Calcium oscillations, G1 phase duration and neurogenesis timing

Lenos, M.; Tsaniklidou, Sofia-Maria

doi:10.1016/j.tcb.2010.07.004

Cited by 4 publications

(3 citation statements)

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“…Further, p21 accumulation induced by calpain inhibition has also been observed in other studies, where in vitro incubation with calpain 1 and 2 resulted in rapid degradation of p21 [54],[55]. It has been demonstrated that calcium oscillations occurring in G1 to S transition are required for cell cycle progression in both neural progenitor and undifferentiated cells, correlating with G1 shortening and increased proliferation [22], [56]. Furthermore, calcium oscillations increase the levels of several proliferation-associated proteins and decrease p27-mediated inhibition [22].…”

Section: Discussionsupporting

confidence: 59%

“…Furthermore, calcium oscillations increase the levels of several proliferation-associated proteins and decrease p27-mediated inhibition [22]. Curiously, it was recently proposed that the length of G1 directly influences the differentiation rate of neural precursors [39], [56], [57]. In this respect, it appears that G1 phase prolongation is both necessary and sufficient to induce switching from proliferation to differentiation in neural progenitors [39].…”

Section: Discussionmentioning

confidence: 99%

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Distinct Regulatory Functions of Calpain 1 and 2 during Neural Stem Cell Self-Renewal and Differentiation

et al. 2012

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Calpains are calcium regulated cysteine proteases that have been described in a wide range of cellular processes, including apoptosis, migration and cell cycle regulation. In addition, calpains have been implicated in differentiation, but their impact on neural differentiation requires further investigation. Here, we addressed the role of calpain 1 and calpain 2 in neural stem cell (NSC) self-renewal and differentiation. We found that calpain inhibition using either the chemical inhibitor calpeptin or the endogenous calpain inhibitor calpastatin favored differentiation of NSCs. This effect was associated with significant changes in cell cycle-related proteins and may be regulated by calcium. Interestingly, calpain 1 and calpain 2 were found to play distinct roles in NSC fate decision. Calpain 1 expression levels were higher in self-renewing NSC and decreased with differentiation, while calpain 2 increased throughout differentiation. In addition, calpain 1 silencing resulted in increased levels of both neuronal and glial markers, β-III Tubulin and glial fibrillary acidic protein (GFAP). Calpain 2 silencing elicited decreased levels of GFAP. These results support a role for calpain 1 in repressing differentiation, thus maintaining a proliferative NSC pool, and suggest that calpain 2 is involved in glial differentiation.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Distinct Regulatory Functions of Calpain 1 and 2 during Neural Stem Cell Self-Renewal and Differentiation

et al. 2012

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“…In CNS development, calcium was proposed as one of the internal cues that control neurogenic timing linked with the cell cycle (Lenos and Tsaniklidou, 2011). Moreover, calcium oscillations have been reported in P19 cells, neurospheres, and the ventricular zone (VZ) of embryonic mice (Scemes et al , 2003; Weissman et al , 2004; Resende et al , 2010).…”

Section: Discussionmentioning

confidence: 99%