Calcium-Dependent Protein Kinase CPK1 Controls Cell Death by In Vivo Phosphorylation of Senescence Master Regulator ORE1

Durian, Guido; Sedaghatmehr, Mastoureh; Matallana-Ramirez, Lilian P.; Schilling, Silke; Schaepe, Sieke; Guerra, Tiziana; Herde, Marco; Witte, Claus-Peter; Mueller‐Roeber, Bernd; Schulze, Waltraud X.; Balazadeh, Salma; Romeis, Tina

doi:10.1105/tpc.19.00810

Cited by 39 publications

(60 citation statements)

References 62 publications

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“…54 independent A‐28/29 lines, 26 A‐29 lines and 27 A‐28 lines were obtained, and all of them grew normally, were phenotypically inconspicuous and indistinguishable from the wild type (Figure 2b). This is in contrast to CPKs involved in biotic stress responses, whose overexpression often results in mutant phenotypes such as stunted growth and/or formation of necrotic lesions (Dubiella et al ., 2013; Durian et al ., 2020) as a consequence of enhanced and prolonged stress signalling.…”

Section: Resultsmentioning

confidence: 99%

Improving plant drought tolerance and growth under water limitation through combinatorial engineering of signalling networks

Schulz

Piepenburg

Lintermann

et al. 2020

Plant Biotechnology Journal

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Agriculture is by far the biggest water consumer on our planet, accounting for 70 per cent of all freshwater withdrawals. Climate change and a growing world population increase pressure on agriculture to use water more efficiently ('more crop per drop'). Water-use efficiency (WUE) and drought tolerance of crops are complex traits that are determined by many physiological processes whose interplay is not well understood. Here, we describe a combinatorial engineering approach to optimize signalling networks involved in the control of stress tolerance. Screening a large population of combinatorially transformed plant lines, we identified a combination of calcium-dependent protein kinase genes that confers enhanced drought stress tolerance and improved growth under water-limiting conditions. Targeted introduction of this gene combination into plants increased plant survival under drought and enhanced growth under waterlimited conditions. Our work provides an efficient strategy for engineering complex signalling networks to improve plant performance under adverse environmental conditions, which does not depend on prior understanding of network function.

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Section: Resultsmentioning

confidence: 99%

Improving plant drought tolerance and growth under water limitation through combinatorial engineering of signalling networks

Schulz

Piepenburg

Lintermann

et al. 2020

Plant Biotechnology Journal

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“…MtCDPK4, 8, 15, 16, and 22 transcripts were quickly increased after 2 h of cold treatment. MtCDPK2 transcript was increased continuously under drought stress, MtCDPK4, 8,12,15,16,21, and 22 transcripts were increased and peaked after 2 h of drought treatment, whereas MtCDPK22 transcript was decreased at 6 h. In contrast, MtCDPK23 and MtCRK6 were downregulated after drought treatment. In response to salt treatment, MtCDPK2, 4, 8, 12, and 16 transcripts were upregulated after salt stress, while MtCRK6, MtCDPK7, and MtCDPK17 transcripts were downregulated (Figure 6A and Table S6).…”

Section: Expression Patterns Of Mtcdpk and Mtcrk Genes In Response To Abiotic And Biotic Stressesmentioning

confidence: 92%

Genome-Wide Identification and Characterization of Calcium-Dependent Protein Kinase (CDPK) and CDPK-Related Kinase (CRK) Gene Families in Medicago truncatula

