Calcium carbonate mineralization mediated by in vitro cultured mantle cells from Pinctada fucata

Amphiphilic Copolymer-Modified Eggshell-Based Column Packing Materials for the Preparative Separation of Basic Drugs

“…Ethylenediaminetetraacetic acid (EDTA) was effectively used to dissolve the surface of calcium carbonate crystals because of its selective chelating ability for calcium ions. , Therefore, eggshell powder was first surface-treated with buffered EDTA solution under nine different reaction conditions to maximize the porous structure that eggshells originally possessed (Figure S1). At concentrations of 250 and 500 mM of EDTA, expansion of the eggshell pores was observed, resulting in particles with a uniform porous structure.…”

Recent Advances of Shell Matrix Proteins and Cellular Orchestration in Marine Molluscan Shell Biomineralization

“…The N-terminus of the laminin G domain is present between the chitin-binding domain and the C-terminus of Pif 97, while the C-terminus of the laminin G is located at the center of Pif 80, which may contribute to the CaCO 3 -binding activity of Pif (Suzuki et al, 2013). In C. gigas, the morphology of the (Wang et al, 2013a;Kong et al, 2015;Li et al, 2016). The notes for some of the shapes included in this figure are shown on the upper left.…”

“…Secretome and transcriptome data reveal that some SMPs are non-secretory, and some others were abundant in other tissues (Marie et al, 2010(Marie et al, , 2012Kinoshita et al, 2011;Mann et al, 2012;Wang et al, 2013a). Furthermore, biominerals and SMPs are observed in the hemocytes (Johnstone et al, 2008;Calvoiglesias et al, 2016), and hemocytes as well as OME assembling on the surface of regenerated prismatic layer Johnstone et al, 2015;Kong et al, 2015), prompt us to investigate the mechanism of shell formation from cellular aspects. The hypothesis that co-operative interaction of hemocytes and OME during shell formation have been developed (Figure 1).…”

“…One alternative to the matrix-mediated hypothesis is the cell-mediated hypothesis, which proposes that crystal nucleation occurs in hemocytes or OME, and that crystal-bearing cells transport nascent crystals intracellularly to the mineralization front (Mount et al, 2004;Gong et al, 2008a;Xiang et al, 2014). Although the cellular basis has been reported in many other biomineralization, such as osteoclasts and primary mesenchyme cells involved in bone and spicule formation in vertebrates and echinoderms, respectively (Wilt, 2002;Kylmaoja et al, 2016), this hypothesis still largely scraps the dominant paradigm in molluscan biomineralization, and has been supported by increasing evidence, which will be introduced in the following text (Mount et al, 2004;Fleury et al, 2008;Johnstone et al, 2008Johnstone et al, , 2015Kong et al, 2015;Li et al, 2016). To date, cells coming from two different sources have been observed involved in shell formation: (1) OMEs mediate shell formation by either directly involving in the nucleation and remodeling process of CaCO 3 mineral (Kong et al, 2015) or secreting the organic matrix, among which shell matrix proteins (SMPs) regulate the diversity of shell shapes by orchestrating the CaCO 3 crystals in a specific manner (Lowenstam and Weiner, 1989;Zhang and Zhang, 2006;Marin et al, 2008;Ivanina et al, 2017); and (2) hemocytes participate in the deposition of intracellular CaCO 3 crystals and deliver them to the mineralization site (Mount et al, 2004;Fleury et al, 2008;Kádár, 2008;Mount and Pickering, 2009;Johnstone et al, 2015;Li et al, 2016;Ivanina et al, 2017).…”

Transcriptome analysis of the bivalve Placuna placenta mantle reveals potential biomineralization-related genes

“…Most of matrix proteins in molluscan shells are specifically secreted by the mantle tissue of molluscans. In another word, the proteins from the mantle tissues of molluscans can tune the formation of different structural layers of the shells 12 , 21 , 22 . It was considered that the shell matrix proteins extracted from skeletons or shells generally have high expression in mantle tissues.…”