Calcitriol affects hCG gene transcription in cultured human syncytiotrophoblasts

Barrera, David; Avila, Euclides; Hernández, Guillermo; Méndez, Isabel García; González, López; Halhali, Ali; Larrea, Fernando; Escobedo-Morales, A.; Díaz, Lorenza

doi:10.1186/1477-7827-6-3

Cited by 138 publications

(79 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…37 Accordingly, although our data do not allow for conclusions on cause --effect relationships, it seems biological plausible that low P-25OHD concentrations may exert adverse effects on the course of a pregnancy. 37,38 Controversies exist on whether P-25OHD is affected by pregnancy per se. Holmes et al 26 found lower P-25OHD concentrations in women during their second and third trimester compared with non-pregnant controls, whereas Hillman et al 27 found no such pregnancy-induced decrease in P-25OHD.…”

Section: Discussionmentioning

confidence: 83%

“…This is further supported by in vitro data showing that the vitamin D-activating enzyme (CYP27B1) and the vitamin D receptor are expressed in the placental syncytiotrophoblastic layer. 37 Moreover, calcitriol might affect the expression and secretion of human chorionic gonadotropin, progesterone and estradiol in cultured trophoblasts. 37 Accordingly, although our data do not allow for conclusions on cause --effect relationships, it seems biological plausible that low P-25OHD concentrations may exert adverse effects on the course of a pregnancy.…”

Section: Discussionmentioning

confidence: 99%

“…37 Moreover, calcitriol might affect the expression and secretion of human chorionic gonadotropin, progesterone and estradiol in cultured trophoblasts. 37 Accordingly, although our data do not allow for conclusions on cause --effect relationships, it seems biological plausible that low P-25OHD concentrations may exert adverse effects on the course of a pregnancy. 37,38 Controversies exist on whether P-25OHD is affected by pregnancy per se.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of 25OHD concentrations on chances of pregnancy and pregnancy outcomes: a cohort study in healthy Danish women

et al. 2012

View full text Add to dashboard Cite

BACKGROUND/OBJECTIVES: Plasma 25-hydroxyvitamin D (P-25OHD) concentrations may affect pregnancy outcomes. To elucidate this further, we studied the effects of pre-conception P-25OHD concentrations on chances for pregnancy as well as the effects of P-25OHD during pregnancy on the risk of miscarriage, birth weight and length, Apgar score and head circumference. Moreover, we studied whether pregnancy and breastfeeding patterns affect maternal P-25OHD concentrations. SUBJECTS/METHODS: A total of 153 healthy Caucasian women with pregnancy plans were followed with measurements performed before pregnancy, at pregnancy weeks 11±2, 22±1 and 35±2 as well as 15±7, 129±12 and 280±15 days postpartum. Furthermore, 75 non-pregnant, age-matched women were followed in parallel as controls. RESULTS: The 203 women were aged 29 (25 --35) years. At baseline, median P-25OHD was 59 nmol/l. Of these women, 31% had P-25OHD o50 nmol/l, whereas 12% had levels above 80 nmol/l. Within B6 months after inclusion, 63% conceived. P-25OHD was not associated with chances of conceiving or overall risk of miscarriage. However, women with a miscarriage in their second trimester (n ¼ 3) had lower P-25OHD concentrations at measurements performed in the first trimester compared with women without a miscarriage (P ¼ 0.03). P-25OHD before or during pregnancy was not associated with gestational length or infant parameters. Adjustments for possible confounders did not change the result. During pregnancy, P-25OHD changed significant over time, but similar changes occurred within the control group, indicating no effect of pregnancy per se (P ¼ 0.59). Overall, P-25OHD did not differ according to length of breastfeeding at 2 weeks, and 4 and 9 months postpartum, although women breastfeeding for 49 months had lower P-25OHD levels at the last visit compared with the controls. CONCLUSION: P-25OHD concentrations did not affect fertility or pregnancy outcomes, although low P-25OHD may be associated with an increased risk of late miscarriage. 1 In female rats, low P-25OHD concentration has been associated with gonadal insufficiency, 2 although it is unclear whether this is due to the effects of hypocalcemia rather than a direct effect of P-25OHD. The vitamin D receptor is expressed in human sperm cells, and 1,25-dihydroxyvitamin D concentration may affect sperm survival and the acquisition of fertilizing ability of sperm cells.

show abstract

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of 25OHD concentrations on chances of pregnancy and pregnancy outcomes: a cohort study in healthy Danish women

et al. 2012

View full text Add to dashboard Cite

show abstract

“…We conducted our experiments with 0.1 or 10 nM vitamin D 3 as described in several publications (5,18), approximating physiologic vitamin D 3 serum concentrations in pregnant and nonpregnant women (0.1 nM; Refs. 18,24).…”

