Calcitonin gene-related peptide dynamics in rat dorsal root ganglia and spinal cord following different sciatic nerve injuries

Zheng, Lin; Wang, Rui; Xu, Yuanzhong; Yi, Xi Nan; Zhang, Jian Wei; Zeng, Zhi Cheng

doi:10.1016/j.brainres.2007.10.044

Cited by 70 publications

(76 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sensitivity to pain and injury severity is also evident in spinal CGRP expression (Fig. 5), which also increases after a host of painful peripheral traumas and inflammatory stimuli [18,50,56,69,92]. In contrast, it decreases after a painful root compression [37,92], most likely from axonal degeneration in the root decreasing trafficking to the dorsal horn.…”

Section: Discussionmentioning

confidence: 97%

“…5), which also increases after a host of painful peripheral traumas and inflammatory stimuli [18,50,56,69,92]. In contrast, it decreases after a painful root compression [37,92], most likely from axonal degeneration in the root decreasing trafficking to the dorsal horn. Because CGRP enhances PKC-mediated signaling to increase neuronal hyperexcitability [44,58,74] and because only peripheral PKCe is upregulated after nonpainful 15 Hz WBV, it is likely that several responses sustain pain from WBV.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Whole-body Vibration at Thoracic Resonance Induces Sustained Pain and Widespread Cervical Neuroinflammation in the Rat

Zeeman

Kartha

Jaumard

et al. 2015

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

Background Whole-body vibration (WBV) is associated with back and neck pain in military personnel and civilians. However, the role of vibration frequency and the physiological mechanisms involved in pain symptoms are unknown. Questions/purposes This study asked the following questions: (1) What is the resonance frequency of the rat spine for WBV along the spinal axis, and how does frequency of WBV alter the extent of spinal compression/ extension? (2) Does a single WBV exposure at resonance induce pain that is sustained? (3) Does WBV at resonance alter the protein kinase C epsilon (PKCe) response in the dorsal root ganglia (DRG)? (4) Does WBV at resonance alter expression of calcitonin gene-related peptide (CGRP) in the spinal dorsal horn? (5) Does WBV at resonance alter the spinal neuroimmune responses that regulate pain? Methods Resonance of the rat (410 ± 34 g, n = 9) was measured by imposing WBV at frequencies from 3 to 15 Hz. Separate groups (317 ± 20 g, n = 10/treatment) underwent WBV at resonance (8 Hz) or at a nonresonant frequency (15 Hz). Behavioral sensitivity was assessed throughout to measure pain, and PKCe in the DRG was quantified as well as spinal CGRP, glial activation, and cytokine levels at Day 14. Results Accelerometer-based thoracic transmissibility peaks at 8 Hz (1.86 ± 0.19) and 9 Hz (1.95 ± 0.19, mean difference [MD] 0.290 ± 0.266, p \ 0.03), whereas the video-based thoracic transmissibility peaks at 8 Hz (1.90 ± 0.27), 9 Hz (2.07 ± 0.20), and 10 Hz (1.80 ± 0.25, MD 0.359 ± 0.284, p \ 0.01). WBV at 8 Hz produces more cervical extension (0.745 ± 0.582 mm, MD 0.242 ± 0.214, p \ 0.03) and compression (0.870 ± 0.676 mm, MD 0.326 ± 0.261, p \ 0.02) than 15 Hz (extension, 0.503 ± 0.279 mm; compression, 0.544 ± 0.400 mm). Pain is longer lasting (through Day 14) and more robust (p \ 0.01) after WBV at the resonant frequency (8 Hz) compared with 15 Hz WBV. PKCe in the nociceptors of the DRG increases according to the severity of WBV with greatest increases after 8 Hz WBV (p \ 0.03). However, spinal CGRP, cytokines, and glial activation are only evident after painful WBV at resonance. Conclusions WBV at resonance produces long-lasting pain and widespread activation of a host of nociceptive and neuroimmune responses as compared with WBV at a nonresonance condition. Based on this work, future investigations into the temporal and regional neuroimmune response to resonant WBV in both genders would be useful.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Whole-body Vibration at Thoracic Resonance Induces Sustained Pain and Widespread Cervical Neuroinflammation in the Rat

