Calcium chloride‐enriched calcium aluminate cement promotes <i>in vitro</i> osteogenesis

Castro‐Raucci, Larissa Moreira Spinola de; Teixeira, Lucas Novaes; Barbosa, Ana Flávia Simões; Fernandes, Roger Rodrigo; Raucci-Neto, Walter; Jacobovitz, Marcos; Oliveira, I. R.; Oliveira, Paulo Tambasco de

doi:10.1111/iej.12883

Cited by 11 publications

(14 citation statements)

References 41 publications

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“…Therefore, calcium deposits are an indication of successful in vitro bonelike nodule formation that can specifically be stained bright orange-red using Alizarin Red S dye. The results showed that ZnO addition in CACH significantly inhibited matrix mineralization, and these findings agree with the observations of Togari et al (1993) and Vicuna et al (2019) showing that zinc exposure reduces calcium content and inhibits matrix mineralization in osteogenic cell cultures, yet it induces alkaline phosphatase activity and gene expression of osteoblast markers (Togari et al 1993;Castro-Raucci et al 2018;Vicuna et al 2019).…”

Section: Discussionsupporting

confidence: 86%

Calcium aluminate cement-based blends for application to fill in bone defects

et al. 2020

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Introduction The need to treat bone defects is increasing dramatically as the population grows old. Calcium aluminate cement, CAC, has been applied to fill in bone defects. CAC has been used in orthopedics, as it can avoid some problems of commercial products, polymethylmethacrylate, PMMA, regarding its too low consistency and strong odor and exothermic reactions which can damage the surrounding tissue. Besides that, CAC can be molded in situ to provide intimate fitting to the defect rims. The injectability of cements is an important aspect when minimally invasive surgical techniques are used and for applications with limited accessibility and narrow cavities. Methods At present work, CAC was initially analyzed via zeta potential and viscosity when mixed with different dispersant additives. Then, CAC blends containing different materials, alumina, zirconia, zinc oxide, hydroxyapatite, tricalcium phosphate, chitosan, collagen, or zinc oxide-bismuth oxide, were evaluated regarding their injectability and by means of in vitro mineralization and ex vivo uniaxial cold crushing strength tests. The best injectability conditions, 76 wt% solids, hypodermic needle, were used for the ex vivo tests. In the latter, the compositions selected by in vitro tests were injected to repair 3-mm defects on mouse femurs cut into pieces of 10 mm, whereas collagen and PMMA were inserted with a spatula. After setting the materials for 24 h at 37°C in body fluid solution, the pieces were submitted to uniaxial cold crushing strength tests. Results The blends alumina, collagen, and chitosan resulted in greater reddish-stained areas indicating enhanced mineralized matrix formation for these groups, which also presented higher values of calcium deposits indicating a successful in vitro bonelike nodule formation. Conclusion The filling of defects with CACH and its blends increased the resistance of bones when compared to bones with the added defect, with lower effect verified in the presence of chitosan and collagen.

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Section: Discussionsupporting

confidence: 86%

Calcium aluminate cement-based blends for application to fill in bone defects

et al. 2020

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“…The term "hydraulic" is used to define the setting reaction (i.e., with water), and the ability to set and then be stable in a wet environment, which is crucial. For this reason, a sub-classification of hydraulic silicate cements has been used to differentiate materials such as Portland cement, which is a silicate, from calcium aluminates that are also being proposed as dental materials (17)(18)(19). These materials are all hydraulic but have different chemistries otherwise.…”

