Calcium phosphate composite layers for surface-mediated gene transfer

Oyane, Ayako; Wang, Xiupeng; Sogo, Yu; Ito, Atsuo; Tsurushima, Hideo

doi:10.1016/j.actbio.2012.02.003

Cited by 95 publications

(97 citation statements)

References 150 publications

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“…The DLp-Ap layers were formed on a polymer substrate by an ACP-assisted biomimetic coating process that has been used for formation of apatite-based composite layers [16][17][18][26][27][28]. In this coating process, a polymer substrate is precoated with amorphous calcium phosphate (ACP) [29] that is a precursor of apatite and then immersed in a coating solution.…”

Section: Introductionmentioning

confidence: 99%

“…In this coating process, a polymer substrate is precoated with amorphous calcium phosphate (ACP) [29] that is a precursor of apatite and then immersed in a coating solution. The coating solutions used in this study were supersaturated calcium phosphate solutions (CP solutions [16][17][18][26][27][28][29][30]) supplemented with DNA-Lp complexes. The kind of Lp and the concentration of DNALp complexes in the coating solution were optimized for efficiency of gene transfer to epithelial-like CHO-K1 cells on the resulting DLp-Ap layer in the presence of serum.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer

Oyane

Yazaki

Araki

et al. 2012

J Mater Sci: Mater Med

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A surface-mediated gene transfer system using biocompatible apatite-based composite layers has great potential for tissue engineering. Among the apatite-based composite layers developed to date, we focused on a DNA-lipid-apatite composite layer (DLp-Ap layer), which has the advantage of relatively high efficiency as a non-viral system. In this study, various lipid transfection reagents, including a newly developed reagent, polyamidoamine dendron-bearing lipid (PD), were employed to prepare the DLp-Ap layer, and the preparation condition was optimized in terms of efficiency of gene transfer to epithelial-like CHO-K1 cells in the presence of serum. The optimized DLp-Ap layer derived from PD had the highest gene transfer efficiency among all the apatite-based composite layers prepared in this study. In addition, the optimized DLp-Ap layer demonstrated higher gene transfer efficiency in the presence of serum than the conventional particle-mediated systems using commercially available lipid transfection reagents. It was also shown that the optimized DLp-Ap layer mediated the area-specific gene transfer on its surface, i.e., DNA was preferentially transferred to the cells adhering to the surface of the layer. The present gene transfer system using the PD-derived DLp-Ap layer, with the advantages of high efficiency in the presence of serum and area-specificity, would be useful in tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer

Oyane

Yazaki

Araki

et al. 2012

J Mater Sci: Mater Med

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“…The DA-Ap layers are thought to form in the coating solutions via the growth of an apatite layer on the EVOH surface and the simultaneous immobilization of DNA and antibody, on the basis of our previous report on the ACP-assisted biomimetic coating process [35]. During this coating process, a crucial step affecting the DNA and antibody contents of the DA-Ap layer could be the adsorption of DNA and antibody molecules through electrostatic interactions on apatite crystals or their precursors including an amorphous phase [36][37][38][39] coating solution resulted in the higher mean antibody content of the resulting DA-Ap layer ( figure 3(b)), probably by accelerating the antibody adsorption rate. Sample DA20 had a lower DNA content than samples DA5 and DA10 ( figure 3(a)), although the initial DNA concentration in the coating solutions was the same.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of DNA-antibody–apatite composite layers for cell-targeted gene transfer

Yazaki

Oyane

Araki

et al. 2012

Science and Technology of Advanced Materials

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Surface-mediated gene transfer systems using apatite (Ap)-based composite layers have received increased attention in tissue engineering applications owing to their safety, biocompatibility and relatively high efficiency. In this study, DNA-antibody-apatite composite layers (DA-Ap layers), in which DNA and antibody molecules are immobilized within a matrix of apatite nanocrystals, were fabricated using a biomimetic coating process. They were then assayed for their gene transfer capability for application in a specific cell-targeted gene transfer. A DA-Ap layer that was fabricated with an anti-CD49f antibody showed a higher gene transfer capability to the CD49f-positive CHO-K1 cells than a DNA-apatite composite layer (D-Ap layer). The antibody facilitated the gene transfer capability of the DA-Ap layer only to the specific cells that were expressing corresponding antigens. When the DA-Ap layer was fabricated with an anti-N-cadherin antibody, a higher gene transfer capability compared with the D-Ap layer was found in the N-cadherin-positive P19CL6 cells, but not in the N-cadherin-negative UV♀2 cells or in the P19CL6 cells that were pre-blocked with anti-N-cadherin. Therefore, the antigen-antibody binding that takes place at the cell-layer interface should be responsible for the higher gene transfer capability of the DA-Ap than D-Ap layer. These results suggest that the DA-Ap layer works as a mediator in a specific cell-targeted gene transfer system.

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“…However, the biomolecules are highly susceptive to physiological diffusion and degradation processes, leading to a short half-life [28,29]. Ap is a promising matrix for biomolecules to maintain their bioactivity and to induce persistent signalling [30][31][32][33][34][35][36][37]. Besides, Ap, as the main inorganic component of human hard tissues, has good biocompatibility and has been reported to show good thromboresistance [9].…”

Section: Introductionmentioning

confidence: 99%

Improvement in endothelial cell adhesion and retention under physiological shear stress using a laminin–apatite composite layer on titanium

Wang

Maruyama

et al. 2013

J. R. Soc. Interface.

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Apatite (Ap), laminin-apatite composite (L5Ap, L10Ap, L20Ap and L40Ap) and albumin -apatite (AlbAp) composite layers were prepared on titanium (Ti) using a supersaturated calcium phosphate solution supplemented with laminin (0, 5, 10, 20 and 40 mg ml 21 ) or albumin (800 mg ml 21 ). With an increase in the concentrations of laminin in the supersaturated calcium phosphate solutions, the amounts of laminin immobilized on the Ti increased. The number of human umbilical vein endothelial cells (HUVECs) adhered to the laminin-apatite composite layers were remarkably higher than those to the untreated Ti, Ap layer and AlbAp composite layer. The number of cells adhered to the L40Ap was 4.3 times the untreated Ti. Moreover, cells adhered to the laminin-apatite composite layers showed significantly higher cell retention under the physiological shear stress for 1 h and 2 h than those to the untreated Ti, Ap layer and AlbAp composite layer. The number of cells remaining on the L40Ap under the physiological shear stress for 2 h was 9.5 times that of the untreated Ti. The laminin -apatite composite layer is a promising interfacial layer for endothelialization of blood-contacting materials.

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Calcium phosphate composite layers for surface-mediated gene transfer

Cited by 95 publications

References 150 publications

Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer

Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer

Fabrication of DNA-antibody–apatite composite layers for cell-targeted gene transfer

Improvement in endothelial cell adhesion and retention under physiological shear stress using a laminin–apatite composite layer on titanium

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