Oxygen radicals and cytoplasm zoning in growing lily pollen tubes

Abstract

Recently redox-regulation of tip growth has been extensively studied, but differential sensitivity of growing cells to particular ROS and their subcellular localization is still unclear.Here we used specific dyes to provide mapping of H 2 O 2 and O • 2¯ in short and long pollen tubes. We found apical accumulation of H 2 O 2 and H 2 O 2 -producing organelles in the shank that were not colocalized with O • 2¯-producing mitochondria. Differential modulation of ROS content of the germination medium affected both growth speed and pollen tube morphology. Oxygen radicals affected ionic zoning: membrane potential and pH gradients. OH• caused depolarization all along the tube while O • 2¯ provoked hyperpolarization and cytoplasm alkalinization. O • 2¯ accelerated growth and reduced tube diameter, indicating that this ROS can be considered as pollen tube growth stimulator along with H 2 O 2 . Serious structural disturbances were observed upon exposure to OH• and H 2 O 2 and O • 2¯ quencher MnTMPP: pollen tube growth slowed down and ballooned tips formed in both cases, but in the presence of OH• membrane transport and organelle distribution was affected as well. OH•, thus, can be considered as a negative influence on pollen tubes which, presumably, have mechanisms for leveling it. The assumption was confirmed by EPR spectroscopy: pollen tubes actively reduce OH• content in the incubation medium.

OsMTD2‐mediated reactive oxygen species (ROS) balance is essential for intact pollen‐tube elongation in rice

“…In addition, pollen‐tube rupture and sperm release were mediated by high levels of ROS at the entrance to the female gametophyte (Duan et al ., 2014). Different ROS can be considered to exhibit accelerating or decelerating influences on pollen‐tube growth, which possibly act in vivo as positive or negative signals controlling pollen physiology (Podolyan et al ., 2020). We observed that ROS became active in the endomembrane of rice pollen grain once it hydrates and is apically distributed in growing pollen tubes.…”