Calcium from LactoCalciumTMMilk Mineral after Digestion with Pepsin Stimulates Mineralized Bone Nodule Formation in Human Osteoblast-Like SaOS-2 Cellsin Vitroand May Be Rendered Bioavailablein Vivo

Rao, L.G.; Khan, Talat; Gluck, Gilbert

doi:10.1271/bbb.60219

Cited by 7 publications

(3 citation statements)

References 41 publications

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“…Rao et al (2007) used an in vitro solubility assay to measure calcium bioaccessibility from a commercial calcium-milk protein supplement. The results showed that the calcium present in this supplement was readily released by enzymatic digestion: with increasing pepsin concentration, more mineral was released from the supplement.…”

Section: Applications Of In Vitro Methods and Recommendationsmentioning

confidence: 99%

Application of in vitro bioaccessibility and bioavailability methods for calcium, carotenoids, folate, iron, magnesium, polyphenols, zinc, and vitamins B6, B12, D, and E

Etcheverry¹,

Grusak²,

Fleige³

2012

Front. Physio.

299

248

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A review of in vitro bioaccessibility and bioavailability methods for polyphenols and selected nutrients is presented. The review focuses on in vitro solubility, dialyzability, the dynamic gastrointestinal model (TIM)™, and Caco-2 cell models, the latter primarily for uptake and transport, and a discussion of how these methods have been applied to generate data for a range of nutrients, carotenoids, and polyphenols. Recommendations are given regarding which methods are most justified for answering bioaccessibility or bioavailability related questions for specific nutrients. The need for more validation studies in which in vivo results are compared to in vitro results is also discussed.

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Section: Applications Of In Vitro Methods and Recommendationsmentioning

confidence: 99%

Application of in vitro bioaccessibility and bioavailability methods for calcium, carotenoids, folate, iron, magnesium, polyphenols, zinc, and vitamins B6, B12, D, and E

Etcheverry¹,

Grusak²,

Fleige³

2012

Front. Physio.

299

248

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“…Numerous prior investigations scrutinized the bioavailability of calcium using in vitro cell models [20][21][22][23]. Concerns regarding the beneficial impacts of probiotics and isoflavones on calcium bioavailability have emerged as a rapidly expanding area of study.…”

Section: Introductionmentioning

confidence: 99%

Effects of Daidzein, Tempeh, and a Probiotic Digested in an Artificial Gastrointestinal Tract on Calcium Deposition in Human Osteoblast-like Saos-2 Cells

Harahap,

Olejnik,

Kowalska

et al. 2024

IJMS

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Adequate calcium intake is crucial for the prevention and treatment of bone-related issues. Developing a nutritional source of readily bioavailable calcium is particularly significant for individuals deficient in this essential element and at risk of developing osteoporosis. This research aimed to evaluate the impact of tempeh (T), daidzein (D), and Lactobacillus acidophilus (LA) within a simulated intestinal environment consisting of Caco-2 epithelial and Saos-2 cells, focusing on their implications for bone mineralization mechanisms. In the initial phase, calcium bioaccessibility from calcium citrate (CaCt), LA, D, the daidzein combination D–CaCt–LA (D1:1:1), and the tempeh combination T–CaCt–LA (T1:1:1) was assessed through digestion simulation. The calcium content of both untreated and digested samples was determined using atomic absorption spectrometry (AAS). In the subsequent stage, the digested samples were used to induce intestinal absorption in differentiated enterocyte-like Caco-2 cells. The permeable fractions were then evaluated in a culture of osteoblast-like Saos-2 cells. Preliminary cellular experiments employed the MTT assay to assess cytotoxicity. The results indicated that the analyzed products did not influence the deposition of extracellular calcium in Saos-2 cells cultured without mineralization stimulators. The combined formulations of permeable fractions of digested CaCt, LA, D, and T demonstrated the capacity to enhance the proliferation of Saos-2 cells. In Saos-2 cells, D, D1:1:1, and LA showed no discernible impact on intracellular calcium accumulation, whereas T and T1:1:1 reduced the calcium deposits. Additionally, mRNA transcripts and alkaline phosphatase (ALP) activity levels in Saos-2 cells cultured without mineralization induction were unaffected by the analyzed products. An examination of the products revealed no discernible effect on ALP activity or mRNA expression during Saos-2 cell differentiation. Our findings suggest that tempeh, daidzein, and L. acidophilus did not positively impact cellular calcium deposition in Saos-2 cells. However, tempeh, daidzein and its combination, and L. acidophilus might enhance the process of osteogenic differentiation in Saos-2 cells. Nevertheless, this study did not identify any synergistic impact on calcium deposition and the process of osteogenic differentiation in Saos-2 cells of isoflavones and probiotics.

