Calcium butyrate: Anti-inflammatory effect on experimental colitis in rats and antitumor properties

Abstract

Butyric acid is a physiological component of the colonic environment that possesses anti-inflammatory and antitumor properties, among others. However, little is known regarding its effects following direct application on the colonic surface. This study was conducted to investigate the topical anti-inflammatory effect of calcium butyrate in chemically-induced colitis in rats and to evaluate its antitumor properties and. The anti-inflammatory activity of calcium butyrate was evaluated in dinitrobenzene sulfonic acid-induced colitis in rats, following intracolonic instillation for 6 consecutive days and its antitumor activity was evaluated in F344 rats with the azoxymethane (AOM)-induced aberrant crypt foci (AFC) test, following intracolonic instillation for 4 weeks. The antiproliferative activity was assessed by incubation for 48 h with the HT29, SW620 and HCT116 intestinal tumour cell lines to evaluate the rate of H-thymidine uptake. In dinitrobenzene-induced colitis, the intracolonic instillation of calcium butyrate completely prevented body weight reduction in the animals and counteracted the local noxious effects of the irritant by reducing colon edema (-22.7%, P=0.048) and the area of mucosal damage (-48%, P=0.045). In the AOM-induced AFC test, the intracolonic instillation of calcium butyrate significantly reduced the number of AFC in the entire colon (-22.7%, P<0.05). Calcium butyrate, following incubation with the HT29, SW620 and HCT116 tumour cell lines, induced a significant antiproliferative, dose-dependent effect (P=0.046 to P=0.002) in all three strains, as measured by the reduction in H-thymidine uptake. Calcium butyrate directly applied to the mucosa of the rat colon was able to ameliorate colonic inflammation, suggesting a possible beneficial role in the treatment of inflammatory colon diseases. Moreover, calcium butyrate exhibited notable antitumor effects and ; however, their clinical relevance requires confirmation by additional clinical investigations.

Intestinal Microbiota of Broiler Chickens As Affected by Litter Management Regimens

“…Butyrate is anti-inflammatory (Van Immerseel et al, 2010 ; Celasco et al, 2014 ), and some of the identified cecal bacteria were assigned to known butyrate-producing genera, such as Faecalibacterium, Subdoligranulum , and Butyricicoccus (Louis and Flint, 2009 ). Faecalibacterium was more predominant in the cecal digesta of reused-litter chickens, while Subdoligranulum showed the opposite trend at both ages (Table 3 ).…”

Independent associations of dairy and calcium intakes with colorectal cancers in the Adventist Health Study-2 cohort

“…Lipid components of dairy products, including butyric acid and CLA, may protect against tumorigenesis ( 40 ) . Butyric acid is a major metabolite in colonic lumen that possesses anti-inflammatory and anti-tumour properties ( 43 ) . Milk lipid is the main dietary source of CLA.…”

Simultaneous feeding of calcium butyrate and tannin extract decreased the incidence of diarrhea and proinflammatory markers in weaned piglets

“…Butyrate acts as an acid-forming agent that has anti-inflammatory and anticarcinogenic properties [2,17]. This additive acts as the main source of energy for colonocytes and A c c e p t e d A r t i c l e enterocytes.…”

“…Thus, alternative additives to performance-enhancing antibiotics, such as organic acids and "plant extracts" should be investigated. Butyrate is a salt of a short-chain fatty acid produced in the colon by the gastrointestinal microbiota [2]. This fatty acid has antimicrobial properties, stimulates cell epithelial proliferation, acts as an anti-inflammatory agent and assists in intestinal motility [2,3].…”