Calcium and jasmonate signals mediate biosynthesis of the floral fragrance regulated by light quality in snapdragon

Yang, Yunyao; Wang, Shaojie; Leng, Pei; Wu, Jing; Hu, Zenghui

doi:10.1007/s10725-022-00807-y

Cited by 10 publications

(9 citation statements)

References 56 publications

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“…In our previous study, blue light exposure increased expression levels of AmOCS and AmMYS more than red and green light exposure, concurrently with the emission of ocimene and myrcene (Yang et al, 2022). We isolated terpenoid biosynthesisrelated genes: AmDXS, AmDXR, AmOCS, and AmMYS, and exposed snapdragon flowers with monochromatic blue light and polychromatic light without blue light in order to determine their response to blue light.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, further research is required to characterize the light responses of these plants. In our previous study using snapdragon, we found that blue light enhanced the biosynthesis of monoterpenes and the expression levels of related biosynthetic genes compared to the levels under conditions of red and green light (Yang et al, 2022). However, the regulatory mechanisms remain poorly understood.…”

Section: Introductionmentioning

confidence: 95%

“…To investigate the regulatory basis for blue light induction of the expression of terpenoid biosynthesis related genes, we used the results of transcriptome sequencing (Yang et al, 2022) to screen TFs against the reference genome (see text footnote 1) (Li et al, 2019). Two MYB TFs were found from 18 TF genes related to MYB from the RNA-seq data for different light conditions (Figure 2A).…”

Section: Expression Of Ammyb24 and Ammyb63 In Response To Blue Lightmentioning

confidence: 99%

“…Snapdragon is a model plant to explore the regulatory mechanism of floral volatile biosynthesis, and ocimene and myrcene are the main terpenoid components. Inspired by our previous finding that blue light exposure enhances the emission of ocimene and myrcene (Yang et al, 2022), we screened differentially expressed genes mediating terpenoid biosynthesis and correlated MYB TFs by RNA-seq under different light qualities to investigate the potential regulation by MYB of terpenoid biosynthesis. The interaction between CRY1 and MYB was determined as a key part of the signal transduction pathway in the terpenoid biosynthesis that is induced by blue light in snapdragon.…”

Section: Introductionmentioning

confidence: 99%

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AmMYB24 Regulates Floral Terpenoid Biosynthesis Induced by Blue Light in Snapdragon Flowers

Han

Wang

et al. 2022

Front. Plant Sci.

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Floral terpenoid volatiles are impacted by light quality. In snapdragon, blue light can significantly enhance the emissions of ocimene and myrcene and the expression of ocimene synthase (AmOCS) and myrcene synthase (AmMYS). However, the mechanisms underlying the response to blue light are largely unknown. In this study, two transcription factors (TFs), AmMYB24 and AmMYB63 were screened which showed high expression level under blue light. AmMYB24 exhibited synchronous expression with AmOCS. Moreover, AmOCS transcript expression was up-regulated in response to AmMYB24 overexpression. This activation is direct and occurs through binding of AmMYB24 to MYBCORECYCATB1 sites in the AmOCS promoter. In addition, AmMYB24 interacts with the blue light signal key receptor AmCRY1 and the transcriptional activation activity of AmMYB24 was decreased in AmCRY1 silencing flowers. Taken together, our results revealed the regulatory pathway of biosynthesis of ocimene induced by blue light mediated by AmMYB24 and AmCRY1. When snapdragon flowers were exposed to blue light, AmCRY1 was first activated, the light signal is transduced to AmMYB24 through interaction with AmCRY1, and finally AmMYB24 activates AmOCS by binding to its MYBCOREATCYCB1 motif, resulting in abundant ocimene emission.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Expression Of Ammyb24 and Ammyb63 In Response To Blue Lightmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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AmMYB24 Regulates Floral Terpenoid Biosynthesis Induced by Blue Light in Snapdragon Flowers

Han

Wang

et al. 2022

Front. Plant Sci.

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“…The sesquiterpenoids of Arabidopsis and the monoterpenes of Phalaenopsis cultivars are regulated by lights of different wavelengths [16]. Blue light signi cantly increases the expression of ocimene synthase (AmOCS) and myrcene synthase (AmMYS) compared with red and green light, which leads to increases in ocimene and myrcene emissions [17]. However, the regulatory mechanism underlying this pattern remains unclear.…”

Section: Introductionmentioning

confidence: 99%

AmPIF4 negatively regulates the blue light-induced synthesis of volatile terpenoids in snapdragon

Han

Wang

et al. 2022

Preprint

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Background Floral secondary metabolites, especially terpenoids, play a key role in plant defense and reproduction. Terpenoids are the most abundant floral volatiles, and their synthesis and release are affected by light quality. Blue light can significantly enhance the emissions of terpenoids as well as the expression of biosynthesis-related genes in snapdragon. However, the regulatory mechanism of blue light-induced terpenoid biosynthesis remains unclear. Results In this study, the transcription factor (TF) PHYTOCHROME-INTERACTING FACTOR 4 (AmPIF4) was identified and found to be lowly expressed under blue light, which was in contrast to the expression of ocimene synthase (AmOCS) and myrcene synthase (AmMYS), 1-deoxy-D-xylulose 5-phosphate (DXP) synthase (AmDXS), and DXP reductoisomerase (AmDXR). AmOCS and AmMYS expression and the relative emissions of ocimene and myrcene were up-regulated in response to AmPIF4 silencing. Moreover, AmPIF4 negatively regulated terpenoid biosynthesis by binding to the promoters of biosynthesis-related genes. The transcriptional activation activity of AmPIF4 was increased in AmCRY1-silenced flowers. In addition, AmPIF4 interacted with AmCRYs (Cryptochromes) and AmCOP1 (CONSTITUTIVELY PHOTOMORPHOGENIC 1), which are key blue light signal receptors. Conclusion Overall, our results reveal a novel molecular mechanism involving AmPIF4 that contributes to the blue light-mediated regulation of terpenoid biosynthesis in snapdragon.

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LiMYB108 is involved in floral monoterpene biosynthesis induced by light intensity in Lilium ‘Siberia’

et al. 2023

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Calcium and jasmonate signals mediate biosynthesis of the floral fragrance regulated by light quality in snapdragon

Cited by 10 publications

References 56 publications

AmMYB24 Regulates Floral Terpenoid Biosynthesis Induced by Blue Light in Snapdragon Flowers

AmMYB24 Regulates Floral Terpenoid Biosynthesis Induced by Blue Light in Snapdragon Flowers

AmPIF4 negatively regulates the blue light-induced synthesis of volatile terpenoids in snapdragon

LiMYB108 is involved in floral monoterpene biosynthesis induced by light intensity in Lilium ‘Siberia’

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