal cell differentiation. Neuro-2A is an undifferentiated cell line that contains measurable levels of STCrP mRNA, but undetectable levels of STC mRNA. Treatment of the cells with either dbcAMP (1-4 mM) or 50 µM euxanthone (PW1) resulted in extensive differentiation and neurite outgrowth. However, only neurites of dbcAMP-treated cells developed varicosities, a phenotypic marker of axon formation. Furthermore, following differentiation induced by dbcAMP, there was an upregulation of STC and downregulation of STCrP mRNA levels. In the first 24 and 48 h of treatments, there was a maximum twofold induction and 1·5-fold reduction in STC and STCrP mRNAs respectively. Following 96 h of treatment, an additional 14-fold STC induction and 1·2-fold STCrP reduction were observed. The increase in STC mRNA levels was accompanied by a concomitant increase in axon-specific low molecular form microtubuleassociated protein (MAP-2c) mRNA and varicosities on the neurites, suggesting a possible role for STC in axonogenesis. There was no induction of STC mRNA levels when PW1 was added into the culture media, whereas ionomycin (1-10 µM) had no observable effects on cell differentiation or STC/STCrP mRNA. Immunocytochemical staining of dbcAMP-treated cells revealed abundant levels of immunoreactive STC, particularly in the varicosities, with only weak staining in control, untreated cells. Antisense oligodeoxynucleotides transfection studies indicated that the expression of STC was a cause of varicosity formation and a consequence of cell differentiation. Our findings lend further support to the notion that STC is involved in the process of neural differentiation.

show abstract

“…Moreover, intraperitoneal injection of CaCl 2 into the rainbow trout and European eel stimulated STC release from the CS (279), as did acclimation of the eel to seawater versus fresh water (278). It appears that the Ca 2ϩ stimulation of CS secretion of STC may be direct and/or via cholinergic innervation (82,210), but it also may involve direct stimulation of transcription (781). A calcium-sensitive receptor (CaR) has been sequenced from rainbow trout CS, and transcripts are also expressed in the gills (628).…”

Section: Stanniocalcinmentioning

confidence: 99%

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Evans¹,

Piermarini²,

Choe³

2005

Physiological Reviews

2,295

1,788

View full text Add to dashboard Cite

The fish gill is a multipurpose organ that, in addition to providing for aquatic gas exchange, plays dominant roles in osmotic and ionic regulation, acid-base regulation, and excretion of nitrogenous wastes. Thus, despite the fact that all fish groups have functional kidneys, the gill epithelium is the site of many processes that are mediated by renal epithelia in terrestrial vertebrates. Indeed, many of the pathways that mediate these processes in mammalian renal epithelial are expressed in the gill, and many of the extrinsic and intrinsic modulators of these processes are also found in fish endocrine tissues and the gill itself. The basic patterns of gill physiology were outlined over a half century ago, but modern immunological and molecular techniques are bringing new insights into this complicated system. Nevertheless, substantial questions about the evolution of these mechanisms and control remain.

show abstract

Calcium regulates stanniocalcin mRNA levels in primary cultured rainbow trout corpuscles of Stannius

Cited by 39 publications

References 31 publications

Distribution of Stanniocalcin 1 in Rat Kidney and Its Regulation by Vitamin D<sub>3</sub>

Distribution of Stanniocalcin 1 in Rat Kidney and Its Regulation by Vitamin D<sub>3</sub>

Effects of dibutyryl cAMP on stanniocalcin and stanniocalcin-related protein mRNA expression in neuroblastoma cells

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Contact Info

Product

Resources

About