Zeeman

Kartha

Jaumard

et al. 2015

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

show abstract

“…CGRP-immunoreactivity (IR) in dorsal horn in the lumbar spinal cord was detected by immunohistochemistry as described previously [29]. The immunoproducts were confirmed to be positive-labelled if their density level was over five times the background levels.…”

Section: Image Analysismentioning

confidence: 99%

Novel use of biodegradable casein conduits for guided peripheral nerve regeneration

Hsiang

Tsai

et al. 2011

J. R. Soc. Interface.

View full text Add to dashboard Cite

Recent advances in nerve repair technology have focused on finding more biocompatible, nontoxic materials to imitate natural peripheral nerve components. In this study, casein protein cross-linked with naturally occurring genipin (genipin-cross-linked casein (GCC)) was used for the first time to make a biodegradable conduit for peripheral nerve repair. The GCC conduit was dark blue in appearance with a concentric and round lumen. Water uptake, contact angle and mechanical tests indicated that the conduit had a high stability in water and did not collapse and cramped with a sufficiently high level of mechanical properties. Cytotoxic testing and terminal deoxynucleotidyl transferase dUTP nick-end labelling assay showed that the GCC was non-toxic and non-apoptotic, which could maintain the survival and outgrowth of Schwann cells. Non-invasive real-time nuclear factor-kB bioluminescence imaging accompanied by histochemical assessment showed that the GCC was highly biocompatible after subcutaneous implantation in transgenic mice. Effectiveness of the GCC conduit as a guidance channel was examined as it was used to repair a 10 mm gap in the rat sciatic nerve. Electrophysiology, labelling of calcitonin gene-related peptide in the lumbar spinal cord, and histology analysis all showed a rapid morphological and functional recovery for the disrupted nerves. Therefore, we conclude that the GCC can offer great nerve regeneration characteristics and can be a promising material for the successful repair of peripheral nerve defects.

show abstract

“…trkA is a high affinity receptor for nerve growth factor (NGF) [21] , and NGF has been shown to promote the survival of different types of neurons in coordination with HGF [26] . Therefore, protein levels of NGF were also detected within the sciatic nerve following SNL [27] . Our findings indicate that the HGF/c-Met system is spatiotemporally regulated following peripheral nerve injury, and the unique signaling pattern for this system further suggests a role for HGF/c-Met in promoting the survival of adult DRG sensory neurons and spinal motoneurons.…”

Section: Introductionmentioning

confidence: 97%

“…As described previously [27] , dissected samples were combined with 5 vol (w/v) of ice-cold lysis buffer (150 m M NaCl, 1% Nonidet P40, 0.5% sodium deoxycholate, 0.1% SDS, and 1 m M sodium orthovanadate in 50 m M Tris-HCl (pH 7.4)), homogenized using a Dounce homogenizer, and centrifuged (12,000 g ) for 15 min at 4 ° C. After 30 min on ice, supernatants were collected and immediately stored at -80 ° C until needed.…”

Section: Tissue Processingmentioning

confidence: 99%

Expression of HGF/c-Met Is Dynamically Regulated in the Dorsal Root Ganglions and Spinal Cord of Adult Rats following Sciatic Nerve Ligation

Zheng

Wang

et al. 2010

Neurosignals

Self Cite

View full text Add to dashboard Cite

show abstract

Calcitonin gene-related peptide dynamics in rat dorsal root ganglia and spinal cord following different sciatic nerve injuries

Cited by 70 publications

References 37 publications

Whole-body Vibration at Thoracic Resonance Induces Sustained Pain and Widespread Cervical Neuroinflammation in the Rat

Whole-body Vibration at Thoracic Resonance Induces Sustained Pain and Widespread Cervical Neuroinflammation in the Rat

Novel use of biodegradable casein conduits for guided peripheral nerve regeneration

Expression of HGF/c-Met Is Dynamically Regulated in the Dorsal Root Ganglions and Spinal Cord of Adult Rats following Sciatic Nerve Ligation

Contact Info

Product

Resources

About