Calcium Induced Release of Calcium from the Sarcoplasmic Reticulum of Skinned Skeletal Muscle Fibres

Endo, Makoto; Tanaka, Minoru; Ogawa, Yasuo

doi:10.1038/228034a0

Cited by 789 publications

(405 citation statements)

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“…La homeostasis del Ca 2+ celular y su señalización son el resultado de interacciones dinámicas entre mecanismos que conllevan a un incremento en la concentración intracelular de Ca 2+ y mecanismos que tienden a disminuirla, mediados por una diversidad de bombas y transportadores que se localizan en la membrana plasmática y en organelos intracelulares, como el retículo sarcoplásmico y la mitocondria, y por proteínas citosólicas que unen Ca 2+ . En el caso particular de los músculos estriados, los mecanismos de contracción y relajación están regulados por cambios en la concentración del Ca 2+ libre citoplásmico (o mioplásmico) (5)(6)(7)(8)(9). El fenómeno de acoplamiento excitación-contracción representa una comunicación rápida entre los eventos eléctricos que ocurren en la membrana plasmática o plasmalema y la liberación de Ca 2+ del retículo sarcoplásmico, la cual lleva a la contracción muscular (transducción de una señal eléctrica en una respuesta mecánica).…”

Section: El Mecanismo De Acoplamiento Excitación-contracción En El Múunclassified

“…La activación de los receptores de rianodina 1 genera la salida de Ca 2+ al citoplasma. Se ha propuesto que el Ca 2+ en la periferia del retículo sarcoplásmico activa otros receptores de rianodina no acoplados directamente con una tétrada de receptores de dihidropiridinas, mediante el mecanismo de retroalimentación positiva, inicialmente descrito en músculos de rana, conocido como liberación de Ca 2+ inducida por Ca 2+ (calcium-induced calcium release, CICR) (8,30); esto genera una liberación masiva de Ca 2+ que produce un incremento transitorio de este catión en el citoplasma de las fibras musculares. La presencia momentánea del Ca 2+ en el citoplasma se puede documentar en función del tiempo; el registro que se genera se conoce como transitorio de Ca 2+ (ver más adelante).…”

Section: El Mecanismo De Acoplamiento Excitación-contracción En El Múunclassified

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El acoplamiento excitación-contracción en el músculo esquelético: preguntas por responder a pesar de 50 años de estudio

Calderón-Vélez

Figueroa

2009

biomedica

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El mecanismo de acoplamiento excitación-contracción fue definido en el músculo esquelético como la secuencia de eventos que ocurre desde la generación del potencial de acción en la fibra muscular hasta que se inicia la generación de tensión. La regulación e interacción de dichos eventos entre sí ha sido estudiada durante los últimos 50 años utilizando diferentes técnicas, con las cuales se estableció la importancia y origen del ion calcio como activador contráctil, se conocen las principales proteínas involucradas y se inició el estudio de la base ultraestructural y de la regulación farmacológica; además, hay evidencias de que el acoplamiento excitación-contracción se altera en diferentes situaciones como en el envejecimiento, en la fatiga muscular y en algunas enfermedades musculares. Sin embargo, aún hay varias preguntas por responder: ¿cómo es el desarrollo y envejecimiento del mecanismo de acoplamiento excitación-contracción?, ¿cuál es su papel en la fatiga muscular y en algunas enfermedades musculares?, ¿cuál es la naturaleza de la interacción entre diferentes proteínas involucradas en el acoplamiento excitación-contracción? La presente revisión describe el acoplamiento excitación-contracción en el músculo esquelético y las técnicas utilizadas para su estudio como introducción para discutir algunas de las preguntas que aún falta por responder al respecto.Palabras clave: músculo esquelético, contracción muscular, relajación muscular, calcio, canal liberador de calcio, canales de calcio tipo L, receptor de rianodina, fatiga muscular. Excitation-contraction coupling in skeletal muscle: questions remaining after 50 years of researchThe excitation-contraction coupling mechanism was defined as the entire sequence of reactions linking excitation of plasma membrane to activation of contraction in skeletal muscle. By using different techniques, their regulation and interactions have been studied during the last 50 years, defining until now the importance and origin of the calcium ion as a contractile activator and the main proteins involved in the whole mechanism. Furthermore, the study of the ultrastructural basis and pharmacological regulation of the excitation-contraction coupling phenomenon has begun. The excitation-contraction coupling is thought to be altered in situations as ageing, muscle fatigue and some muscle diseases. However, many questions remain to be answered. For example, (1) How excitation-contraction coupling develops and ages? (2) What role does it play in muscle fatigue and other diseases? (3) What is the nature of the interaction between the proteins believed to be involved? The present review describes excitation-contraction coupling in skeletal muscle and techniques used to better understand it as an introduction for discussing unanswered questions regarding excitation-contraction coupling.

