Sensor—responder complexes comprising calcineurin B-like (CBL) proteins and CBL-interacting protein kinases (CIPKs) are plant-specific Ca2+ receptors, and the CBL-CIPK module is widely involved in plant growth and development and a large number of abiotic stress response signaling pathways. In this study, the potato cv. “Atlantic” was subjected to a water deficiency treatment and the expression of StCIPK18 gene was detected by qRT-PCR. The subcellular localization of StCIPK18 protein was observed by a confocal laser scanning microscope. The StCIPK18 interacting protein was identified and verified by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC). StCIPK18 overexpression and StCIPK18 knockout plants were constructed. The phenotypic changes under drought stress were indicated by water loss rate, relative water content, MDA and proline contents, and CAT, SOD and POD activities. The results showed that StCIPK18 expression was upregulated under drought stress. StCIPK18 is localized in the cell membrane and cytoplasm. Y2H shows the interaction between StCIPK18 and StCBL1, StCBL4, StCBL6 and StCBL8. BiFC further verifies the reliability of the interaction between StCIPK18 and StCBL4. Under drought stress, StCIPK18 overexpression decreased the water loss rate and MDA, and increased RWC, proline contents and CAT, SOD and POD activities; however, StCIPK18 knockout showed opposite results, compared with the wild type, in response to drought stress. The results can provide information for the molecular mechanism of the StCIPK18 regulating potato response to drought stress.
Lilium species with ornamental, edible, and medicinal values are distributed all over the world. Little is known about the responses of Lilium genotypes to waterlogging stress. Lilium hybrid 'Brindisi' was used to study the physiological responses of roots, bulbs, and leaves to 1, 2, 4, 8, and 13 d of waterlogging stress. Results showed that waterlogging stress seriously hindered the transport of nutrients from bulbs to stems and leaves. The physiological indicators could be divided into two categories. The first category was the physiological parameters indicating plant damage. The dry and fresh masses of stems and leaves, chlorophyll (Chl) a, Chl b, Chl a+b, and carotenoid content decreased, the dry and fresh masses of bulbs, malondialdehyde and H 2O2 content increased under waterlogging stress. The other category was the physiological indicators that regulate the plant adaptability to waterlogging stress. Among them, superoxide dismutase and pyruvate decarboxylase activity changed little, proline content increased significantly, soluble sugar and protein content, and ascorbate peroxidase (APX), catalase (CAT), alcohol dehydrogenase, and lactate dehydrogenase activities increased in the early stage, and decreased in the later stage of waterlogging stress. The turning point of these physiological parameters was 4 -8 d after waterlogging stress. Bulbs played an important role in alleviating flooding stress in the early stage of waterlogging. APX and CAT also played an important role in eliminating ROS in the early stage. This research lays foundation for the research on the mechanism of waterlogging tolerance and breeding of waterlogging-tolerant cultivars of Lilium spp.
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