‘Calcium is life’

Feijó, José A.; Wudick, Michael M.

doi:10.1093/jxb/ery279

Cited by 20 publications

(10 citation statements)

References 30 publications

(41 reference statements)

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“…Though a large volume of work has answered a considerable number of questions regarding the mechanisms of calcium signaling in plants, many remain. These include where the ion is stored, the identity of the channels responsible for calcium entry into cells, the gating and regulation of said channels, and perhaps most significantly, how cells differentially interpret different calcium signals like waves, gradients, and oscillations [14]. It has been widely assumed that calcium enters pollen tubes through one or more classes of ion channel and several have been implicated.…”

Section: Calciummentioning

confidence: 99%

Signaling in Pollen Tube Growth: Beyond the Tip of the Polarity Iceberg

Scheible

McCubbin

2019

Plants

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The coordinated growth of pollen tubes through floral tissues to deliver the sperm cells to the egg and facilitate fertilization is a highly regulated process critical to the Angiosperm life cycle. Studies suggest that the concerted action of a variety of signaling pathways underlies the rapid polarized tip growth exhibited by pollen tubes. Ca2+ and small GTPase-mediated pathways have emerged as major players in the regulation of pollen tube growth. Evidence suggests that these two signaling pathways not only integrate with one another but also with a variety of other important signaling events. As we continue to elucidate the mechanisms involved in pollen tube growth, there is a growing importance in taking a holistic approach to studying these pathways in order to truly understand how tip growth in pollen tubes is orchestrated and maintained. This review considers our current state of knowledge of Ca2+-mediated and GTPase signaling pathways in pollen tubes, how they may intersect with one another, and other signaling pathways involved. There will be a particular focus on recent reports that have extended our understanding in these areas.

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Section: Calciummentioning

confidence: 99%

Signaling in Pollen Tube Growth: Beyond the Tip of the Polarity Iceberg

Scheible

McCubbin

2019

Plants

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“…To date convincing evidences for the involvement of H 2 O 2 in controlling polar growth has been obtained and acknowledged (Maksimov et al , 2018, but the role for other ROS was unclear as they were usually considered all together as "total ROS" (Bell et al 2009;Mangano et al 2016;Feijó and Wudick 2018). We used fast growing lily pollen tubes as a model system to check the possible involvement of radical ROS in the regulation of polar growth.…”

Section: Discussionmentioning

confidence: 99%

Oxygen radicals and cytoplasm zoning in growing lily pollen tubes

Podolyan

Luneva

Breygina

2020

Preprint

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Recently redox-regulation of tip growth has been extensively studied, but differential sensitivity of growing cells to particular ROS and their subcellular localization is still unclear.Here we used specific dyes to provide mapping of H 2 O 2 and O • 2¯ in short and long pollen tubes. We found apical accumulation of H 2 O 2 and H 2 O 2 -producing organelles in the shank that were not colocalized with O • 2¯-producing mitochondria. Differential modulation of ROS content of the germination medium affected both growth speed and pollen tube morphology. Oxygen radicals affected ionic zoning: membrane potential and pH gradients. OH• caused depolarization all along the tube while O • 2¯ provoked hyperpolarization and cytoplasm alkalinization. O • 2¯ accelerated growth and reduced tube diameter, indicating that this ROS can be considered as pollen tube growth stimulator along with H 2 O 2 . Serious structural disturbances were observed upon exposure to OH• and H 2 O 2 and O • 2¯ quencher MnTMPP: pollen tube growth slowed down and ballooned tips formed in both cases, but in the presence of OH• membrane transport and organelle distribution was affected as well. OH•, thus, can be considered as a negative influence on pollen tubes which, presumably, have mechanisms for leveling it. The assumption was confirmed by EPR spectroscopy: pollen tubes actively reduce OH• content in the incubation medium.

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“…However, the accumulated evidence suggests also low-molecular communication between sporophyte tissues and pollen, in particular, through ion exchange and production of ROS [ 6 , 7 ]. The ability of pollen grains to respond to changes in ionic environment was discovered around 45 years ago [ 9 ], and recently, it became clear that ROS and ionic currents form a single regulatory system, since ROS production and the activity of ion transporters are tightly interrelated [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

ROS and Ions in Cell Signaling during Sexual Plant Reproduction

Breygina

Klimenko

2020

IJMS

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Pollen grain is a unique haploid organism characterized by two key physiological processes: activation of metabolism upon exiting dormancy and polar tube growth. In gymnosperms and flowering plants, these processes occur in different time frames and exhibit important features; identification of similarities and differences is still in the active phase. In angiosperms, the growth of male gametophyte is directed and controlled by its microenvironment, while in gymnosperms it is relatively autonomous. Recent reviews have detailed aspects of interaction between angiosperm female tissues and pollen such as interactions between peptides and their receptors; however, accumulated evidence suggests low-molecular communication, in particular, through ion exchange and ROS production, equally important for polar growth as well as for pollen germination. Recently, it became clear that ROS and ionic currents form a single regulatory module, since ROS production and the activity of ion transport systems are closely interrelated and form a feedback loop.

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‘Calcium is life’

Cited by 20 publications

References 30 publications

Signaling in Pollen Tube Growth: Beyond the Tip of the Polarity Iceberg

Signaling in Pollen Tube Growth: Beyond the Tip of the Polarity Iceberg

Oxygen radicals and cytoplasm zoning in growing lily pollen tubes

ROS and Ions in Cell Signaling during Sexual Plant Reproduction

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