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“…The addition of CPPs in culture medium increased soluble calcium concentration and enhanced calcification in the diaphyseal area of rudiments of embryonic rat bone . It is demonstrated that CPPs promoted calcium uptake of the differentiated HT‐29 and Caco2 cells . Meanwhile, CPPs increased calcium uptake and alkaline phosphatase (ALP) expression and activity of primary human osteoblast‐like cells .…”

Section: Introductionmentioning

confidence: 99%

Casein phosphopeptides-vaterite calcium carbonate enhance cytocompatibility and osteoinductivity of poly(l -lactic acid)

Liang

Chen

et al. 2014

Polym. Compos.

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Although calcium carbonate has been proved to be effective in neutralizing the acid degradation products of poly(L-lactic acid) (PLLA), it has no osteoinductivity. In this study, casein phosphopeptides (CPPs)-containing CaCO 3 vaterite were synthesized by fast precipitation in an aqueous solution of CaCl 2 , Na 2 CO 3 , and 2 mg/ml of CPPs. CaCO 3 -PLLA composite membranes (P-vaterite-CPP) were prepared (CaCO 3 /PLLA 51:2) and exhibited uniform structure and increased hydrophilicity. These composite membranes enhanced hydroxyapatite formation after incubated in simulated body fluid at 37 C for 7 days. The P-vaterite-CPP membranes promoted human bone marrow stromal cells (hBMSCs) proliferation 4 and 7 days after seeding. Scanning electron microscope images of hBMSCs on the composite membranes exhibited a polygonal and unevenly spreading morphology. Immunofluorescent staining of cytoskeleton and focal adhesion showed that hBMSCs had more stress fibers and were more spreading, indicating a good cell adhesion on the CPPs-containing composite membranes. Osteogenesis related genes (alkaline phosphatase, collagen type, osteocalcin, and osteopontin) expressions were significantly higher on the CPPs-containing membranes than those on the CPPs-free membranes (P < 0.05). It can be concluded that the addition of CPPs induces vaterite formation and thus improve cytocompatibility and osteoinductivity of PLLA.

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Calcium from LactoCalcium^TMMilk Mineral after Digestion with Pepsin Stimulates Mineralized Bone Nodule Formation in Human Osteoblast-Like SaOS-2 Cellsin Vitroand May Be Rendered Bioavailablein Vivo

Cited by 7 publications

References 41 publications

Application of in vitro bioaccessibility and bioavailability methods for calcium, carotenoids, folate, iron, magnesium, polyphenols, zinc, and vitamins B6, B12, D, and E

Application of in vitro bioaccessibility and bioavailability methods for calcium, carotenoids, folate, iron, magnesium, polyphenols, zinc, and vitamins B6, B12, D, and E

Effects of Daidzein, Tempeh, and a Probiotic Digested in an Artificial Gastrointestinal Tract on Calcium Deposition in Human Osteoblast-like Saos-2 Cells

Casein phosphopeptides-vaterite calcium carbonate enhance cytocompatibility and osteoinductivity of poly(l -lactic acid)

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