Zhao

Liu

Kong

et al. 2021

IJMS

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Calcium-dependent protein kinase (CDPK or CPK) and CDPK-related kinase (CRK) play an important role in plant growth, development, and adaptation to environmental stresses. However, their gene families had been yet inadequately investigated in Medicago truncatula. In this study, six MtCRK genes were computationally identified, they were classified into five groups with MtCDPKs based on phylogenetic relationships. Six pairs of segmental duplications were observed in MtCDPK and MtCRK genes and the Ka/Ks ratio, an indicator of selection pressure, was below 0.310, indicating that these gene pairs underwent strong purifying selection. Cis-acting elements of morphogenesis, multiple hormone responses, and abiotic stresses were predicted in the promoter region. The spatial expression of MtCDPKs and MtCRKs displays diversity. The expression of MtCDPKs and MtCRKs could be regulated by various stresses. MtCDPK4, 14, 16, 22, and MtCRK6 harbor both N-myristoylation site and palmitoylation site and were anchored on plasma membrane, while MtCDPK7, 9, and 15 contain no or only one N-acylation site and were distributed in cytosol and nucleus, suggesting that the N-terminal acylation sites play a key role in subcellular localization of MtCDPKs and MtCRKs. In summary, comprehensive characterization of MtCDPKs and MtCRKs provide a subset of candidate genes for further functional analysis and genetic improvement against drought, cold, salt and biotic stress.

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“…CPK1-dependent phosphorylation of ORE1 increases transcriptional activation of the ORE1 downstream target gene BFN1, and plants overexpressing ORE1 , undergo early senescence. A C-terminal IDR of ORE1, containing eight serine and threonine residues, was suggested to be a phosphorylation hotspot [ 201 , 202 ] ( Figure 1 A). Multiple phosphorylations introduce multiple negative charges, which could affect the ORE1 IDR in many ways, as described above.…”

Section: Posttranslational Modifications Of Tf Idrsmentioning

confidence: 99%

Intrinsic Disorder in Plant Transcription Factor Systems: Functional Implications

Salladini

Jørgensen

Theisen

et al. 2020

IJMS

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Eukaryotic cells are complex biological systems that depend on highly connected molecular interaction networks with intrinsically disordered proteins as essential components. Through specific examples, we relate the conformational ensemble nature of intrinsic disorder (ID) in transcription factors to functions in plants. Transcription factors contain large regulatory ID-regions with numerous orphan sequence motifs, representing potential important interaction sites. ID-regions may affect DNA-binding through electrostatic interactions or allosterically as for the bZIP transcription factors, in which the DNA-binding domains also populate ensembles of dynamic transient structures. The flexibility of ID is well-suited for interaction networks requiring efficient molecular adjustments. For example, Radical Induced Cell Death1 depends on ID in transcription factors for its numerous, structurally heterogeneous interactions, and the JAZ:MYC:MED15 regulatory unit depends on protein dynamics, including binding-associated unfolding, for regulation of jasmonate-signaling. Flexibility makes ID-regions excellent targets of posttranslational modifications. For example, the extent of phosphorylation of the NAC transcription factor SOG1 regulates target gene expression and the DNA-damage response, and phosphorylation of the AP2/ERF transcription factor DREB2A acts as a switch enabling heat-regulated degradation. ID-related phase separation is emerging as being important to transcriptional regulation with condensates functioning in storage and inactivation of transcription factors. The applicative potential of ID-regions is apparent, as removal of an ID-region of the AP2/ERF transcription factor WRI1 affects its stability and consequently oil biosynthesis. The highlighted examples show that ID plays essential functional roles in plant biology and has a promising potential in engineering.

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Calcium-Dependent Protein Kinase CPK1 Controls Cell Death by In Vivo Phosphorylation of Senescence Master Regulator ORE1

Abstract: The calcium-dependent protein kinase CPK1 regulates a pivotal developmental switch in senescence and leaf cell death through direct phosphorylation of transcription factor ORE1.

Cited by 39 publications

References 62 publications

Improving plant drought tolerance and growth under water limitation through combinatorial engineering of signalling networks

Improving plant drought tolerance and growth under water limitation through combinatorial engineering of signalling networks

Genome-Wide Identification and Characterization of Calcium-Dependent Protein Kinase (CDPK) and CDPK-Related Kinase (CRK) Gene Families in Medicago truncatula

Intrinsic Disorder in Plant Transcription Factor Systems: Functional Implications

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