Injectable 3D Porous Micro‐Scaffolds with a Bio‐Engine for Cell Transplantation and Tissue Regeneration

Luo, Zhuojing; Pan, Jijia; Sun, Yuhua; Zhang, Siqi; Yang, Yongmei; Liu, Hao; Li, Yan; Xu, Xiaoxue; Sui, Yi; Wei, Shicheng

doi:10.1002/adfm.201804335

Cited by 38 publications

(29 citation statements)

References 41 publications

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“…Matrices with nanofibrous structures have been widely used in studies of tissue regeneration due to their cytocompatibility and high surface area to support cell interactions. ,, In addition, injectable biomaterials are attractive for clinical use because of reduced impact and damage to surrounding tissues when compared to surgically implanted materials, as well as shorter surgical times and faster recovery of the tissue at the injection site. − Recently, injectable silk nanofiber hydrogels were developed in our group and exhibited improved tissue repair capacity for bone when compared with silk scaffolds and hydrogels without nanofibrous structures. − The silk nanofibers were mainly composed of beta-sheet (crystalline) structures, resulting in an interesting balance of hydrophobic and hydrophilic properties and more hydrophobic than other silk fibroins in aqueous solutions . The nanofibers also had higher negative charge density, which facilitated the dispersion of the nanofibers in water .…”

Section: Introductionmentioning

confidence: 99%

Injectable Silk Nanofiber Hydrogels for Sustained Release of Small-Molecule Drugs and Vascularization

Ding¹,

Zhou

Zhou³

et al. 2019

ACS Biomater. Sci. Eng.

101

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Strategies to control neovascularization in damaged tissues remain a key issue in regenerative medicine. Unlike most reported desferrioxamine (DFO)-loaded systems where DFO demonstrates a burst release, here we attain zero-order release behavior above 40 days. This outcome was achieved by blending DFO with silk nanofibers with special hydrophilic–hydrophobic properties. The special silk nanofibers showed strong physical binding capacity with DFO, avoiding chemical cross-linking. Using these new biomaterials in vivo in a rat wound model suggested that the DFO-loaded silk nanofiber hydrogel systems stimulated angiogenesis by the sustained release of DFO, but also facilitated cell migration and tissue ingrowth. These features resulted in faster formation of a blood vessel network in the wounds, as well improved healing when compared to the free DFO system. The DFO-loaded systems are also suitable for the regeneration of other tissues, such as nerve and bone, suggesting universality in the biomedical field.

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Section: Introductionmentioning

confidence: 99%

Injectable Silk Nanofiber Hydrogels for Sustained Release of Small-Molecule Drugs and Vascularization

Ding¹,

Zhou

Zhou³

et al. 2019

ACS Biomater. Sci. Eng.

101

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“…2e1 ) as verified by Masson’s trichrome staining, and μHAp was distributed uniformly as confirmed by calcein staining using confocal laser scanning microscopy (CLSM) (Fig. 2e2 ) 6 , 31 , 32 . The high porosity (87 ± 9%) and pore size (359 ± 49 μm) similar to those for natural cancellous bone were observed by SEM (Fig.…”

Section: Resultsmentioning

confidence: 63%

“…Craniofacial bones are formed mainly through intramembranous ossification, in which the mesenchymal cells differentiate directly into osteoblasts, and subsequently develop into an organic/inorganic hybrid cross-linked cancellous bone structure 5 . The reemerging structure and composition of craniofacial bones might provide necessary environmental cues to create an appropriate niche for the migration, infiltration, proliferation, and differentiation of stem cells 6 – 8 . The formation of cranial bone coincides with capillary evolvement, which generates highly vascularized tissue for the timely supply of oxygen and nutrients to maintain skeletal development, integration, and homeostasis 9 , 10 .…”

Section: Introductionmentioning

confidence: 99%

An instantly fixable and self-adaptive scaffold for skull regeneration by autologous stem cell recruitment and angiogenesis

