Application of in vitro bioaccessibility and bioavailability methods for calcium, carotenoids, folate, iron, magnesium, polyphenols, zinc, and vitamins B6, B12, D, and E

Etcheverry, Paz; Grusak, Michael A.; Fleige, Lisa

doi:10.3389/fphys.2012.00317

Cited by 297 publications

(288 citation statements)

References 193 publications

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“…This cell line derived from colonic adenocarcinoma surprisingly well mimics intestinal cell behaviour upon long culture. The fortnight culture of these cells on porous membranes of inserts leads to the creation of an impervious epithelial layer of polarized cells which resemble mature enterocytes (27). Tissue culture tests provide a relatively quick and cost-eff ective tool for predicting the absorption of bioactive components by assessing the probability of their transport through epithelial layer (28).…”

Section: Resultsmentioning

confidence: 99%

Phytases Improve Myo-Inositol Bioaccessibility in Rye Bread: A Study Using an In Vitro Method of Digestion and a Caco-2 Cell Culture Model

Duliński¹,

Cielecka²,

Pierzchalska³

et al. 2015

Food Technol. Biotechnol.

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SummaryPrepar ations of 6-phytase A (EC 3.1.3.26) and phytase B (acid phosphatase, EC 3.1.3.2) were applied alone and combined in the preparation of dough to estimate their catalytic potential for myo-inositol liberation from rye fl our in the breadmaking technology. The experimental bread samples were ground a er baking and subjected to determination of myo-inositol bioavailability by an in vitro method that simulated digestion in a human alimentary tract, followed by measurements of myo-inositol transport through enterocyte--like diff erentiated Caco-2 cells to determine its bioaccessibility. Myo-inositol content was measured by a high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) technique. The concentration of myo-inositol in the dialysates of control bread was 25.3 µg/mL, whereas in the dialysates of bread sample baked with 6-phytase A, the concentration increased to 35.4 µg/mL, and in the bread baked with phytase B to 64.98 µg/mL. Simultaneous application of both enzymes resulted in myo-inositol release of 64.04 µg/mL. The highest bioaccessibility of myo-inositol, assessed by the measurement of the passage through the Caco-2 monolayer was determined in the bread baked with the addition of 6-phytase A. Enzymatically modifi ed rye bread, particularly by the addition of 6-phytase A, may be therefore a rich source of a highly bioaccessible myo--inositol.

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Section: Resultsmentioning

confidence: 99%

Phytases Improve Myo-Inositol Bioaccessibility in Rye Bread: A Study Using an In Vitro Method of Digestion and a Caco-2 Cell Culture Model

Duliński¹,

Cielecka²,

Pierzchalska³

et al. 2015

Food Technol. Biotechnol.

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“…A limitation of in vitro iron solubility experiments is that they greatly oversimplify the highly complex physicochemical and physiologic events underlying human digestion (33). Thus, in vitro solubility may provide qualitative, but not quantitative, information on potential bioavailability (34) and measurements in vivo are more likely to mimic the true interactive effects of zinc on iron bioavailability from fortified rice (35).…”

Section: Discussionmentioning

confidence: 99%

Research on Natural Resources: The Quest for Integration Revisited

Bornemann¹,

Bernasconi²,

Ejderyan³

et al. 2017

GAIA - Ecological Perspectives for Science and Society

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The dysfunctional use of resources in modern societies arises from increasingly fragmented ways of understanding natural resources. Consequently, there have been repeated calls for integrative knowledge production, and various approaches to integration have been proposed, including such as interdisciplinary or transdisciplinary resource research and integrated resource management. The conceptual space proposed here can be applied as a tool for imagining and designing new approaches, methods, and research techniques toward a more reflexive understanding of integration-oriented research on natural resources.

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“…For example, the Mn bioaccessibility in the lettuce (Lactuca sativa L.) leaves decreased in the order of gastric > residual > intestinal [98], while [99] revealed that the solubility of Cd in the cock's comb (Celosia argentea L.) followed the order gastric > residual > intestinal. The bioaccessibility of metals from the different digestion phases is dependent on several factors such as the digestion and release of the ingested food matrix [100].…”

Section: First-second-third-fourth Phasesmentioning

confidence: 99%

Mn- and Cd-Contaminated Wild Water Spinach: in vitro Human Gastrointestinal Digestion Studies, Bioavailability Evaluation, and Health Risk Assessment

Guan¹,

Mohamat-Yusuff²,

Halimoon³

et al. 2018

Pol. J. Environ. Stud.

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Human health may be at risk when consuming edible but metal-contaminated aquatic plants. This present study was conducted to evaluate the bioavailabilities of manganese (Mn) and cadmium (Cd) in metalscontaminated wild water spinach (WWS), Ipomoea aquatic Forssk. through in vitro human gastrointestinal digestions. Additionally, the health risks from consuming the plant were also assessed. Metals-contaminated hydroponic nutrient solutions were used to grow the plants under greenhouse conditions. The plants were harvested after seven days of metal exposure and their edible shoots (stems and leaves) underwent digestions simulated from the human gastrointestinal tract. A standard reference material (peach leaves, SRM 1547) was used to assess the precision and accuracy of the in vitro digestion studies. Results showed that the metal concentrations in plants increased when the treatment concentration increased; the metals concentrations were higher in the raw (RHS) samples than in the cooked (DHS and CHS) samples. The bioavailabilities of Mn and Cd were found to be higher in the intestinal extractions than in the gastric extractions. The health risk index (HRI) showed that the adults averagely aged 44 in Selangor, Malaysia was at risk if they consumed Mn-T1-contaminated cooked (CHS) WWS and Cd-contaminated raw (RHS) and cooked (CHS) WWS at T1 and T2 because their HRI values were more than 1.

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Application of in vitro bioaccessibility and bioavailability methods for calcium, carotenoids, folate, iron, magnesium, polyphenols, zinc, and vitamins B6, B12, D, and E

Cited by 297 publications

References 193 publications

Phytases Improve Myo-Inositol Bioaccessibility in Rye Bread: A Study Using an In Vitro Method of Digestion and a Caco-2 Cell Culture Model

Phytases Improve Myo-Inositol Bioaccessibility in Rye Bread: A Study Using an In Vitro Method of Digestion and a Caco-2 Cell Culture Model

Research on Natural Resources: The Quest for Integration Revisited

Mn- and Cd-Contaminated Wild Water Spinach: in vitro Human Gastrointestinal Digestion Studies, Bioavailability Evaluation, and Health Risk Assessment

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