Section: Discussionmentioning

confidence: 99%

Classification of Hydraulic Cements Used in Dentistry

Camilleri

2020

Front. Dent. Med

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Since their introduction in clinical dentistry, hydraulic cements have gained popularity. Their applications are diverse and their usefulness is due to their hydraulic nature. These materials require water to set and reach their optimal physical and mechanical characteristics, do not deteriorate when wet, and form calcium hydroxide as a by-product of the hydration reaction. All these characteristics are important for a number of clinical applications. The first hydraulic cement was a simple mixture of Portland cement, as used in the construction industry, with bismuth oxide to increase its radio-opacity. Regardless of being a hydraulic calcium silicate, it was initially incorrectly labeled as a phosphate cement misleadingly called "mineral trioxide aggregate." Since then, beneficial clinical applications have led to the development of a number of materials with a different base, alternative vehicles, and incorporating modifiers of various kinds. Given the variety, and possible confusion, a rational classification of hydraulic cements used in dentistry is necessary. The classification is primarily dependent on the clinical context in which the materials are used as this informs the user about the environment and possible interactions. Secondly, a classification based on the material constitution is given as knowledge of its chemistry will help predict behavior, identify risks, and thus facilitate selection and handling.

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“…The following cements were used: 1) CACz 15 , 18 ; 2) CACb with Bi 2 O 3 and 2.8% CaCl 2 ; 3) CACb+ with Bi 2 O 3 and 10% CaCl 2 ; and 4) MTA (Angelus, Londrina, PR, Brazil). The CAC powders were mixed with sterile water (3:1, v/v).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Aiming to improve the biological and clinical properties of CACz, we proposed raising the CaCl 2 content to increase calcium release and enhancing its radiopacity by replacing ZnO by Bi 2 O 3 17 , 18 . In osteoblastic cells, CAC with Bi 2 O 3 and increased CaCl 2 content supported cell differentiation and matrix mineralization 18 . However, given that cell responses to a biomaterial might vary depending on the cell type and/or its differentiation stage, 19 , 20 assessing the odontoblast response to the formulations of CAC proposed is much needed, aiming at defining the suitability of this material for dentin-pulp complex repair therapies.…”

Section: Introductionmentioning

confidence: 99%

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Impact of calcium aluminate cement with additives on dental pulp-derived cells

et al. 2020

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Calcium aluminate cement (CAC) has been highlighted as a promising alternative for endodontic use aiming at periapical tissue repair. However, its effects on dental pulp cells have been poorly explored. Objective: This study assessed the impact of calcium chloride (CaCl2) and bismuth oxide (Bi2O3) or zinc oxide (ZnO) additives on odontoblast cell response to CAC. Methodology: MDPC-23 cells were exposed for up to 14 d: 1) CAC with 2.8% CaCl2 and 25% ZnO (CACz); 2) CAC with 2.8% CaCl2 and 25% Bi2O3 (CACb); 3) CAC with 10% CaCl2 and 25% Bi2O3 (CACb+); or 4) mineral trioxide aggregate (MTA), placed on inserts. Non-exposed cultures served as control. Cell morphology, cell viability, gene expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), and dentin matrix protein 1 (DMP-1), ALP activity, and extracellular matrix mineralization were evaluated. Data were compared using ANOVA (α=5%). Results: Lower cell density was detected only for MTA and CACb+ compared with Control, with areas showing reduced cell spreading. Cell viability was similar among groups at days one and three (p>0.05). CACb+ and MTA showed the lowest cell viability values at day seven (p>0.05). CACb and CACb+ promoted higher ALP and BSP expression compared with CACz (p<0.05); despite that, all cements supported ALP activity. Matrix mineralization were enhanced in CACb+ and MTA. Conclusion: In conclusion, CAC with Bi2O3, but not with ZnO, supported the expression of odontoblastic phenotype, but only the composition with 10% CaCl2 promoted mineralized matrix formation, rendering it suitable for dentin-pulp complex repair.

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Calcium chloride‐enriched calcium aluminate cement promotes in vitro osteogenesis

Cited by 11 publications

References 41 publications

Calcium aluminate cement-based blends for application to fill in bone defects

Calcium aluminate cement-based blends for application to fill in bone defects

Classification of Hydraulic Cements Used in Dentistry

Impact of calcium aluminate cement with additives on dental pulp-derived cells

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