Calcium oxalate precipitation by diffusion using laminar microfluidics: toward a biomimetic model of pathological microcalcifications

Laffite, Guillaume; Leroy, César; Bonhomme, Christian; Bonhomme-Coury, Laure; Letavernier, Emmanuel; Daudon, Michel; Frochot, Vincent; Haymann, Jean Philippe; Rouzière, Stéphan; Lucas, Ivan T.; Bazin, Dominique; Babonneau, Florence; Abou‐Hassan, Ali

doi:10.1039/c6lc00197a

Cited by 43 publications

(49 citation statements)

References 20 publications

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“…In Figure 6, it is possible to observe diffraction peaks that are attributable to both COD as COM crystals. However, more intense peaks, detected at 2θ, such as 15 • , 25 • , 30 • and 31 • , correspond to crystal planes {1 0 1}, {2 1 1}, {2 0 2} and {0 2 0} of COM crystals respectively, which confirm what is observed by the above analysis [43,[71][72][73][74]. In general, the use of this kind of functionalized DSPF film as a solid organic template could contribute to understanding the mechanisms underlying the formation of biologically important minerals, such as pathological urinary crystal forms, and it is kinetic on surface deposition.…”

Section: Resultssupporting

confidence: 78%

Effect of Diazotated Sulphonated Polystyrene Films on the Calcium Oxalate Crystallization

et al. 2017

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Pathological crystallization of calcium oxalate (CaOx) inside the urinary tract is called calculi or kidney stone (Urolithiasis). CaOx exhibits three crystalline types in nature: CaOx monohydrate COM, dihydrate COD and trihydrate COT. COD and COM are often found in urinary calculi, particularly COM. Langmuir monolayers, membrane vesicles, phospholipids' micelles, among others, have been adopted as simplified biomimetic template-models to study in vitro the urolithiasis through CaOx. The nucleation and crystal growth of COM on self-assembled lipid monolayers have revealed that the negatively charged phosphatidylserine interface is a strong promoter of COM. Herein, we describe the synthesis and physicochemical characterization of diazotated sulphonated polystyrene films (DSPFs), prepared from various aminocompounds varying their polarity degree i.e., polar, non-polar and acidic DSPF derivatives. We also used these DSPFs as polymeric templates in crystallization experiments of CaOx in vitro. Images obtained by optical microscopy and scanning electron microscopy confirmed the precipitation of COM crystals on the DSPF surface. The employment of functionalized polymeric films as templates for CaOx crystallization represents a viable approach for understanding inorganic mineralization.

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Section: Resultssupporting

confidence: 78%

Effect of Diazotated Sulphonated Polystyrene Films on the Calcium Oxalate Crystallization

et al. 2017

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“…In addition, irreversibly sealed devices are not suitable for the extraction of solids and their ex situ characterization. As an alternative, Laffite el al . and Zeng et al .…”

Section: Methodology Of Microfluidics With Laminar Co‐flowsmentioning

confidence: 99%

“…As an alternative, Laffite el al. [29] and Zeng et al [30] used microfluidic devices that are reversibly sealed with a compressive holder ( Figure 2b). This approach allowed the study of calcium oxalate and calcium carbonate systems.…”

Section: Device Constructionsmentioning

confidence: 99%

“…These conditions drive the self-organization of the precipitate membranes with possible spatial variations in important aspects such as crystal morphologies and chemical compositions. For example, Laffite et al [29] observed two forms of calcium oxalate minerals in a microfluidic channel (Figure 8a). On the side of the sodium oxalate solution, the majority of crystals were twinned whewellite, CaC 2 O 4 · H 2 O.…”

Section: Self-organized Compositional Gradientsmentioning

confidence: 99%

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Materials Synthesis and Catalysis in Microfluidic Devices: Prebiotic Chemistry in Mineral Membranes

Wang

Steinbock

2019

ChemCatChem

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The processes that led to the origins of life possibly occurred in the inorganic precipitate membranes of alkaline hydrothermal vents. These geochemical systems provide spatial confinement, cross‐membrane gradients, and catalytic surfaces. The experimental exploration of catalysis in these materials is challenging due to the vastness of the underlying parameter space and the need to maintain nonequilibrium conditions for long times. Microfluidic approaches offer an efficient solution to some of these problems by allowing the formation of mineral membranes at the interface of flowing reactant solutions and the control of steep gradients. In this minireview, we summarize recent progress in the production of mineral membranes using laminar microfluidic devices and discuss their catalytic properties in the context of prebiotic chemistry. Examples of catalytic reactions include the formation of pyrophosphate, peptides, and thioesters in precipitates containing iron, nickel, and calcium.

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“…96,97 In our case, we have used several experimental setups to characterise or mimic the pathogenesis process. 98,99 Among them, let us highlight transmission electron microscopy 97 and nuclear magnetic resonance 100 (Figure 4).…”

Section: Characterisation Of Pcsmentioning

confidence: 99%

Physicochemistry in medicine: some selected examples

Bazin

Daudon

2019

J. Spectral Imaging

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Research on pathological calcifications constitutes an exciting topic at the interface between physics, chemistry and medicine. The relationship between their physicochemical characteristics and the pathology responsible for their formation offers a unique opportunity to perform a significant medical diagnosis, to assess the interaction between drugs and these biological entities as well as to develop new drugs. Regarding synchrotron radiation, the emergence of microbeam allows the clinician to perform an early diagnosis. Indeed, we will start this review with a clinical case where Fourier transform infrared spectroscopy using synchrotron radiation as a probe allowed the clinician to save the kidney function of a patient. Following this example, we will see that investigations on pathological calcifications constitute an elegant way to gather major information on different public health problems such type 2 diabetes as well as on rare diseases. To attain this goal, this mini-review dedicated to structural and chemical investigations and based on selected and recent data collected through techniques using third generation synchrotron radiation as a probe is proposed to the reader.

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Calcium oxalate precipitation by diffusion using laminar microfluidics: toward a biomimetic model of pathological microcalcifications

Cited by 43 publications

References 20 publications

Effect of Diazotated Sulphonated Polystyrene Films on the Calcium Oxalate Crystallization

Effect of Diazotated Sulphonated Polystyrene Films on the Calcium Oxalate Crystallization

Materials Synthesis and Catalysis in Microfluidic Devices: Prebiotic Chemistry in Mineral Membranes

Physicochemistry in medicine: some selected examples

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