“…The strongest argument that GOLAC is a Golgi channel is that no other channel with the properties of GOLAC has previously been described by others in recordings from endoplasmic reticulum (Simon and Blobel, 1991;Morier and Sauve, 1994;Duncan et al, 1997;Eliassi et al, 1997;Schmid et al, 1988), sarcoplasmic reticulum (Townsend and Rosenberg, 1995;Hals et al, 1989;Rousseau, 1989), or lysosomal fractions (Tilly et al, 1992). In addition, none of the single channels recorded from liver (the tissue source for our studies) were similar to GOLAC (plasma membranes (Henderson et al, 1989;Sellinger et al, 1992), organelles (mitochondria; Bowen et al, 1985;Costa et al, 1991;Selwyn et al, 1990), and peroxisomes (Labarca et al, 1986;Lemmens et al, 1989)). Second, the enrichment for Golgi markers in the cycloheximide Golgi fraction is orders of FIGURE 7 Conductance increased when lumenal pH was reduced.…”