Calcitonin gene-related peptide regulates amino acid absorption across rat jejunum

Barada, Kassem; Saadé, Nayef E.; Atweh, Samir; Khoury, Carmen I.; Nassar, Camille F.

doi:10.1016/s0167-0115(00)00103-8

Cited by 14 publications

(14 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First of all, this peptide takes part in the conduction of sensory and pain impulses [19,20], but it also increases a blood flow in mesenteric vessels [21], inhibits gastric acid secretion, and regulates the absorption of nutrients from the gut [22]. Other important functions of CGRP are the ability to stimulate the secretion of other neurotransmitters, including somatostatin and nitric oxide [38], and the protection of endothelial cells against damage [24].…”

Section: Discussionmentioning

confidence: 99%

“…Within the intestine CGRP is regarded as the key neurotransmitter and/or neuromodulator of the ENS participating in sensory and nociceptive transmission and a marker of the intrinsic primary afferent neurons [19,20]. It is also known that this substance inhibits gastric acid secretion, increases mesenteric blood flow, protects intestinal mucosa and, possibly, exerts relaxatory effects on the gastrointestinal muscle layer [21,22,23]. Moreover, some studies show that CGRP is involved in the regeneration of nervous tissue after injury [24].…”

Section: Introductionmentioning

confidence: 99%

“…Due to the significant sensitivity of the ENS to the presence of harmful agents in the GI tract [28] and the variety of functions that CGRP exerts on the intestinal regulatory processes [20,21,22], these changes may represent the first subclinical symptoms of the damage caused by the mycotoxins studied. The obtained results will enable the development of knowledge about the actions of low doses of ZEN and T-2 toxin on living organisms this study is of particular interest, given that the pig is considered to be an excellent animal model of human physiology [31].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon

Makowska

Obremski

Zielonka

et al. 2017

Toxins

View full text Add to dashboard Cite

The enteric nervous system (ENS) can undergo adaptive and reparative changes in response to physiological and pathological stimuli. These manifest primarily as alterations in the levels of active substances expressed by the enteric neuron. While it is known that mycotoxins can affect the function of the central and peripheral nervous systems, knowledge about their influence on the ENS is limited. Therefore, the aim of the present study was to investigate the influence of low doses of zearalenone (ZEN) and T-2 toxin on calcitonin gene related peptide-like immunoreactive (CGRP-LI) neurons in the ENS of the porcine descending colon using a double immunofluorescence technique. Both mycotoxins led to an increase in the percentage of CGRP-LI neurons in all types of enteric plexuses and changed the degree of co-localization of CGRP with other neuronal active substances, such as substance P, galanin, nitric oxide synthase, and cocaine- and amphetamine-regulated transcript peptide. The obtained results demonstrate that even low doses of ZEN and T-2 can affect living organisms and cause changes in the neurochemical profile of enteric neurons.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon

Makowska

Obremski

Zielonka

et al. 2017

Toxins

View full text Add to dashboard Cite

show abstract

“…CGRP is widely distributed in the enteric neurons and in primary sensory afferents in the gut (23,38). Previous results from our group provided direct evidence on the role of CGRP in decreasing jejunal amino acid absorption through direct (intrinsic) and indirect (central) neural mechanisms (7). Thus one might speculate that CGRP release triggered by ileal Ala may inhibit jejunal amino acid absorption through two possible mechanisms: either locally, by the release of CGRP from CSPA peripheral terminals, or centrally, by alteration of the function of vagal preganglionic neurons through CGRP released by the central terminals of CSPA fibers.…”

Section: Discussionmentioning

confidence: 84%

Amino acids in the rat intestinal lumen regulate their own absorption from a distant intestinal site

Mourad

Barada

Khoury

et al. 2009

American Journal of Physiology-Gastrointestinal and Liver Physi

View full text Add to dashboard Cite

Intestinal nutrient transport is altered in response to changes in dietary conditions and luminal substrate level. It is not clear, however, whether an amino acid in the intestinal lumen can acutely affect its own absorption from a distant site. Our aim is to study the effect of an amino acid present in rat small intestinal segment on its own absorption from a proximal or distal site and elucidate the underlying mechanisms. The effect of instillation of alanine (Ala) in either jejunum or ileum on its own absorption at ileal or jejunal level was examined in vivo. The modulation of this intestinal regulatory loop by the following interventions was studied: tetrodotoxin (TTX) added to Ala, subdiaphragmatic vagotomy, chemical ablation of capsaicin-sensitive primary afferent (CSPA) fibers, and IV administration of calcitonin gene-related peptide (CGRP) antagonist. In addition, the kinetics of jejunal Ala absorption and the importance of Na+-dependent transport were studied in vitro after instilling Ala in the ileum. Basal jejunal Ala absorption [0.198 +/- 0.018 micromol x cm(-1) x 20 min(-1) (means +/- SD)] was significantly decreased with the instillation of 20 mM Ala in the ileum or in an adjacent distal jejunal segment (0.12 +/- 0.015; P < 0.0001 and 0.138 +/- 0.014; P < 0.002, respectively). Comparable inhibition was observed in the presence of proline in the ileum. Moreover, basal Ala absorption from the ileum (0.169 +/- 0.025) was significantly decreased by the presence of 20 mM Ala in the jejunum (0.103 +/- 0.027; P < 0.01). The inhibitory effect on jejunal Ala absorption was abolished by TTX, subdiaphragmatic vagotomy, neonatal capsaicin treatment, and CGRP antagonism. In vitro studies showed that Ala in the ileum affects Na+-mediated transport and increases K(m) without affecting Vmax. Intraluminal amino acids control their own absorption from a distant part of the intestine, by affecting the affinity of the Na+-mediated Ala transporter, through a neuronal mechanism that involves CSPA and CGRP.

show abstract

“…15) In addition, it was demonstrated that capsaicin decreased intestinal alanine absorption by stimulating afferent neurons to release CGRP and VIP. [16][17][18] Recently, it was suggested that TRPV1 is expressed not only in nerve cells, but also in gastric mucosal tissues and intestinal epithelia. [19][20][21][22] As capsaicin associates with TRPV1, thus increasing Ca 2ϩ influx, it may alter the intracellular Ca 2ϩ concentration in these cells.…”

mentioning

confidence: 99%

Effects of Capsaicin on Intestinal Cephalexin Absorption in Rats

Komori

Aiba

Sugiyama

et al. 2007

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Calcitonin gene-related peptide regulates amino acid absorption across rat jejunum

Cited by 14 publications

References 25 publications

The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon

The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon

Amino acids in the rat intestinal lumen regulate their own absorption from a distant intestinal site

Effects of Capsaicin on Intestinal Cephalexin Absorption in Rats

Contact Info

Product

Resources

About