Calcium carbonate microparticles with embedded silver and magnetite nanoparticles as new SERS-active sorbent for solid phase extraction

Markina, Natalia E.; Маркин, А. В.; Zakharevich, Andrey M.; Goryacheva, Irina Yu.

doi:10.1007/s00604-017-2426-6

Cited by 29 publications

(9 citation statements)

References 27 publications

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“…A high loading of proteins into the crystals will also ensure a high loading capacity for the carriers assembled using the crystal. We note that other applications of the crystals including sensors 67,68 or self-assembled tailor-made polymer macrostructures such as scaffolds [69][70][71] have been reported.…”

Section: -33mentioning

confidence: 99%

The mechanism of catalase loading into porous vaterite CaCO₃ crystals by co-synthesis

Vikulina

Feoktistova

Балабушевич

et al. 2018

Phys. Chem. Chem. Phys.

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Porous vaterite CaCO 3 crystals are nowadays extensively used as high-capacity bio-friendly sacrificial templates for the fabrication of such protein-containing nano-and micro-particles as capsules and beads. The first step in the protein encapsulation is performed through loading of the protein molecules into the crystals. Co-synthesis is one of the most useful and simple methods proven to effectively load crystals with proteins; however, the loading mechanism is still unknown. To understand the mechanism, in this study, we focus on the loading of a model protein catalase into the crystals by means of adsorption into pre-formed crystals (ADS) and co-synthesis (COS). Analysis of the physico-chemical characteristics of the protein in solution and during the loading and simulation of the protein packing into the crystals are performed. COS provides more effective loading than ADS giving protein contents in the crystals of 20.3 and 3.5 w/w%, respectively. Extremely high loading for COS providing a local protein concentration of about 550 mg mL À1 is explained by intermolecular protein interactions, i.e. by adsorption of the aggregates into the crystals. We believe that this study will be useful for protein encapsulation through CaCO 3 crystals using the COS method.

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Section: -33mentioning

confidence: 99%

The mechanism of catalase loading into porous vaterite CaCO₃ crystals by co-synthesis

Vikulina

Feoktistova

Балабушевич

et al. 2018

Phys. Chem. Chem. Phys.

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“…Additionally, the protocol can be further improved by adjustment of the sorbent characteristics using modification during the synthesis. For example, the addition of polyelectrolyte molecules improves the sorption of the regarding the charged analytes [16,20,31]. Lastly, the amphoteric properties of AO also enable the sorbent dissolution by acids that is suitable for detection of sulfonamides and some other drugs which possess strong SERS signal in acidic media without overlap with the signal of CRN [25,32].…”

Section: Comparison With Other Sers-based Approachesmentioning

confidence: 99%

“…These substrates improve colloidal and chemical stability of the nanoparticles and possess better sorption capabilities compared with the pure nanoparticles [13,14]. For example, the improvement of limits of detection up to several orders has been demonstrated in several reports including ours [15,16]. This effect was achieved due to solid-phase extraction (SPE) of analyte molecules by porous SERS substrate with further detection directly in the substrate.…”

Section: Introductionmentioning

confidence: 99%

Determination of methotrexate in spiked human urine using SERS-active sorbent

2020

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This report is dedicated to determination of anticancer drug methotrexate (MTX) in human urine using surface-enhanced Raman spectroscopy (SERS). Aluminum oxide loaded with silver nanoparticles (AO-Ag) was proposed as SERS-active sorbent and used for solid-phase extraction (SPE) of the analyte and its SERS-based determination (SPE-SERS protocol). MTX has strong SERS signal only in alkaline media that challenges its determination in urine due to strong background signal caused by creatinine. The application of SPE step enables to purify and concentrate the analyte making MTX determination possible. Also, the application of the same material for SPE pretreatment and SERS analysis enables to simplify and speed-up the protocol. The protocol was developed and tested using artificially spiked samples of human urine collected during different time of day to account deviating composition of the urine matrix. The use of dilution step of the analyte-containing urine was proposed prior SPE-SERS protocol to reduce the difference between morning-time-and daytime-collected urine achieving maximal reliability of the analysis. Additional physicochemical study was performed to estimate an influence of the primary intrinsic urine components (salts, urea, creatinine) and their mixtures on the analytical signal. Final protocol enables MTX determination in human urine within 20-300 μg mL −1 range of concentrations with satisfactory precision (11-19% RSD), accuracy (97-104% apparent recovery), and limit of detection (4.2 μg mL −1). Accounting that the analysis requires less than 15 min and portable Raman spectrometer, the protocol seems to be promising for therapeutic drug monitoring in hospitals to identify poor MTX clearance in a timely manner and minimize adverse effects of therapy.

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“…The NDs' size and density are related to the ITO film thickness and the reaction kinetics [23]. In this work, the effective factors of reaction time, applied voltage, and ITO film thickness were all investigated to find out the ND formation process.…”

Section: Electrolysis Parameters: Reaction Time Applied Voltage Andmentioning

confidence: 99%

Large-Area and Patternable Nano-Dot Array from Electrolysis of ITO Film for Surface-Enhanced Raman Spectroscopy

et al. 2020

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Fabrication of large-area devices with patternable nanostructures is important for practical applications in optical or electrical devices. In this work, we describe an easy and environment-friendly method for preparing large-area nano-dot (ND) arrays via the electrolytic reaction of a metal oxide film. NDs with various size and morphology can be obtained by adjusting the applied voltage, electrolysis time, and the film thickness of the indium tin oxide (ITO) layer. High-density NDs with size of 50-60 nm can be obtained by electrolysis of a 25-nm-thick ITO film at 150 V for 1.5 min under a water droplet medium, which have been applied for surface-enhanced Raman spectroscopy (SERS) after depositing a thin layer of silver. The SERS substrate with optimized ND structure exhibits sensitive detection of Rhodamine 6G (R6G) with detection limit down to 5 × 10-12 M. The enhancement factors (EFs) of 1.12 × 10 6 and 6.79 × 10 5 have been achieved for characterization of 4-methylbenzenethiol (4-MBT) and R6G, respectively. With an additional photolithographic step, multiple areas of ND arrays can be created on one substrate, enabling simultaneous detection of various samples containing different molecules at once experiment. Such a method is quick, easy, patternable, and environment-friendly, being suitable for on-site quick and synchronous determination of various molecules for applications in point-of-care, environmental monitoring, and airport security fields.

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Calcium carbonate microparticles with embedded silver and magnetite nanoparticles as new SERS-active sorbent for solid phase extraction

Cited by 29 publications

References 27 publications

The mechanism of catalase loading into porous vaterite CaCO₃ crystals by co-synthesis

The mechanism of catalase loading into porous vaterite CaCO₃ crystals by co-synthesis

Determination of methotrexate in spiked human urine using SERS-active sorbent

Large-Area and Patternable Nano-Dot Array from Electrolysis of ITO Film for Surface-Enhanced Raman Spectroscopy

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Calcium carbonate microparticles with embedded silver and magnetite nanoparticles as new SERS-active sorbent for solid phase extraction

Cited by 29 publications

References 27 publications

The mechanism of catalase loading into porous vaterite CaCO3 crystals by co-synthesis

The mechanism of catalase loading into porous vaterite CaCO3 crystals by co-synthesis

Determination of methotrexate in spiked human urine using SERS-active sorbent

Large-Area and Patternable Nano-Dot Array from Electrolysis of ITO Film for Surface-Enhanced Raman Spectroscopy

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The mechanism of catalase loading into porous vaterite CaCO₃ crystals by co-synthesis

The mechanism of catalase loading into porous vaterite CaCO₃ crystals by co-synthesis