“…ACP can be easily biodegraded by cells and its degradation rate can be controlled. In addition, ACP can restrain aseptic inflammation, meaning that ACP can be an ideal candidate as a functional delivery system for chemotherapy of osteosarcoma [4]. Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Calcium phosphate (CaP) based nanoparticles are considered to be ideal drug carriers for delivery of anticancer drugs because of their excellent biocompatibility, bioactivity, and pH responsiveness [4]. Different from liposomes and polymer micelles, CaP nanoparticles are able to encapsulate various drugs in their rigid matrix to give an almost entire prevention of premature drug release in physiological condition of plasma (pH = 7.4) until they dissolve to calcium and phosphate ions in acidic environments such as in lysosomes (pH = 4.0–5.0) [1].…”
Background: Calcium phosphate (CaP) based nanoparticles are considered to be ideal drug carriers for delivery of anticancer drugs because of their excellent biocompatibility and pH responsiveness. However, CaP nanoparticles have the problems of limited drug load capacity, initial burst release, and short-term release. Thus, we prepared the CaP nanocomposites containing anticancer drug such as caffeic acid (CA-NP), chlorogenic acid (CG-NP), or cisplatin (CP-NP) in the presence of alginate as a polymer template to control the release rate of drugs. Results: The drug-loaded CaP nanocomposites exhibited spherical shape with a size of under 100 nm and the size of nanocomposites was hardly affected by the addition of drug. UV-visible spectroscopic analysis confirmed the insertion of drug into the CaP nanocomposites. These nanocomposites showed an initial burst release of drug, followed by a prolonged release, in which the release profile of drugs was depended on the solution pH. In addition, the drug-loaded CaP nanocomposites revealed anticancer activity on human osteosarcoma in a manner dependent on concentration of drugs and time. Conclusions: The drug-loaded CaP nanocomposites can contribute to the development of a new generation of controlled drug release carriers for chemotherapy of cancers.
“…ACP can be easily biodegraded by cells and its degradation rate can be controlled. In addition, ACP can restrain aseptic inflammation, meaning that ACP can be an ideal candidate as a functional delivery system for chemotherapy of osteosarcoma [4]. Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Calcium phosphate (CaP) based nanoparticles are considered to be ideal drug carriers for delivery of anticancer drugs because of their excellent biocompatibility, bioactivity, and pH responsiveness [4]. Different from liposomes and polymer micelles, CaP nanoparticles are able to encapsulate various drugs in their rigid matrix to give an almost entire prevention of premature drug release in physiological condition of plasma (pH = 7.4) until they dissolve to calcium and phosphate ions in acidic environments such as in lysosomes (pH = 4.0–5.0) [1].…”
Background: Calcium phosphate (CaP) based nanoparticles are considered to be ideal drug carriers for delivery of anticancer drugs because of their excellent biocompatibility and pH responsiveness. However, CaP nanoparticles have the problems of limited drug load capacity, initial burst release, and short-term release. Thus, we prepared the CaP nanocomposites containing anticancer drug such as caffeic acid (CA-NP), chlorogenic acid (CG-NP), or cisplatin (CP-NP) in the presence of alginate as a polymer template to control the release rate of drugs. Results: The drug-loaded CaP nanocomposites exhibited spherical shape with a size of under 100 nm and the size of nanocomposites was hardly affected by the addition of drug. UV-visible spectroscopic analysis confirmed the insertion of drug into the CaP nanocomposites. These nanocomposites showed an initial burst release of drug, followed by a prolonged release, in which the release profile of drugs was depended on the solution pH. In addition, the drug-loaded CaP nanocomposites revealed anticancer activity on human osteosarcoma in a manner dependent on concentration of drugs and time. Conclusions: The drug-loaded CaP nanocomposites can contribute to the development of a new generation of controlled drug release carriers for chemotherapy of cancers.
“…Various biomarkers are expressed in different stages of differentiation during osteodifferentiation. In general, there are three stages in the osteodifferentiation of MSCs: proliferation, matrix maturation, and matrix mineralization . The first stage features accelerated cell growth and increased expression of ALP.…”
Section: Resultsmentioning
confidence: 99%
“…The second stage (the early differentiation stage) involves increased expression of the Col1a1 matrix, one of the major components in the extracellular matrix, which accounts for 90% of the protein mass in the organic part of bone. [19b] The third stage is characterized by high levels of the extracellular matrix proteins, OCN and OPN, followed by the deposition of calcium phosphate minerals . As shown in Figure C, hMSCs grown on NAC/MS showed significantly higher expression of Col1a1 than those grown on AS and AMS at days 7 and 14.…”
Bio-scaffolds designed to mimic endogenous niches have been used extensively in stem cell therapy and tissue regeneration. However, limited entry of nutrients and cells inside the scaffold can lead to poor cell survival and proliferation, and scaffold implantation can require an invasive surgical approach. Here, a noninvasive method using injectable 3D porous micro-scaffolds with a bio-engine is developed for cell transplantation and tissue regeneration. A liquid nitrogen flash-frozen and immediately smashed method is developed to prepare 3D porous nanocarriers-alginate composite micro-scaffolds (NAC/MS).
Compared to common scaffolds, human mesenchymal stem cells (hMSCs) grown in NAC/ MS show high survival and proliferation rates; this is due to the small size of the micro-scaffolds, which allows access to nutrients. Bio-factors are loaded into nanocarriers to produce a bio-engine that can initiate and promote stem cells differentiation in a bionic manner, behaving like an engine. Transplanted hMSCs show significantly enhanced expansion and mineralization in vivo. Furthermore, NAC/MS can promote in situ regeneration of bone in cranial defects in dogs.The biomimetic NAC/MS provide an injectable niche to cell survival, proliferation, and fate control during tissue regeneration or cell therapy, which are critical for developing organoid culture devices and cells local delivery vehicles.
“…In addition, TiO 2 ‐based nanoarrays can also serve as drug vehicles for various therapeutic agents to achieve surface modification for the purpose of bacterial resistance, osteogenesis facilitation or tumor suppression . Recently, implantable device loaded with chemotherapeutic drugs have been administered in the treatment of different solid tumors . However, the acute toxicities and drug resistance of chemotherapeutic agents have imposed various degrees of limitations on their applications .…”
The incomplete removal of bone tumors leads to increased local recurrence and poor prognosis. To prevent ostoperative tumor recurrence and simultaneously repair surgery‐caused bone defects, there is a need of great significance to develop implantable biomaterials possessing both cancer cell‐killing ability and excellent bioactivity. In this work, a functionalized titanium‐based implant is successfully fabricated by loading curcumin (CUR) onto cyclodextrin based polymer (pCD) modified titanium dioxide (TiO2) nanorod arrays. Herein, a polydopamine (pDA) assisted film is implemented as a first coating layer onto the surface of the TiO2 nanoarrays to guarantee the robust anchorage of the pCD. The pCD coating acts as a reservoir for CUR, allowing for efficient drug loading and sustained release of anticancer drugs. Studies show that the CUR‐modified surfaces (TiO2/pDA/pCD/CUR) can significantly promote apoptosis of osteosarcoma cells in vitro by inducing mitochondrial dysfunction caused by the ROS overproduction, and meanwhile, effectively inhibit the tumor growth in vivo. Moreover, such functionalized implants with surface density of loaded CUR at 22.48 µg cm−2 or lower support the attachment and proliferation of osteoblasts in vitro. These results successfully demonstrate that as‐prepared TiO2/pDA/pCD/CUR constructs have combined anticancer performance and good biocompatibility, which has great promise for the surgical therapy of bone tumors.
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