Calcitonin effects on isolated bone cells

Shlossman, Marc; Brown, M.; Shapiro, Edward P.; Dziak, Rosemary

doi:10.1007/bf02411232

Cited by 44 publications

(11 citation statements)

References 22 publications

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“…Again, osteoblast-like cells were found to be located deeper in the bone. Due to the anatomic position of cells in fetal and neonatal rodent calvaria, both of these methods allow an enrichment for distinct cell types, as also confirmed by other groups [11][12][13][14]. Since it has not been possible to identify osteoblasts in culture by simple microscopic examination, Luben et al [15] have suggested characterizing cultured bone cells by their biochemical properties and hormone responsiveness.…”

mentioning

confidence: 87%

Insulin-like growth factors stimulate synthesis of nucleic acids and glycogen in cultured calvaria cells

1983

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A (sub)population of cells obtained from newborn rat calvaria by (sequential) collagenase digestion is grown to confluence in serum-containing medium. These cells are osteoblast-like with respect to high alkaline phosphatase activity and marked responsiveness (cAMP) to parathormone. Insulin-like growth factors (IGFs) enhance net incorporation of the labeled precursors thymidine, uridine, and glucose into the respective macromolecules DNA, RNA, and glycogen. Human IGF I is five times as potent as IGF II in evoking these anabolic responses in cultured rat calvaria cells. In contrast to insulin, the factors are effective in concentrations in which they are present in serum.

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mentioning

confidence: 87%

Insulin-like growth factors stimulate synthesis of nucleic acids and glycogen in cultured calvaria cells

1983

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“…However, these studies indicate their modes of action on isolated bone cells are not identical. We have previously shown that in osteoclastic cells, CT decreases calcium uptake and increases cAMP with no effect on calcium uptake or cAMP production in osteoblastic populations [10]. By contrast, BL-5583 decreased calcium uptake in all populations of cells and did not increase cAMP levels in any bone cell population.…”

Section: Discussionmentioning

confidence: 90%

“…period; III and IV obtained after the third and fourth periods, respectively. Populations I and II were previously characterized osteoclastic by high acid phosphatase levels and the ability to resorb devitalized bone in vitro [10]. Populations III and IV were characterized osteoblastic by high alkaline phosphatase levels and the lack of in vitro resorptive ability [10].…”

Section: Homogeneous Bone Cell Isolation Proceduresmentioning

confidence: 98%

“…Enriched populations of bone cells were isolated by sequential collagenase digestion of 1-2 day-old newborn rat (SpragueDawley) calvariae using an adaption of the Wong and Cohn [9] technique described previously [10]. The procedure was identical to that described above for obtaining heterogeneous preparations, except the cells were harvested from the collagenase solution every 15 rain for a total of 60 rain.…”

Section: Homogeneous Bone Cell Isolation Proceduresmentioning

confidence: 99%

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Benzo(B)Thiophene-2-Carboxylic acid: Calcium uptake and cyclic AMP production in isolated bone cells

et al. 1984

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The purpose of the present study was to investigate the mechanism of action on bone of Benzo(B)Thiophene-2-Carboxylic Acid (BL-5583). BL-5583, at a dose range of 0.01-100 micrograms/ml, inhibited spontaneous as well as A23187 and PTH-induced bone resorption in tissue culture. This compound also decreased calcium uptake in both osteoclastic and osteoblastic enriched bone cell populations obtained by sequential collagenase digestion of 1-2 day newborn rat calvariae. The decrease occurred after a 5 min. incubation with 45Ca and BL-5583. The effective dose range was 0.01-100 micrograms/ml. No effect on leucine incorporation or lactic acid production by bone cells was observed. BL-5583 also induced a transient decrease in calcium uptake in skin cells isolated from fetal rats by collagenase digestion, suggesting a lack of tissue specificity for this compound. No effect on cyclic AMP in isolated bone cells was observed with the same dose range that produced a calcium effect.

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“…The first two populations (I and II) released in a series of four 15-min collagenase digestions had properties distinct from the populations (III and IV) released during the last 2 collagenase incubation periods [16]. Populations I and II had acid phosphatase levels higher than III and IV, responded to calcitonin with an increase in cyclic AMP, and resorbed devitalized bone in vitro.…”

Section: Studies With Characterized Bone Cell Populationsmentioning

confidence: 97%

Prostaglandin E2 binding and cyclic AMP production in isolated bone cells

et al. 1983

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The binding of prostaglandin E2 (PGE2) to bone cells was studied to provide direct evidence for the existence of specific receptors in bone. Bone cells were isolated by collagenase digestion of fetal and newborn rat calvaria. Isolated cells were incubated with 3H-PGE2 and collected on Millipore filters. Specific binding was determined by subtracting the binding that occurred with 10(-6) M non-radioactive PGE2 and 3H-PGE2 from that with 3H-PGE2 alone. With heterogeneous cell preparations and at PGE2 concentrations from 10(-9) - 1.7 X 10(-8) M at 37 degrees C, specific binding reached steady state within 10 min. Bound 3H-PGE2 was displaced by the addition of increasing amounts of unlabeled PGE2. Inhibition of PGE2 binding was observed with PGE1 and the endoperoxide analog, U44069, but not with PGF2 alpha, a lipopolysaccharide, or 13,14-dihydro 15-keto PGE2. Studies with bone cell populations, obtained by sequential digestions, indicated that an osteoclastic population binds 30-fold more PGE2 than osteoblastic cells. Scatchard analyses revealed that the osteoclastic cells have an affinity constant for PGE2 binding similar to that obtained with heterogeneous populations. However, the PGE2 binding capacity in this osteoclastic population was fivefold greater than in the heterogeneous population. The osteoclastic population responded with an increase in cyclic AMP to lower concentrations of PGE2 than the osteoblastic populations. These studies suggest that differences in the binding capacity of PGE2 receptors exist among bone cell-types and that these differences are reflected in the cellular cyclic AMP response.

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Calcitonin effects on isolated bone cells

Cited by 44 publications

References 22 publications

Insulin-like growth factors stimulate synthesis of nucleic acids and glycogen in cultured calvaria cells

Insulin-like growth factors stimulate synthesis of nucleic acids and glycogen in cultured calvaria cells

Benzo(B)Thiophene-2-Carboxylic acid: Calcium uptake and cyclic AMP production in isolated bone cells

Prostaglandin E2 binding and cyclic AMP production in isolated bone cells

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