Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress

Abstract

Calcium-dependent protein kinases (CDPK) are implicated in signaling transduction in eukaryotic organisms. It is largely unknown whether StCDPK28 plays a role in the response to water deficiency and osmotic stress in potato plants (Solanum tuberosum L.). Potato cv. Zihuabai was cultivated under natural, moderate, and severe water deficiency conditions; to induce osmotic stress, potato plants were treated with 10% or 20% PEG. StCDPK28-overexpression and StCDPK28-knockdown plants were constructed. StCDPKs were evaluated by qRT-PCR. The subcellular location of the StCDPK28 protein was observed with confocal scanning laser microscopy. Phenotypic changes were indicated by photosynthetic activity, the contents of H2O2, MDA and proline, and the activities of CAT, SOD and POD. Results showed water deficiency and osmotic stress altered StCDPK expression patterns. StCDPK28 exhibited a membrane, cytosolic and nuclear localization. Water deficiency and osmotic stress induced StCDPK28 upregulation. Photosynthetic activity was enhanced by StCDPK28 overexpression, while decreased by StCDPK2 knockdown under water deficiency and osmotic stress. StCDPK28 overexpression decreased H2O2 and MDA, and increased proline, while StCDPK28 knockdown showed reverse results, compared with the wild type, in response to water deficiency and osmotic stress. StCDPK28 overexpression increased the activities of CAT, SOD and POD, while StCDPK28-knockdown plants indicated the reverse trend under water deficiency and osmotic stress conditions. Regulation of StCDPK28 expression could be a promising approach to improve the tolerance ability of potato plants in response to drought or high salt media.

Genome-wide characterization of SOS1 gene family in potato (Solanum tuberosum) and expression analyses under salt and hormone stress

“…It was reported that soil salinization negatively affected the growth and yield of potato crops, especially in arid and semi-arid climates (Li et al, 2022), which caused osmotic and oxidative stress, ion imbalance, mineral deficiency, and ion toxicity problems (Hamooh et al, 2021). Therefore, the selection and breeding of salt-tolerant genes has become a promising approach for improving the yield and adaptability of potato (Zhu et al, 2022). Previous studies have shown that a gene encoding SOS2 (PGSC0003DMG400006384) is up-regulated, indicating that this gene plays an active regulatory role in salt stress response.…”

CBL-Interacting Protein Kinases 18 (CIPK18) Gene Positively Regulates Drought Resistance in Potato

“…After 2 days, the microtuber slices were further cultured on a new solid MS medium at 25 °C, 2500 Lx ray, and the medium was changed once a week [ 39 ]. When the differentiated shoots reached 1–1.5cm, they were cut and moved to the rooting medium containing kanamycin (75 mg/L) or hygromycin (50 mg/L) and carbenmycin (200 mg/L), respectively, for rooting screening [ 40 ] using the CTAB method to extract the DNA of transgenic plants that could root normally. Non-transferred plants were used as a negative control and empty vector pCAMBIA1300-35S-EGFP was used as a positive control.…”