Liu

et al. 2022

Nat Commun

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Limited stem cells, poor stretchability and mismatched interface fusion have plagued the reconstruction of cranial defects by cell-free scaffolds. Here, we designed an instantly fixable and self-adaptive scaffold by dopamine-modified hyaluronic acid chelating Ca2+ of the microhydroxyapatite surface and bonding type I collagen to highly simulate the natural bony matrix. It presents a good mechanical match and interface integration by appropriate calcium chelation, and responds to external stress by flexible deformation. Meanwhile, the appropriate matrix microenvironment regulates macrophage M2 polarization and recruits endogenous stem cells. This scaffold promotes the proliferation and osteogenic differentiation of BMSCs in vitro, as well as significant ectopic mineralization and angiogenesis. Transcriptome analysis confirmed the upregulation of relevant genes and signalling pathways was associated with M2 macrophage activation, endogenous stem cell recruitment, angiogenesis and osteogenesis. Together, the scaffold realized 97 and 72% bone cover areas after 12 weeks in cranial defect models of rabbit (Φ = 9 mm) and beagle dog (Φ = 15 mm), respectively.

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“…In addition to the above two broad categories of fabrication techniques which are widely reported and applied in industrialization, some other original methods have also been developed such as 3D holographic lithography, [66,67] injection [68][69][70] and focused ion beam (FIB) technology. [25] Although these techniques are not mature enough in the fabrication of microelectrodes, they still provide feasible solutions to the construction issues about high loading, high precision, or 3D structures.…”

Section: Supplementary Methodsmentioning

confidence: 99%

Recent Advances in High‐Performance Microbatteries: Construction, Application, and Perspective

Zhu

Kan

et al. 2020

Small

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considered indispensable and stimulate great upsurge in interest on relevant researches. [1-4] Through integrating with multiple functional materials or devices, the miniaturized energy-storage device can power functional systems, such as microactuators, implantable biosensors, and wearable gadgets. [5,6] As far as the performance is concerned, the shift from disposable or nonrechargeable product to a high-energy output device or even complicated system has motivated the improvement of energy storage capability during the past decade. Generally, microsupercapacitor (MSC) possesses the advantages of fast charge/discharge rate and long cycling lifetime, making it possible for some maintenance-free wearable microelectronics and biomedical devices. [7,8] However, the energy storage gap about an order of magnitude still exists in comparison with microbattery (MB), and the insufficient energy supply restricts the widespread applications of MSC in the main power systems. [9] Serving as the primary choice for powering advanced miniaturized devices, MBs ensure sufficient energy density and maintain a stable voltage output. In general, a rechargeable battery consists of cathode, anode, and electrolyte in between, therefore it is the electrode that calls for miniaturization in size ranging from microns to centimeters. The total energy available in a MB generally depends on the active materials loaded on a certain small area. Based on this, some concepts of MB have been proposed, such as ultrathin microelectrode for high volumetric specific energy and somewhat thick microelectrode for high areal specific energy. [10,11] In terms of the dimensional ratio of MB, the thickness of microelectrode gives priority to the transport distance of ions and electrons, thus favoring the improvement in power density when compared with the macro one. [12,13] The decisive factor for the performance of MB is the reaction mechanism, and multiple battery forms have been expanded into miniaturized power supply as the supplement. Among the MBs mainly represented by lithium-ion batteries (LIBs) with organic electrolyte, various alkali metal ion batteries with abundant resources have been explored for possible substitution of lithium. [14-16] From the perspective of intrinsic safety, aqueous batteries have been developed with the merits of both inexpensive electrolyte and relatively higher power density. [17] Additionally, air batteries derived from fuel cells and ideally comprised of infinite O 2 supply for cathode reaction High-performance miniaturized energy storage devices have developed rapidly in recent years. Different from conventional energy storage devices, microbatteries assume the main responsibility for micropower supply, functionalization, and characterization platforms. Evolving from the essential goals for battery design of high power density, high energy density, and long lifetime, further practical demands for microbatteries (MBs) have been raised for the microfabrication technique and device design. Numerous studies have generally...

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Injectable 3D Porous Micro‐Scaffolds with a Bio‐Engine for Cell Transplantation and Tissue Regeneration

Cited by 38 publications

References 41 publications

Injectable Silk Nanofiber Hydrogels for Sustained Release of Small-Molecule Drugs and Vascularization

Injectable Silk Nanofiber Hydrogels for Sustained Release of Small-Molecule Drugs and Vascularization

An instantly fixable and self-adaptive scaffold for skull regeneration by autologous stem cell recruitment and angiogenesis

Recent Advances in High‐Performance Microbatteries: Construction, Application, and Perspective

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