Intrinsic Disorder in Plant Transcription Factor Systems: Functional Implications

Salladini, Edoardo; Jørgensen, Maria Louise Mønster; Theisen, Frederik Friis; Skriver, Karen

doi:10.3390/ijms21249755

Cited by 18 publications

(14 citation statements)

References 271 publications

(466 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intrinsically disordered regions (IDRs) of proteins are biologically active and their presence facilitates the versatile protein–protein interactions networks that are involved in signalling pathways to counteract stress effects (Salladini et al, 2020). Hence, we analyzed CaPDZ1 for the presence of IDRs.…”

Section: Resultsmentioning

confidence: 99%

Dehydration‐responsive chickpea chloroplast protein, CaPDZ1, confers dehydration tolerance by improving photosynthesis

Lande

Barua

Gayen

et al. 2022

Physiologia Plantarum

View full text Add to dashboard Cite

The screening of a dehydration‐responsive chloroplast proteome of chickpea led us to identify and investigate the functional importance of an uncharacterized protein, designated CaPDZ1. In all, we identified 14 CaPDZs, and phylogenetic analysis revealed that these belong to photosynthetic eukaryotes. Sequence analyses of CaPDZs indicated that CaPDZ1 is a unique member, which harbours a TPR domain besides a PDZ domain. The global expression analysis showed that CaPDZs are intimately associated with various stresses such as dehydration and oxidative stress along with certain phytohormone responses. The CaPDZ1‐overexpressing chickpea seedlings exhibited distinct phenotypic and molecular responses, particularly increased photosystem (PS) efficiency, ETR and qP that validated its participation in PSII complex assembly and/or repair. The investigation of CaPDZ1 interacting proteins through Y2H library screening and co‐IP analysis revealed the interacting partners to be PSII associated CP43, CP47, D1, D2 and STN8. These findings supported the earlier hypothesis regarding the role of direct or indirect involvement of PDZ proteins in PS assembly or repair. Moreover, the GUS‐promoter analysis demonstrated the preferential expression of CaPDZ1 specifically in photosynthetic tissues. We classified CaPDZ1 as a dehydration‐responsive chloroplast intrinsic protein with multi‐fold abundance under dehydration stress, which may participate synergistically with other chloroplast proteins in the maintenance of the photosystem.

show abstract

Section: Resultsmentioning

confidence: 99%

Dehydration‐responsive chickpea chloroplast protein, CaPDZ1, confers dehydration tolerance by improving photosynthesis

Lande

Barua

Gayen

et al. 2022

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…In most eukaryotes, >80% of transcription factors and 75% of Mediator subunits possess extended (≥30 residues) IDRs ( Liu et al, 2006 ; Tóth-Petróczy et al, 2008 ). Similarly, numerous plant transcription factors and Mediator subunits are predicted to contain at least one IDR ( Nagulapalli et al, 2016 ; Salladini et al, 2020 ). In particular, the bHLH transcription factor family, to which PIF4 belongs, is hypothesized to have a general ability to undergo spontaneous liquid–liquid phase separation in transcription regulation by their IDRs ( Tarczewska and Greb-Markiewicz, 2019 ; Salladini et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

PHYTOCHROME-INTERACTING FACTOR 4/HEMERA-mediated thermosensory growth requires the Mediator subunit MED14

