“…The antibacterial activity was evaluated with the plate count method as previously described (He et al 2019). Crystal violet (Beyotime) and the LIVE/DEAD BacLight Viability Kit (Life Technologies Corporation) were used to determine the anti-biofilm effect according to the recommended protocol (He et al 2019; Mok et al 2021). Details are provided in the Appendix.…”
Dental caries is a dynamic disease induced by the unbalance between demineralization of dental hard tissues caused by biofilm and remineralization of them; however, although various effective remineralization methods have been well documented, it is a challenge to reestablish the balance by enhancing remineralization alone while ignoring the antibacterial therapy. Therefore, the integration of remineralizing and antibacterial technologies offers a promising strategy to halt natural caries progression in clinical practice. Here, the conception of interrupting dental caries (IDC) was proposed based on the development of dual-functional coating with remineralizing and antibacterial properties. In this study, bovine serum albumin (BSA) loaded octenidine (OCT) successfully to form a BSA-OCT composite. Subsequently, through fast amyloid-like aggregation, the phase-transited BSA-OCT (PTB-OCT) coating can be covered on teeth, resin composite, or sealant surfaces in 30 min by a simple smearing process. The PTB-OCT coating showed satisfactory effects in promoting the remineralization of demineralized enamel and dentin in vitro. Moreover, this coating also exerted significant acid-resistance stability and anti-biofilm properties. Equally importantly, this coating exhibited promising abilities in reducing the microleakage between the tooth and resin composite in vitro and preventing primary and secondary caries in vivo. In conclusion, this novel dual-functional PTB-OCT coating could reestablish the balance between demineralization and remineralization in the process of caries, thereby potentially preventing or arresting caries.
“…The antibacterial activity was evaluated with the plate count method as previously described (He et al 2019). Crystal violet (Beyotime) and the LIVE/DEAD BacLight Viability Kit (Life Technologies Corporation) were used to determine the anti-biofilm effect according to the recommended protocol (He et al 2019; Mok et al 2021). Details are provided in the Appendix.…”
Dental caries is a dynamic disease induced by the unbalance between demineralization of dental hard tissues caused by biofilm and remineralization of them; however, although various effective remineralization methods have been well documented, it is a challenge to reestablish the balance by enhancing remineralization alone while ignoring the antibacterial therapy. Therefore, the integration of remineralizing and antibacterial technologies offers a promising strategy to halt natural caries progression in clinical practice. Here, the conception of interrupting dental caries (IDC) was proposed based on the development of dual-functional coating with remineralizing and antibacterial properties. In this study, bovine serum albumin (BSA) loaded octenidine (OCT) successfully to form a BSA-OCT composite. Subsequently, through fast amyloid-like aggregation, the phase-transited BSA-OCT (PTB-OCT) coating can be covered on teeth, resin composite, or sealant surfaces in 30 min by a simple smearing process. The PTB-OCT coating showed satisfactory effects in promoting the remineralization of demineralized enamel and dentin in vitro. Moreover, this coating also exerted significant acid-resistance stability and anti-biofilm properties. Equally importantly, this coating exhibited promising abilities in reducing the microleakage between the tooth and resin composite in vitro and preventing primary and secondary caries in vivo. In conclusion, this novel dual-functional PTB-OCT coating could reestablish the balance between demineralization and remineralization in the process of caries, thereby potentially preventing or arresting caries.
“…The prepared sample was separated by utilizing a vacuum filtration apparatus and subsequently stored in a polyethylene bag maintaining an air seal. Eqn (1) shows the relevant reaction that occurred in the HAp synthesis.…”
Section: Methodsmentioning
confidence: 99%
“…There has been emerging interest in hydroxyapatite (HAp) due to its morphology, crystallinity, and chemical composition which are very similar to those of human hard tissue, 1 60–70% of which is composed of this biomaterial. 2 It is being widely used as a potential bone graft substitute material in orthopedic and dentistry fields, 3 and thus controlling its properties is very crucial for fruitful applications, notably 2.2 million people need bone grafting per year.…”
A well-known biomaterial, hydroxyapatite (HAp), was synthesized by employing a wet chemical precipitation method utilizing orthophosphoric acid and calcium hydroxide.
“…The acid-etching process changed the regular structure of the enamel surfaces and loosened the HA structure, 57 providing the enamel surfaces with countless tiny pores (Fig. S2, ESI †), which will be beneficial for the deposition of exogenous Ca and P. 58 As can be seen from Fig. 5A-D, the newly formed minerals of all groups treated with DIW, PEG-PAsp and PEG-PAsp-ALN increased with the immersing time going by, indicating that three groups of enamel samples had certain remineralization ability.…”
Section: Remineralization Capability Of Peg-pasp-alnmentioning
Dental caries is a biofilm-induced bacterial infectious oral disease , where the early attachment of proteins and pathogenic bacteria to tooth surfaces has been known as the main cause of...
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