Calcium handling in <i>Sparus auratus</i>: effects of water and dietary calcium levels on mineral composition, cortisol and PTHrP levels

Abbink, W.; Bevelander, Gideon S.; Rotllant, Josep; Canário, Adelino V.M.; Flik, G.

doi:10.1242/jeb.01254

Cited by 31 publications

(26 citation statements)

References 41 publications

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“…Indeed, compared to untreated control fish in seawater, all experimental groups in these experiments show slightly elevated cortisol levels, although we discussed that these rises were very mild and considered still within the limits of values for non-stress situations (Abbink et al, 2004). We realise ourselves that even mild elevations of cortisol may affect neuroendocrine regulatory systems including the melatonin system (Larson et al, 2004).…”

Section: Introductionmentioning

confidence: 89%

“…At the time of the experiments (spring-summer), the fish weighed between 10 and 40 g body mass. The experimental setup and sampling procedures were described recently (Abbink et al, 2004;in press). In short, for the first series of experiments, four groups of fish were used.…”

Section: Fishmentioning

confidence: 99%

“…The water cal cium content was decreased by dilution of the seawater (34-2.5& salinity) and by doing so, the water calcium con centration decreased from 10 to 0.7 mmoll-1 (Abbink et al, 2004).…”

Section: Introductionmentioning

confidence: 98%

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Melatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.)

Abbink

Kulczykowska

Kalamarz

et al. 2008

General and Comparative Endocrinology

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Brain or blood plasma melatonin was analysed as a measure for pineal melatonin production in sea bream. Access to calcium was limited by diluting the seawater to 2.5& and removing calcium from the diet or by prolonged feeding of vitamin D-deficient diet. Inter actions/relations between melatonin and calcium balance and the hypercalcemic endocrines PTHrP and calcitriol were assessed. Restrict ing calcium availability in both water and diet had no effect on plasma melatonin, but when calcium was low in the water or absent from food, increased and decreased plasma melatonin was observed, respectively. Fish on a vitamin D-deficient diet (D-fish) showed decreased plasma calcitriol levels and remained normocalcemic. Decreased brain melatonin was found at all sampling times (10-22 weeks) in the D -fish compared to the controls. A positive correlation between plasma Ca2+ and plasma melatonin was found (R2 = 0.19; N = 41; P < 0.01) and brain melatonin was negatively correlated with plasma PTHrP (R2 = 0.78; N = 4; P < 0.05). The posi tive correlation between plasma levels of melatonin and Ca2+ provides evidence that melatonin synthesis is influenced by plasma Ca2+. The decreased melatonin production in the D -fish points to direct or indirect involvement of calcitriol in melatonin synthesis by the pineal organ in teleosts. The hypercalcemic factors PTHrP and calcitriol appeared to be negatively correlated with melatonin and this substantiates an involvement of melatonin in modulating the endocrine response to cope with hypocalcemia. It further points to the importance of Ca2+ in melatonin physiology.

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Section: Introductionmentioning

confidence: 89%

Section: Fishmentioning

confidence: 99%

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Melatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.)

Abbink

Kulczykowska

Kalamarz

et al. 2008

General and Comparative Endocrinology

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“…PTHrP was quantified in plasma by radioimmunoassay using heterologous antisera raised against human peptide (Danks et al, 1993;Devlin et al, 1996) and later with homologous antisera (Rotllant et al, 2003;Abbink et al, 2004Abbink et al, , 2006. As holds for elasmobranch species, PTHrP levels in teleostean plasma are consistently and significantly higher than these values for humans (basal circulating lev els of around 0.5-2.5 pmol l_1 in humans and 0.1 0.6 nmol l_1 in fish).…”

Section: Pthrp In Teleostsmentioning

confidence: 99%

“…We assessed the PTHrP system in juvenile sea bream confronted with calcium constraint in water and diet (Abbink et al, 2004. In gill tissue, expression levels of the pthrp and pthlr genes were up-reg ulated on the longer term after calcium constraint; short term (4 h) constraint did not provoke changes.…”

Section: Calcium Balancementioning

confidence: 99%

Parathyroid hormone-related protein in teleost fish

Abbink

Flik

2007

General and Comparative Endocrinology

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A brief description is given of the discovery of PTHrP and the roles of the peptide in mammalian physiology. Next, the occurrence of PTHrP in the earliest vertebrates, sharks, skates and fishes, is reviewed and the calciotropic functions of PTHrP are addressed more spe cifically in fishes. Parathyroid hormone-related protein (PTHrP) is a hypercalcemic hormone in teleostean fishes, but also has para-and autocrine functions. After the isolation and identification of fish PTHrP and PTHrP receptors and the subsequent development of recom binant protein and a real-time quantitative PCR, a calciotropic role of PTHrP in fish physiology could be assessed. PTHrP influences calcium physiology via regulation of calcium mobilisation from internal sources (bone and scales) and via calcium uptake from the envi ronment (water and diet). Continuous variations in the need for calcium and in the availability of environmental calcium require fast calciotropes to guarantee calcium balance, in which PTHrP is pivotal for the fish. PTHrP is essential in fish bone physiology, e.g. in min eralisation and calcium reabsorption from the scales. Moreover, PTHrP plays a role in vitellogenesis, cortisol production, regulation of renal Mrp2 activity and melatonin synthesis. The plethora of functions of PTHrP in fish concern endocrine, paracrine and autocrine (and possibly intracrine) functions; calciotropic actions of PTHrP at the organismal and cellular level are prominent in fish. The strong con servation of the pthrp gene in the vertebrate lineage and the N-terminal similarity of the coded proteins relates to the important role of PTHrP in calcium physiology that is of paramount importance to all physiological processes. Recent and ongoing studies will contribute to our rapidly expanding knowledge of the original physiological functions of PTHrP in teleost fish.

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