Calcium Action in Synaptic Transmitter Release

Augustine, G J; Charlton, Milton P.; Smith, Stuart

doi:10.1146/annurev.ne.10.030187.003221

Cited by 742 publications

(260 citation statements)

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“…In most cells the free Ca 2ϩ concentration rests at approxi- mately 100 nM, and following activation of cellular signaling pathways Ca 2ϩ levels are elevated about 1000-fold (30,31). We have found that intra-Golgi transport is optimal in the presence of about 100 nM free Ca 2ϩ , but that higher Ca 2ϩ concentrations strongly inhibit transport.…”

Section: Figmentioning

confidence: 74%

Regulation of Intra-Golgi Membrane Transport by Calcium

Porat

Elazar

2000

Journal of Biological Chemistry

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Calcium cations play a critical role in regulating vesicular transport between different intracellular membrane-bound compartments. The role of calcium in transport between the Golgi cisternae, however, remains unclear. Using a well characterized cell-free intra-Golgi transport assay, we now show that changes in free Ca 2؉ concentration in the physiological range regulate this transport process. The calcium-chelating agent 1,2-bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid blocked transport with an IC 50 of approximately 0.8 mM. The effect of 1,2-bis(2-aminophenoxy)-ethane-N,N,N,N-tetraacetic acid was reversible by addition of fresh cytosol and was irreversible when performed in the presence of a Ca 2؉ ionophore that depletes calcium from lumenal stores. We demonstrate here that intra-Golgi transport is stimulated by low Ca 2؉ concentrations (20 -100 nM) but is inhibited by higher concentrations (above 100 nM). Further, we show that calmodulin antagonists specifically block intraGolgi transport, implying a role for calmodulin in mediating the effect of calcium. Our results suggest that Ca 2؉

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Section: Figmentioning

confidence: 74%

Regulation of Intra-Golgi Membrane Transport by Calcium

Porat

Elazar

2000

Journal of Biological Chemistry

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“…ions (Katz, 1969;Augustine et al, 1987). Presumably, Ca 2ϩ ions initiate conformational changes in proteins which ultimately catalyze membrane fusion.…”

Section: Exocytosis Of Synaptic Vesicles Is Strictly Controlled By Ca 2ϩmentioning

confidence: 99%

Ca2+ Regulates the Interaction between Synaptotagmin and Syntaxin 1

Chapman

Hanson

et al. 1995

Journal of Biological Chemistry

355

286

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“…Neurotransmitter release is ultimately triggered by cytoplasmic calcium concentrations of the order of 100 ~tM, which are attained transitorily in close proximity to voltage gated calcium channels ( [3], reviewed in [4]). Co-immunoprecipitation experiments and calcium channel purification have indicated that synaptotagmin and syntaxin can associate with N-type calcium channels [5,6].…”

Section: Introductionmentioning

confidence: 99%