Calcium Hydroxide–induced Proliferation, Migration, Osteogenic Differentiation, and Mineralization via the Mitogen-activated Protein Kinase Pathway in Human Dental Pulp Stem Cells

Chen, Luoping; Zheng, Lisha; Jiang, Jingyi; Gui, Jinpeng; Zhang, Lingyu; Huang, Yan; Chen, Xiaofang; Ji, Jing; Fan, Yubo

doi:10.1016/j.joen.2016.04.025

Cited by 43 publications

(41 citation statements)

References 33 publications

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“…Over 50 cells/well were scored as colonies and passaged by 0.25% trypsin (Sigma‐Aldrich, MO, USA). Cultured DPSCs displayed the characteristics mentioned in previous studies (Chen et al, ). Passages 3 to 6 of DPSCs were used in the experiments.…”

Section: Methodsmentioning

confidence: 99%

Static magnetic field regulates proliferation, migration, differentiation and YAP/TAZ activation of human dental pulp stem cells

Zheng

Zhang

Chen

et al. 2018

J Tissue Eng Regen Med

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The dental pulp stem cells (DPSCs) are a population of mesenchymal stem cells, which have multilineage potential and high proliferation. DPSCs are regarded as a promising tool for tissue regeneration of dentine, dental pulp, bone, cartilage, and muscle. Recently, magnetic materials have become commonly applied in dental clinics. Static magnetic field has been reported to regulate the proliferation, migration, or differentiation of stem cells. However, whether static magnetic fields affect DPSCs is still unknown. In our study, we investigated the effect of static magnetic field on the proliferation, migration, and differentiation of DPSCs. The results indicated that static magnetic field rearranged the cytoskeleton of DPSCs. A static magnetic field of 1 mT increased DPSC proliferation, as well as the gene expression of several growth factors such as FGF-2, TGF-β, and VEGF. Moreover, the static magnetic field promoted the migration of DPSCs by regulating MMP-1 and MMP-2 gene expression. Static magnetic field of 1 mT also induced osteo/odontogenesis and mineralization in DPSCs. Otherwise, the static magnetic field recruited YAP/TAZ to the nucleus, inhibited the phosphorylation of YAP/TAZ, and upregulated the two YAP/TAZ-regulated genes, CTGF and ANKRD1. Cytoskeleton inhibitor, cytochalasin D, obviously inhibited the nuclear localization of YAP/TAZ. When YAP/TAZ were knocked-down, the static magnetic field-induced mineralization of DPSCs was diminished. Our findings provide an insight into the effect of static magnetic field on DPSCs and provide the foundation for the future tissue regeneration.

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Section: Methodsmentioning

confidence: 99%

Static magnetic field regulates proliferation, migration, differentiation and YAP/TAZ activation of human dental pulp stem cells

Zheng

Zhang

Chen

et al. 2018

J Tissue Eng Regen Med

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“…Direct pulp capping strategies previously included the use of calcium hydroxide (Chen et al . ), recombinant growth factors or bioactive molecules (Iohara et al . , Zheng et al .…”

Section: Introductionmentioning

confidence: 99%

“…It involves directly sealing the pulpal wound surface with a protective dental material to facilitate the formation of tertiary dentine (Hilton et al 2013). Direct pulp capping strategies previously included the use of calcium hydroxide (Chen et al 2016), recombinant growth factors or bioactive molecules (Iohara et al 2004, Zheng et al 2009), transplantation of dental pulp stem cells via biocompatible carriers (Cvikl et al 2017), mineral trioxide aggregate (MTA) and bioceramics (Parirokh et al 2018). MTA has been widely accepted as the gold standard for Correspondence: Motoki Okamoto, Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan (tel.…”