Bajracharya

Grace

et al. 2022

Plant Physiology

View full text Add to dashboard Cite

While moderately elevated ambient temperatures do not trigger stress responses in plants, they do substantially stimulate the growth of specific organs through a process known as thermomorphogenesis. The basic helix-loop-helix transcription factor PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) plays a central role in regulating thermomorphogenetic hypocotyl elongation in various plant species, including Arabidopsis (Arabidopsis thaliana). Although it is well known that PIF4 and its co-activator HEMERA (HMR) promote plant thermosensory growth by activating genes involved in the biosynthesis and signaling of the phytohormone auxin, the detailed molecular mechanism of such transcriptional activation is not clear. In this report, we investigated the role of the Mediator complex in the PIF4/HMR-mediated thermoresponsive gene expression. Through the characterization of various mutants of the Mediator complex, a tail subunit named MED14 was identified as an essential factor for thermomorphogenetic hypocotyl growth. MED14 was required for the thermal induction of PIF4 target genes but had a marginal effect on the levels of PIF4 and HMR. Further transcriptomic analyses confirmed that the expression of numerous PIF4/HMR-dependent, auxin-related genes required MED14 at warm temperatures. Moreover, PIF4 and HMR physically interacted with MED14 and both were indispensable for the association of MED14 with the promoters of these thermoresponsive genes. While PIF4 did not regulate MED14 levels, HMR was required for the transcript abundance of MED14. Taken together, these results unveil an important thermomorphogenetic mechanism, in which PIF4 and HMR recruit the Mediator complex to activate auxin-related growth-promoting genes when plants sense moderate increases in ambient temperature.

show abstract

“…In most eukaryotes, more than 80% of transcription factors and 75% of Mediator subunits possess extended (≥30 residues) IDRs (Liu et al, 2006; Tóth-Petróczy et al, 2008). Similarly, numerous plant transcription factors and Mediator subunits are predicted to contain at least one IDR (Nagulapalli et al, 2016; Salladini et al, 2020). In particular, the bHLH transcription factor family, to which PIF4 belongs, is hypothesized to have a general ability to undergo spontaneous liquid-liquid phase separation in transcription regulation by their IDRs (Tarczewska and Greb-Markiewicz, 2019; Salladini et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

PIF4/HEMERA-mediated daytime thermosensory growth requires the Mediator subunit MED14

Qiu

Bajracharya

et al. 2022

Preprint

View full text Add to dashboard Cite

While moderately elevated ambient temperatures do not trigger stress responses in plants, they do significantly stimulate the growth of specific organs through a process known as thermomorphogenesis. The basic helix-loop-helix transcription factor PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) plays a central role in regulating thermomorphogenetic hypocotyl elongation in various plant species, including Arabidopsis thaliana. Although it is well known that PIF4 promotes plant thermosensory growth by activating genes involved in the biosynthesis and signaling of the phytohormone auxin, the detailed molecular mechanism of such transcriptional activation is not clear. Our previous studies demonstrated that HEMERA (HMR), a transcriptional co-activator of PIF4, promotes the thermo-induced expression of PIF4 target genes through its nine-amino-acid transactivation domain (9aaTAD). In this report, we investigate the role of the Mediator complex in the PIF4/HMR-mediated thermoresponsive gene expression. Through the characterization of various mutants of the Mediator complex, a tail subunit named MED14 is identified as an essential factor for thermomorphogenetic hypocotyl growth. MED14 is required for the thermal induction of PIF4 target genes but has a marginal effect on the levels of PIF4 and HMR. Further transcriptomic analyses confirm that the expression of numerous PIF4/HMR-dependent, auxin-related genes requires MED14 at warm temperatures. Moreover, PIF4 and HMR physically interact with MED14 and both are indispensable for the association of MED14 with the promoters of these thermoresponsive genes. Taken together, these results unveil an important thermomorphogenetic mechanism, in which PIF4 and HMR recruit the Mediator complex to activate auxin-related growth-promoting genes when plants sense moderate increases in ambient temperature.One-sentence summaryThe Mediator subunit MED14 promotes thermomorphogenesis by interacting with PHYTOCHROME-INTERACTING FACTOR 4 and HEMERA to induce the expression of growth-promoting genes at elevated temperatures.

show abstract

Intrinsic Disorder in Plant Transcription Factor Systems: Functional Implications

Cited by 18 publications

References 271 publications

Dehydration‐responsive chickpea chloroplast protein, CaPDZ1, confers dehydration tolerance by improving photosynthesis

Dehydration‐responsive chickpea chloroplast protein, CaPDZ1, confers dehydration tolerance by improving photosynthesis

PHYTOCHROME-INTERACTING FACTOR 4/HEMERA-mediated thermosensory growth requires the Mediator subunit MED14

PIF4/HEMERA-mediated daytime thermosensory growth requires the Mediator subunit MED14

Contact Info

Product

Resources

About