Calcitriol [1, 25[OH]2 D3] pre- and post-treatment suppresses inflammatory response to influenza A (H1N1) infection in human lung A549 epithelial cells

Khare, Drirh; Godbole, Nachiket M.; Pawar, Shailesh D.; Mohan, Vishwa; Pandey, Gaurav; Gupta, Sushil K.; Kumar, Deepak; Dhole, Tapan N.; Godbole, Madan M.

doi:10.1007/s00394-012-0449-7

Cited by 126 publications

(104 citation statements)

References 37 publications

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“…19,35,36 The contribution of autophagy to MHC-II-restricted presentation by DCs after H1N1 virus infection has also been evaluated. However, before this report, autophagy in DCs after H1N1 virus infection had not been examined, and whether autophagy could be induced in DCs upon H1N1 infection was unclear.…”

Section: Discussionmentioning

confidence: 99%

Autophagy is involved in regulating the immune response of dendritic cells to influenza A (H1N1) pdm09 infection

Zang¹,

Chen

Lin

et al. 2016

Immunology

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SummaryAutophagy can mediate antiviral immunity. However, it remains unknown whether autophagy regulates the immune response of dendritic cells (DCs) to influenza A (H1N1) pdm09 infection. In this study, we found that infection with the H1N1 virus induced DC autophagy in an endocytosis-dependent manner. Compared with autophagy-deficient Beclin-1 +/À mice, we found that bone-marrow-derived DCs from wild-type mice (WT BMDCs) presented a more mature phenotype on H1N1 infection. Wildtype BMDCs secreted higher levels of interleukin-6 (IL-6), tumour necrosis factor-a ( Our data indicate that autophagy is important in the regulation of the DC immune response to H1N1 infection, thereby extending our understanding of host immune responses to the virus.

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Section: Discussionmentioning

confidence: 99%

Autophagy is involved in regulating the immune response of dendritic cells to influenza A (H1N1) pdm09 infection

Zang¹,

Chen

Lin

et al. 2016

Immunology

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“…This is in agreement with the conclusion given by Grant and Giovanucci [48], who discussed, regarding the data showing that suppressing proinflammatory cytokines by vitamin D did not reduce the risk of death in mice infected with H5N1 viruses, that such an effect should not be applied to the H1N1 infections in humans because of the differences in immune response. The anti-inflammatory activity of 1α,25(OH) 2 D on A549 infected with H1N1 was also shown by Khare et al [50]. The authors noted that 1α,25(OH) 2 D treatment prior to or post-infection downregulated IL-6 and IL-8 RNA levels and decreased the levels of infection-induced TNFα, IFNβ, and ISG15.…”

Section: Vitamin D Versus Influenza—prevention or Therapy?mentioning

confidence: 56%

“…The release of proinflammatory cytokines by the influenza virus appeared to correlate with the severity of illness [24]. Khare et al [50] noted that treatment of human lung A549 epithelial cells with 100 or 30 nM of 1α,25(OH) 2 D prior to or post-H1N1 (influenza A virus) exposure significantly decreased the levels of infection-induced TNFα, IFNβ, and IFN-stimulated gene-15 (ISG15) and downregulated IL-8 and IL-6 RNA levels. Following Helming et al [51], 1α,25(OH) 2 D also potentially diminishes the proinflammatory cytokine production by the modulation of macrophages, which prevents them from the secretion of too many cyto- and chemokines.…”

Section: Vitamin D Versus Influenza—prevention or Therapy?mentioning

confidence: 99%

Vitamin D and Influenza—Prevention or Therapy?

Gruber-Bzura

2018

IJMS

180

168

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Vitamin D generates many extraskeletal effects due to the vitamin D receptor (VDR) which is present in most tissues throughout the body. The possible role of vitamin D in infections is implied from its impact on the innate and adaptive immune responses. A significant effect is also the suppression of inflammatory processes. Because vitamin D could be acknowledged as a “seasonal stimulus”, as defined by R. Edgar Hope-Simpson, it would be crucial to prove it from a potential easy and cheap prophylaxis or therapy support perspective as far as influenza infections are concerned. The survey of the literature data generates some controversies and doubts about the possible role of vitamin D in the prevention of influenza virus. The most important point is to realise that the broad spectrum of this vitamin’s activity does not exclude such a possibility. According to most of the authors, more randomized controlled trials with effective, large populations are needed to explore the preventive effect of vitamin D supplementation on viral influenza infections.

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“…tion[24], we then inspected the mRNA levels of M protein after treat-353 ment with peptides. The results displayed a dramatically reduced 354 level of mRNA expression on treatment with both C12-KKWK and 355…”

mentioning

confidence: 99%

Super short membrane-active lipopeptides inhibiting the entry of influenza A virus

Wang

Dong

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Influenza A viruses (IAV) are significant pathogens that result in millions of human infections and impose a substantial health and economic burdens worldwide. Due to the limited anti-influenza A therapeutics available and the emergence of drug resistant viral strains, it is imperative to develop potent anti-IAV agents with different mode of action. In this study, by applying a pseudovirus based screening approach, two super short membrane-active lipopeptides of C12-KKWK and C12-OOWO were identified as effective anti-IAV agents with IC50 value of 7.30±1.57 and 8.48±0.74 mg/L against A/Puerto Rico/8/34 strain, and 6.14±1.45 and 7.22±0.67 mg/L against A/Aichi/2/68 strain, respectively. The mechanism study indicated that the anti-IAV activity of these peptides would result from the inhibition of virus entry by interacting with HA2 subunit of hemagglutinin (HA). Thus, these peptides may have potentials as lead peptides for the development of new anti-IAV therapeutics to block the entry of virus into host cells.

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Calcitriol [1, 25[OH]2 D3] pre- and post-treatment suppresses inflammatory response to influenza A (H1N1) infection in human lung A549 epithelial cells

Abstract: The findings provide support for the initiation of vitamin D supplementation program to VDD populations in reducing the severity of influenza.

Cited by 126 publications

References 37 publications

Autophagy is involved in regulating the immune response of dendritic cells to influenza A (H1N1) pdm09 infection

Autophagy is involved in regulating the immune response of dendritic cells to influenza A (H1N1) pdm09 infection

Vitamin D and Influenza—Prevention or Therapy?

Super short membrane-active lipopeptides inhibiting the entry of influenza A virus

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