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Section: El Mecanismo De Acoplamiento Excitación-contracción En El Múunclassified

El acoplamiento excitación-contracción en el músculo esquelético: preguntas por responder a pesar de 50 años de estudio

Calderón-Vélez

Figueroa

2009

biomedica

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“…4~ Since sarcolemma and transverse tubules are not functional in our skinned fibres these membranes cannot serve as sources for "regenerative" calcium. However, Endo, et al 31 and Thorens,et al 4~ have reported that calcium induced calcium release from the sarcoplasmic rcticulum can occur in skinned fibres devoid of functional sarcolemma and transverse tubules. They believe, in fact, that caffeine induced calcium release from the sarcoplasmie reticulum is secondary to acceleration of calcium induced calcium release.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the caffeine skinned fibre tension (csft) test with the caffeine-halothane contracture (chc) test in the diagnosis of malignant hyperthermia

Britt

Frodis

Scott

et al. 1982

Canad. Anaesth. Soc. J.

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We have compared and contrasted two diagnostic tests for Malignant Hyperthermia (MH) -the Caffeine-Halothane Contracture Test and the Caffeine Skinned Fibre Tension Test. Both tests show a strongly positive relationship both with the occurrence of MH reactions and with each other. The former test is more rapid and requires less skill. The latter test can be performed on much less muscle and permits storage of the muscle over prolonged periods of time. of muscle required was small. Moreover, fibres prepared from muscle stored for several months in the freezer were still viable. We felt, therefore, that a diagnostic test for MH based on this preparation might have several advantages over the CHC test of intact muscle fascicles. Much less muscle would be needed. Experimental errors could be rectified by another trip to the freezer. Considerable information might be learned about the site and nature of the MH defect,In 1977, therefore, during the course of routine biopsies in suspected MH patients, in addition to obtaining muscle for our usual diagnostic tests (CHC test, 2'3 halothane-ATP depletion test (H-ATP-D), 3 and routine microscopyZ), we also excised small strips of muscle secured to wooden sticks. These were chemically skinned by the method of Wood z2-2s and were then sent to him by private courier for examination. Wood found that in malignant hyperthermic susceptible (MHS) muscle a larger than normal percentage of fibres developed tensions at lower than normal caffeine concentrations. 25 Because of these promising preliminary findings we decided to examine in detail in our own laboratory the value of this technique as a sole diagnostic test, as a complement to the CHC test and as an aid in learning more about the nature of the MH defect. The test has now been named the caffeine skinned fibre tension (CSFr) test. 550

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“…The contradiction, however, can be resolved when the entry of Ca is assumed to be a trigger for the Ca-induced Ca-release mechanism first reported in the skeletal muscle by ENDO et al (1970) and also in the guinea-pig taenia coli (ENDO et al, 1977). This assumption may be supported by the present results that K-induced contracture was inhibited markedly by procaine, as in the case of ACh-induced contracture which was thought to be highly dependent on intracellular stored Ca.…”

Section: Discussionmentioning

confidence: 99%

“…Ca release from an intracellular store may be carried out by the mechanism of Ca-induced Ca release (ENDO et al, 1970), because Ca-induced contracture was inhibited by procaine (Fig. 5), which is known to inhibit the Ca-induced Ca release mechanism in skeletal muscle (THORENS and ENDO, 1975).…”

Section: Discussionmentioning

confidence: 99%

Contribution of intracellular stored calcium to contractile activation in contractures of stomach circular muscle of Bufo vulgaris formosus.

Shinohara¹,

Isao²

1984

Jpn.J.Physiol

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Contractile responses of stomach circular muscle of Bufo to high-K, to acetylcholine (ACh) in normal Ringer or in high-K solution, and to calcium in Ca-free high-K solution showed a phasic contraction which relaxed completely in 30-45 sec. K-induced contracture was abolished in Ca-free solution containing 1 mM ethylene glycol bis(j3-aminoethyl ether)-N,N'-tetraacetic acid (EGTA) within 20 sec, while ACh-induced contracture was not abolished and 10-25 % of control tension was kept up to 40 sec. This response increased to 40-50% when all extracellular Na was replaced with tris(hydroxymethyl)-aminomethane (Tris). K-induced contracture was inhibited completely by 1 mM La. ACh-induced contracture in the muscle depolarized by high-K solution was dependent on the depolarization time, 0-10, b0-70, and nearly 100% of control after 1, 3, and 10 min depolarization, respectively. These ACh-induced contractures were not inhibited by 1 mM EGTA or La. All contractures mentioned above were markedly inhibited by 5 mM procaine. These results suggest that activation of both contractures induced by high-K and ACh were, at least partly, dependent on the Ca at the intracellular Ca storage sites. Ca-induced contracture was dependent on depolarization time as was ACh-induced contracture, when the muscle was depolarized by Ca-free high-K solution without pre-treatment with Ca-free Ringer solution. These results suggest that activation of Ca-contracture is also dependent on intracellular stored Ca.

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Calcium Induced Release of Calcium from the Sarcoplasmic Reticulum of Skinned Skeletal Muscle Fibres

Cited by 789 publications

References 9 publications

El acoplamiento excitación-contracción en el músculo esquelético: preguntas por responder a pesar de 50 años de estudio

El acoplamiento excitación-contracción en el músculo esquelético: preguntas por responder a pesar de 50 años de estudio

Comparison of the caffeine skinned fibre tension (csft) test with the caffeine-halothane contracture (chc) test in the diagnosis of malignant hyperthermia

Contribution of intracellular stored calcium to contractile activation in contractures of stomach circular muscle of Bufo vulgaris formosus.

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