Section: Discussionmentioning

confidence: 99%

Vitamin D improves the angiogenic properties of endothelial progenitor cells

Grundmann

Haidar

Placzko

et al. 2012

American Journal of Physiology-Cell Physiology

131

112

View full text Add to dashboard Cite

The main pathogenic feature of preeclampsia is maternal endothelial dysfunction that results from impaired angiogenesis and reduced endothelial repair capacity. In addition, preeclampsia risk is associated with vitamin D deficiency. We hypothesized that vitamin D3 stimulates proangiogenic properties of endothelial colony-forming cells (ECFCs). ECFCs were obtained and cultured from cord blood and characterized by immunocytochemistry and flow cytometry. Proliferation, total length of tubule formation on Matrigel, expression of VEGF mRNA, and pro-matrix metalloproteinases (MMP)-2 activity were assessed after treatment of ECFCs with vitamin D 3. Specificity of the observed effects was tested by blocking the vitamin D receptor (VDR) or the VEGF signaling pathway. ECFCs treated with 10 nM vitamin D3 showed a 1.27 times higher tubule formation compared with vehicle-treated controls (1.27 Ϯ 0.19) as well as a 1.36 times higher proliferation rate (1.36 Ϯ 0.06). Vitamin D3 induced pro-MMP-2 activity (1.29 Ϯ 0.17) and VEGF mRNA levels (1.74 Ϯ 0.73) in ECFCs. VDR blocking by pyridoxal-5-phosphate (0.73 Ϯ 0.19) or small interfering RNA (0.75 Ϯ 0.17) and VEGF inhibition by Su5416 (0.56 Ϯ 0.16) or soluble fms-like tyrosine kinase-1 (0.7 Ϯ 0.14) reduced tubule formation and pro-MMP-2 activity (pyridoxal-5-phosphate: 0.84 Ϯ 0.09; Su5416: 0.79 Ϯ 0.11; or sFlt: 0.88 Ϯ 0.13). This effect was neutralized by vitamin D3. Consequently, vitamin D3 significantly promoted angiogenesis in ECFCs in vitro possibly due to an increase in VEGF expression and pro-MMP-2 activity. Since angiogenesis is a crucial feature in the pathophysiology of preeclampsia these findings could explain the positive influence of vitamin D3 in reducing preeclampsia risk. vitamin D; preeclampsia; ECFC; angiogenesis; VEGF VITAMIN D, PRIMARILY KNOWN for its important role in calcium homeostasis and bone metabolism, influences the cardiovascular system through unclear mechanisms (33). Vitamin D deficiency is associated with increased all-cause and cardiovascular disease mortality, coronary heart disease, and various cardiovascular risk factors (33).Preeclampsia, a pregnancy-specific disorder that affects 3-7% of all pregnancies, is a major cause of maternal and fetal morbidity and mortality (43) and is associated with an increased risk for cardiovascular events later in life (6). Endothelial dysfunction underlies the hypertension, proteinuria, and multiorgan damage that occur during preeclampsia. The mechanisms that contribute to the disturbed endothelial homeostasis in the pathophysiology of preeclampsia remain unclear (41,46). Compared with normal pregnancies, preeclampsia is characterized by marked changes in vitamin D and calcium metabolism (3). Epidemiological studies have demonstrated an association between low maternal vitamin D levels and the incidence of preeclampsia (23, 27) and suggest vitamin D deficiency to be an independent risk factor for the development of preeclampsia (7). Moreover, vitamin D supplementation studies also showed protective effects on pr...

show abstract

“…Было показано, что 1,25(OH) 2 D 3 регулирует выделение и секре-цию хорионического гонадотропина человека в синтициотрофобласте [18] и увеличивает плацентарное производство половых стероид-ных гормонов [19]. Оказалось, что кальци триол способствует транспорту кальция в плацен-ту [20], стимулирует выделение плацентарного лактогена [21], а также регулирует экспрессию HOXA10 (гена, определяющего развитие поло-вых органов) в стромальных клетках эндомет-рия человека [22].…”

Section: роль витамина D в регуляции репродуктивной функцииunclassified

The role of vitamin D in the pathogenesis of genital endometriosis

Denisova

Yarmolinskaya

2017

Z.akus.zen.bolezn

View full text Add to dashboard Cite

The influence of vitamin D on the women’s health is undeniable. Here we review the modern classification of vitamin D forms and receptors, the current understanding of genomic and non-genomic regulation pathways of vitamin D synthesis. It was found that a vitamin D deficiency related to the development of many diseases, negative impact on reproductive function and pregnancy outcomes. Nowadays, vitamin D is concerned as a prohormone that has multiple effects. Special attention is given to the role of vitamin D in the pathogenesis of genital endometriosis. It is known that vitamin D has anti-inflammatory, antiproliferative and immunomodulating effects, which allow it to be considered as a forward-looking target therapy in endometriosis.

show abstract

Calcitriol affects hCG gene transcription in cultured human syncytiotrophoblasts

Cited by 138 publications

References 34 publications

Effects of 25OHD concentrations on chances of pregnancy and pregnancy outcomes: a cohort study in healthy Danish women

Effects of 25OHD concentrations on chances of pregnancy and pregnancy outcomes: a cohort study in healthy Danish women

Vitamin D improves the angiogenic properties of endothelial progenitor cells

The role of vitamin D in the pathogenesis of genital endometriosis

Contact Info

Product

Resources

About