Section: Introductionmentioning

confidence: 99%

Effect of tissue inhibitor of metalloprotease 1 on human pulp cells in vitro and rat pulp tissue in vivo

et al. 2019

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Aim To evaluate the dentinogenetic effects of tissue inhibitor of metalloprotease (TIMP1) on human pulp cells in vitro and rat pulp tissue in vivo. Methodology The effect of TIMP1 on pulp cell functions related to hard tissue formation as part of the wound healing process (i.e. biocompatibility, proliferation, differentiation and mineralized nodule formation) was evaluated in vitro and using a direct pulp capping experimental animal model in vivo. The effects of different‐sized cavity preparations on hard tissue formation induced by ProRoot MTA at 2 weeks were evaluated using micro‐computed tomography (micro‐CT). Tertiary dentine formation quality and quantity after pulp capping using TIMP1, ProRoot MTA and phosphate‐buffered saline (PBS) was also evaluated after 4 weeks using micro‐CT in term of dentine volume (DV), dentine mineral density (DVD) and histological analysis. The data were evaluated by Student's t‐test, one‐way ANOVA with Tukey's post hoc test, the Kruskal–Wallis test or the Steel–Dwass test. P values < 0.05 were considered statistically significant. Results TIMP1 significantly stimulated dental pulp stem cell proliferation, differentiation, and mineralization and was more biocompatible compared with the PBS control (P < 0.05). In the pulp capping model, the amount of tertiary dentine that formed was directly proportional to the size of the pulp exposure; greater amounts of tertiary dentine were observed in pulps with larger exposures after 2 weeks. 4‐week samples of TIMP1 and ProRoot MTA had similar characteristics, but both sample significantly induced tertiary dentine formation beneath the cavity compared with PBS (P < 0.05) under standardized cavity preparations. Conclusions TIMP1 has an important role in pulpal wound healing, which makes it a potential biological pulp capping material and candidate molecule for regenerative endodontic therapy.

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“…Due to the easily accessible source of dental tissues, dental-derived stem cells are considered suitable candidates for tissue engineering applications [1, 2]. Dental pulp stem cells (DPSCs), isolated from human dental pulp tissue, possess multilineage differentiation potential, including osteogenic, dentinogenic, adipogenic, chondrogenic, myogenic, and neurogenic differentiation [3]. Reflecting its special anatomical structure, dental pulp is often exposed to hypoxic conditions [4].…”

Section: Introductionmentioning

confidence: 99%

Deferoxamine-Induced Migration and Odontoblast Differentiation via ROS-Dependent Autophagy in Dental Pulp Stem Cells

Wang

Jiang

et al. 2017

Cell Physiol Biochem

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Background/Aims: As a vital degradation and recycling system, autophagy plays an essential role in regulating the differentiation of stem cells. We previously showed that iron chelator deferoxamine (DFO) could promote the repair ability of dental pulp stem cells (DPSCs). Here, we investigated the effect of DFO in autophagy and the role of autophagy in regulating the migration and odontoblast differentiation of DPSCs. Methods: Transmission electron microscopy, immunofluorescence staining and western blotting were performed to evaluate the autophagic activity of DPSCs. Transmigration assay, alkaline phosphatase staining/activity, alizarin red S staining and quantitative PCR were performed to examine the migration and odontoblast differentiation of DPSCs. Reactive oxygen species (ROS) levels and the effects of ROS scavenger in autophagy induction were also detected. Autophagy inhibitors (3-MA and bafilomycin A1) and lentiviral vectors carrying ATG5 shRNA sequences were used for autophagy inhibition. Results: Early exposure to DFO promoted the mineralization of DPSCs and increased autophagic activity. Autophagy inhibition suppressed DFO-induced DPSC migration and odontoblast differentiation. Furthermore, DFO treatment could induce autophagy partly through hypoxia-inducible factor 1α/B cell lymphoma 2/adenovirus E1B 19K-interacting protein 3 (HIF-1α/BNIP3) pathway in a ROS-dependent manner. Conclusion: DFO increased DPSC migration and differentiation, which might be modulated through ROS-induced autophagy.

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Calcium Hydroxide–induced Proliferation, Migration, Osteogenic Differentiation, and Mineralization via the Mitogen-activated Protein Kinase Pathway in Human Dental Pulp Stem Cells

Cited by 43 publications

References 33 publications

Static magnetic field regulates proliferation, migration, differentiation and YAP/TAZ activation of human dental pulp stem cells

Static magnetic field regulates proliferation, migration, differentiation and YAP/TAZ activation of human dental pulp stem cells

Effect of tissue inhibitor of metalloprotease 1 on human pulp cells in vitro and rat pulp tissue in vivo

Deferoxamine-Induced Migration and Odontoblast Differentiation via ROS-Dependent Autophagy in Dental Pulp Stem Cells

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